TAK1 (MAP3K7) (NM_003188) Human Untagged Clone

CAT#: SC118119

MAP3K7 (untagged)-Human mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant A

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAK1
• Synonyms: CSCF; FMD2; MEKK7; TAK1; TGF1a
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003188 edited
  ATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGATCGAA
  GCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAG
  GTTGTTGGAAGAGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTT
  GCTATTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAG
  TTATCCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAATCCAGTG
  TGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGTGCTGCATGGTGCTGAA
  CCATTGCCATATTATACTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGA
  GTGGCTTATCTTCACAGCATGCAACCCAAAGCGCTAATTCACAGGGACCTGAAACCACCA
  AACTTACTGCTGGTTGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGT
  GACATTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCTGAAGTT
  TTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTGGGGTATTATTCTTTGG
  GAAGTGATAACGCGTCGGAAACCCTTTGATGAGATTGGTGGCCCAGCTTTCCGAATCATG
  TGGGCTGTTCATAATGGTACTCGACCACCACTGATAAAAAATTTACCTAAGCCCATTGAG
  AGCCTGATGACTCGTTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATT
  GTGAAAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTACAGTAT
  CCTTGTCAGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAGTACAGGCTCATTCATG
  GACATTGCTTCTACAAATACGAGTAACAAAAGTGACACTAATATGGAGCAAGTTCCTGCC
  ACAAATGATACTATTAAGCGCTTAGAATCAAAATTGTTGAAAAATCAGGCAAAGCAACAG
  AGTGAATCTGGACGTTTAAGCTTGGGAGCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCC
  CCAACCTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGGATCGCC
  GCAACCACAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGACTTGACTGTAACTGGA
  ACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCAGTCCCAGTGTCAGAATGATTACTACC
  TCAGGACCAACCTCAGAAAAGCCAACTCGAAGTCATCCATGGACCCCTGATGATTCCACA
  GATACCAATGGATCAGATAACTCCATCCCAATGGCTTATCTTACACTGGATCACCAACTA
  CAGCCTCTAGCACCGTGCCCAAACTCCAAAGAATCTATGGCAGTGTTTGAACAGCATTGT
  AAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGCATTGTTATTACAGAGAAAG
  CAAGAACTAGTTGCAGAACTGGACCAGGATGAAAAGGACCAGCAAAATACATCTCGCCTG
  GTACAGGAACATAAAAAGCTTTTAGATGAAAACAAAAGCCTTTCTACTTACTACCAGCAA
  TGCAAAAAACAACTAGAGGTCATCAGAAGTCAGCAGCAGAAACGACAAGGCACTTCATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003188 unedited
  CCCGGTCAGATTTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGGCTGGAG
  CCAGAAGCCGGACACGGCTGTGGCCGCTGCCTCTACCCCCGCCACGGATCGCCGGGTAGT
  AGGACTGCGCGGCTCCAGGCTGAGGGTCGGTCCGGAGGCGGGTGGGCGCGGGTCTCACCC
  GGATTGTCCGGGTGGCACCGTTCCCGGCCCCACCGGGCGCCGCGAGGGATCATGTCTACA
  GCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGCCGGTGAGATGATCGAAGCCCCTTCC
  CAGGTCCTCAACTTTGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGA
  AGAGGAGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAA
  CAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTATCCCGT
  GTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAATCCAGTGTGTCTTGTG
  ATGGAATATGCTGAAGGGGGCTCTTTATATAATGTGCTGCATGGTGCTGAACCATTGCCA
  TATTATACTGCTGCCCACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTAT
  CTTCACAGCATGCAACCCAGAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTG
  CTGGTTGCAGGNGGGACAGTTCTAAAAATTTGTGATTTTGGTACAGCCTGTGACATTCAG
  ACACACATGACCAATACCAGGGGGAGTGCTGCTTGGATGGCACCTGAGTTTTTGAGGTAG
  TATTACAGTGAAAATGTGACGTCTTCAGCTGGGTATTATTCTTTGGGAGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003188 unedited
  CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCATGTTCTTTTGCCTT
  TTAATTTCATTTAAGAGATTAATATTAGAGTCATAAAAGCTTTACAGTTTCAACACACAT
  ACACACAATCACAGAATTAAAAATCTTTACTCCTTAAAAAAAAAATGAGTTCCATTTTGT
  TCAATGTATCACAATCAAATTTTAGTCTAACAAAATCCGGTAAAACTGCTTTCATTAAAT
  AGAGCAGCTGCCACTTACCTTTACATATTTTACGGATGGAACTGAGTTCAGTACTGATCC
  AAGGATGCTGGCATCAAAGCCCTTACACGGAACTATCCCTAAAGGTTCCGTATTTCCGTA
  ATATACCACCAATCACTCTAAATTTATAATACCCTGTCTTTAACTTGGTATATACCATCT
  TTTGGGAAAAAAATTATTAATATTTTGAGATGAGATACAAGAATAAAGTCATTCTTGAGG
  TTCCACCTCTAATATGAAAAAATGGACGCTGTTTGCACATAGCAGCTAGTTTGATACCTC
  CTGTTCTGTTAGGCTAGCCTTCACACAATGAGCATGCATATACAGCCACAGTTCACTGCA
  TCTGTTTTGAAGTTGCAAAGTGCTGCTCATTCAAGTCACAGATGCTACCATGTTATGCAA
  TGAAACAGTAAAATTGTATGTCCACCATGAGAAAAGGAAAATAACAATGAAAAAAATGCC
  GCAAATATAGGGCAGTGGCATTCAGAACACGCCCAAAACCTAACACTCATGAATCGGCAT
  TAAAGGCTTTTCCTTTCCCTTAAAAAAAGTCTTTTCTTTGCATATTCAAATTTAACCGTC
  CCCGAGAATCATGAGTGGCCTGCGGTTCTGCTGCTGACTTTGGAGACCTCTATTGGTTTT
  TGCATGGCGGGAAAAAGAAAAAGCTTTTGTTTCATCTAAGCCTTTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003188
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003188.2, NP_003179.1
• RefSeq Size: 2912 bp
• RefSeq ORF: 1740 bp
• Locus ID: 6885
• UniProt ID: O43318
• Cytogenetics: 6q15
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, MAPK signaling pathway, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (A) lacks an in-frame coding segment, compared to variant B. The resulting isoform (A) lacks an internal region, as compared to isoform B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.