SYK (NM_003177) Human Untagged Clone

CAT#: SC118115

SYK (untagged)-Human spleen tyrosine kinase (SYK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SYK
• Synonyms: IMD82; p72-Syk
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003177 edited
  ATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATC
  ACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTG
  CTGCGCCAGAGCCGCAACTACCTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAG
  GCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGG
  ACCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTC
  TGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTT
  GAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCAGGGT
  CAGGCTCTGGAGCAGGCCATCATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACC
  ACAGCCCATGAAAAAATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAA
  ATTGTCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGACAACAAC
  GGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCACTATCGCATCGACAAA
  GACAAGACAGGGAAGCTCTCCATCCCCGAGGGAAAGAAGTTCGACACGCTCTGGCAGCTA
  GTCGAGCATTATTCTTATAAAGCAGATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAA
  AAAATCGGCACACAGGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCAT
  CCTGCGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTCCCAAAG
  CCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCAAGAGAGTACTGTGTCATTC
  AATCCGTATGAGCCAGAACTTGCACCCTGGGCTGCAGACAAAGGCCCCCAGAGAGAAGCC
  CTACCCATGGACACAGAGGTGTACGAGAGCCCCTACGCGGACCCCGAGGAGATCAGGCCC
  AAGGAGGTTTACCTGGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGT
  AATTTTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACCGTGGCT
  GTGAAAATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGATGAGTTATTAGCAGAA
  GCAAATGTCATGCAGCAGCTGGACAACCCGTACATCGTGCGGATGATCGGGATATGCGAG
  GCCGAGTCCTGGATGCTGGTTATGGAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTG
  CAGCAGAACAGACATGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATG
  GGCATGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGAAATGTG
  TTGCTAGTTACCCAACATTACGCCAAGATCAGTGATTTCGGACTTTCCAAAGCACTGCGT
  GCTGATGAAAACTACTACAAGGCCCAGACCCATGGAAAGTGGCCTGTCAAGTGGTACGCT
  CCGGAATGCATCAACTACTACAAGTTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTG
  TTGATGTGGGAAGCATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAA
  GTCACCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCAAGAGAG
  ATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGAAAACAGGCCCGGATTCGCA
  GCAGTGGAACTGCGGCTGCGCAATTACTACTATGACGTGGTGAACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003177 unedited
  ACCTGTTTTGTAACCGACTTCATATAGGCGGCACCGCAATTCGCACGAGGCGGGGAACGA
  CGCGCTGCTTTCATGCCCTTTCTTGTTCTACCTTCATCGGCCGAGGTAAAAGTGCTGAAA
  CCATGTGAATAAAATACAGGTGGACACCTGCGCAGGTGTGTGCCCTCCGGCCCCTGAAGC
  ATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTTCTTTTTCGGCAACATC
  ACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGGGGGGCATGAGTGATGGGCTTTATTTG
  CTGCGCCAGAGCCGCAACTACCTGGGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAG
  GCACACCACTACACCATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGG
  ACCCATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGCCTGGTC
  TGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCCCAAGACTGGGCCCTTT
  GAGGATTTGAAGGAAAACCTCATCAGGGAATATGTGAAGCAGACATGGAACCTGCANGGT
  CAGGCTCTGGAGCATGCCATCATCAGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACC
  ACAGCCCATGAAAAAATGCCTTGGTTCCATGGATAAATCTCTCGGGAAGAATCTGAGCAA
  ATTGTCCTGATAGGATCATAGACAAATGGNAAGTCCTGATCCGAGCCAGAGACAACAACG
  GCTCCTACGCCCTGTGCCTGCTGCACGATGGGAATGTGCTGCACTATCGCATCGACAAAG
  ACAAGACAGGGAAGCTCTCCATCCCCCGAGGGTATGAAGTTCGACACGCTCTGGCAACTA
  GTCGAGCCATTATTCTATAAAGCAGATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003177 unedited
  TGGGTCGAGTTTTATTACGTCCCCCTTTCTTTCTTTCTTTCCTTAACTGGCTACATGCTT
  AGAAACTGCCCCGGTCAAACTTGATTTTCTTTTTAAAGCCTCAAAACATTTTTATTGTCA
  GGAAAGCTTTTCAGGGGCCAGGGATCAGTCTCATGGCCGTAGAAGCAGCCAAATTCCTCT
  GCCTTTGCCTTCCCGGCGTGAGTCACATGCTAAGGCATCCTTGGGCATTTGGAAAAGGCC
  GCTTGGGGTGAGAGTGCTCTAGGCCACTCTGCAATGTCCCTGGGCCCGATGAGTAACAAA
  TGCACCCTGGGACCCAGAGAAGTGGAAAGACATGAAAGGGACTTGGAAACAGATCGTAAA
  AATAACCTGTATGAAAATCACACAGACAAGAAAACAAACAAATCCAGCTAGCCCCTTGGA
  TCCTGCCATCCGTCTTTTCTCCCGGGACTGCCTTTGCTTTGTGGAGGGCGAGCCCTGNGA
  CAGGCTTTGGGTGACAAGTTGTGGGCATGTTCCATCCAAGCCTGGCTCTCCCGACTGGCA
  GAGGGACGCGGCTGACAATCAAACCCTCTGGATACATTTACCTGTGATGGCTCCTCGATC
  AAAGGCAGCCCCCGACAGGGGCCGGAGCCGATACGTCACCACGCAATAGAAGAAAACGCG
  CCACCCGAAGACCACTGGGGGAATCCCGCGCTGTCTTCCCACCGGTGGACAGCCCAAATC
  AGGAAACCGACACTCTCCTTGAAACCCTGAAGGACACCCCCCCGCCACCTTGCCCAAAAA
  CGGCGGACGACTTGCCTGCACCCCACCCGCCCAGGGCCAAAGGAAAGGGACCCAATACCC
  CCCAAAGCCGCCCCATACCAACGGGGGAAACTTGAAAATTCGGAGTCTCCAGGCACGACC
  ATTGAAAGGCCCCACCCGGGACGGGCCCCCGCCGCCGCCCCCACCCCCGCCCCAAACATC
  CAAACTGCACCCGCGCAGGGGCCCACAACAAACAACGCGGCAGCGCACGGGCCGAAACGC
  CCGCGACACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003177
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003177.3, NP_003168.2
• RefSeq Size: 2672 bp
• RefSeq ORF: 1908 bp
• Locus ID: 6850
• UniProt ID: P43405
• Cytogenetics: 9q22.2
• Domains: pkinase, SH2, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity
• Gene Summary: This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (Syk(L)). Both variants 1 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.