Ceramide glucosyltransferase (UGCG) (NM_003358) Human Untagged Clone

CAT#: SC118052

UGCG (untagged)-Human UDP-glucose ceramide glucosyltransferase (UGCG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ceramide glucosyltransferase
• Synonyms: GCS; GLCT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118052 sequence for NM_003358 edited (data generated by NextGen Sequencing)
  ATGGCGCTGCTGGACCTGGCCTTGGAGGGAATGGCCGTCTTCGGGTTCGTCCTCTTCTTG
  GTGCTGTGGCTGATGCATTTCATGGCTATCATCTACACCCGATTACACCTCAACAAGAAG
  GCAACTGACAAACAGCCTTATAGCAAGCTCCCAGGTGTCTCTCTTCTGAAACCACTGAAA
  GGGGTAGATCCTAACTTAATCAACAACCTGGAAACATTCTTTGAATTGGATTATCCCAAA
  TATGAAGTGCTCCTTTGTGTACAAGATCATGATGATCCAGCCATTGATGTATGTAAGAAG
  CTTCTTGGAAAATATCCAAATGTTGATGCTAGATTGTTTATAGGTGGCAAAAAAGTTGGC
  ATTAATCCTAAAATTAATAATTTAATGCCAGGATATGAAGTTGCAAAGTATGATCTTATA
  TGGATTTGTGATAGTGGAATAAGAGTAATTCCAGATACGCTTACTGACATGGTGAATCAA
  ATGACAGAAAAAGTAGGCTTGGTTCACGGGCTGCCTTACGTAGCAGACAGACAGGGCTTT
  GCTGCCACCTTAGAGCAGGTATATTTTGGAACTTCACATCCAAGATACTATATCTCTGCC
  AATGTAACTGGTTTCAAATGTGTGACAGGAATGTCTTGTTTAATGAGAAAAGATGTGTTG
  GATCAAGCAGGAGGACTTATAGCTTTTGCTCAGTACATTGCCGAAGATTACTTTATGGCC
  AAAGCGATAGCTGACCGAGGTTGGAGGTTTGCAATGTCCACTCAAGTTGCAATGCAAAAC
  TCTGGCTCATATTCAATTTCTCAGTTTCAATCCAGAATGATCAGGTGGACCAAACTACGA
  ATTAACATGCTTCCTGCTACAATAATTTGTGAGCCAATTTCAGAATGCTTTGTTGCCAGT
  TTAATTATTGGATGGGCAGCCCACCATGTGTTCAGATGGGATATTATGGTATTTTTCATG
  TGTCATTGCCTGGCATGGTTTATATTTGACTACATTCAACTCAGGGGTGTCCAGGGTGGC
  ACACTGTGTTTTTCAAAACTTGATTATGCAGTCGCCTGGTTCATCCGCGAATCCATGACA
  ATATACATTTTTTTGTCTGCATTATGGGACCCAACTATAAGCTGGAGAACTGGTCGCTAC
  AGATTACGCTGTGGGGGTACAGCAGAGGAAATCCTAGATGTATAA
  
  Clone variation with respect to NM_003358.1
  348 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003358 unedited
  AGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCACCCCCCT
  CCGCCCTTTCCTCTCCCCACCTTCCTCTCGCCTCCCGCGCCCCCGCACCGGGCGCCCACC
  CTGTCCTCCTCCTGCGGGAGCGTTGTCCGTGTTGGCGGCCGCAGCGGGCCGGGCCGGTCC
  GGCGGGCCGGGGGATGGCGCTGCTGGACCTGGCCTTGGAGGGAATGGCCGTCTTCGGGTT
  CGTCCTCTTCTTGGTGCTGTGGCTGATGCATTTCATGGCTATCATCTACACCCGATTACA
  CCTCAACAAGAAGGCAACTGACAAACAGCCTTATAGCAAGCTCCCAGGTGTCTCTCTTCT
  GAAACCACTGAAAGGGGTAGATCCTAACTTAATCAACAACCTGGAAACATTCTTTGAATT
  GGATTATCCCAAATATGAAGTGCTCCTTTGTGTACAAGATCATGATGATCCAGCCATTGA
  TGTATGTAAGAAGCTTCTTGGAAAATATCCAAATGTTGATGCTAGATTGTTTATAGGTGG
  CAAAAAAGTTGGCATTAATCCTAAAATTAATAATTTAATGCCAGGATATGAAGTTGCAAA
  GTATGATCTTATATGGATTTGTGATAGTGGAATAAGAGTAATTCCAGATACGCTTACTGA
  CATGGTGAATCAAATGACAGAAAAAGTAGGCTTGGTTCACGGGCTGCCTTACGTAGCAGA
  CAGACAGGGCTTTGCTGCCACCTTAGAGCANGTATATTNTGGAACTTCACATCCAAGATA
  CTATATCTCTGCCAATGTAACTGGTTTCAAATGTGTGACAGGAAATGTCTGTTTTAATGA
  GAAAGATGTGTTGGNATCAAGCAGGAGGACTTTATAGCTTTTGCTCAGTACATTGCCCGA
  AGATACTTTATGGCCNAGCGATAGCTGACCGAGGTTGGAGGTTNGCATGTCNCACTCAGT
  GCATGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003358 unedited
  GCACGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTGTTTGTTTCTGCAAGGCCTA
  GCTATCTTATTACAATTTATATTTGCTCTGCAACAACTACAAGAGGTAGGCATAAAGCAT
  GAAAAAGTTTCTCAGTTAGTACCTCGCCATGTGTTGTTCACTGCACAAAACCATTCACTT
  TGGACATGAATAGTCATTCCTTCTATTATCTTCTAAACTCTAAAGCAAAGCTGCTGCCAC
  CCGTAAATGTTCTAAAAGAGTGTACTCACAAGTAAGAAACTTTCTCTACTGAAGGATACT
  GTCACAGAGTTTGTTGCAGAGCATCTATATATATATTTATTTATTTATTTTAAAAAAATA
  AACAACAATGATGAACGAACCCAGGTTCCTAGAACCAATTCTCTTGATTCTCTACTTCCA
  CAAAATAAAGTGTATCATTTGGCCAAGACTACAGATGTGTTTTTTTTTTTTTTCACAGAT
  GCAAGTGCCATGCAAAAATAAATTAAAGAACAGATCCCAAAACATACATGTGATAAAACT
  ACAGAAGGTAGATTTTTAAAAGCATTTATATAAACATAATTTATAATACTTCTCTTTTTT
  CCTTTATATACAGTCACAAAGCTGTAGTTATACATCTAGGATTTCCTCTGCTGTACCCCC
  ACAGCGTAATCTGTAGCGACCAGTTCTCCAGCTTATAGTTGGGTCCCATAATGCAGACAA
  AAAAATGTATATTGTCATGGATTCGCGGATGAACCANGCGACTGCATAATCAAGTTTGAA
  AAAACAGTGTGCACCCTGGACACCCTGAGTTGATGTGGCAAAATAAACCTGCCCGCCATG
  ACCCTGAAAATACCTATTTCCCTCTGACCCTGGGGGCTGCCATCCATATTAAACTGGCAC
  AAGCATCCGAATTGGCTCACAATTATGACCAGAAACATGTATCCGANTTGGCCCACCGAT
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003358
• Insert Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003358.1, NP_003349.1
• RefSeq Size: 1637 bp
• RefSeq ORF: 1185 bp
• Locus ID: 7357
• UniProt ID: Q16739
• Cytogenetics: 9q31.3
• Domains: Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014]