UBE2L3 (NM_003347) Human Untagged Clone

CAT#: SC118049

UBE2L3 (untagged)-Human ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2L3
• Synonyms: E2-F1; L-UBC; UBCH7; UbcM4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003347 edited
  ATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAA
  AACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTT
  CCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAG
  TACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGAC
  GAAAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAA
  ACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCG
  CTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCT
  GAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAAAATCTGCCACGATTG
  GTTCCAGCAAGTGTGAGCAGAGACCCCGTGCAGTGCATTCAGACACCCCGCAAAGCAGGA
  CTCTGTGGAAATTGACACGTGCCACCGCCTGGCGTTCGCTTGTGGCAGTTACTAACTTTC
  TACAGTTTTCTTAATCAAAAGTGGTCTAGGTAACCTGTAAAGAAAGGATTAAAAATTTAA
  GATGTTCTAGTTCTGCTCTCTTTGTTTTAAAAATCACTGCTTCAATCTACTTCAAAAGAA
  TGGTGTTTCTTTTCTTGTCCAATTTTATCCAAAATCTTCAAGTTACATTTAACCCATAAG
  GTTTAAAAAAAAAAAAAAAAAACTCGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003347 unedited
  TTTTCACCATTTTGTCATACGATTTACTATAGGCGGCCGCGAAATTCGCACGAGGCCAAG
  ATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAACCGCAAAGTGGGATGAAAAA
  CTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCC
  TGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTA
  CCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGA
  AAAGGGGCAGGTCTGTCTGCCAGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAAC
  CGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGCACCCGCT
  TCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGA
  AGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAAAATCTGCCACGATTGGT
  TCCAGCAAGTGTGAGCAGAGACCCCGTGCAGTGCATTCAGACACCCCGCANAGCATGACT
  CTGTGGAAATTGACACGTGCCACCGCCTGGCGTTCGCTTTGTGGCAGTTACTAACTTTCT
  ACAGTTTTCTTAATCAAAAGTGGTCTATGTAACCTGTAAAGAAAGGATTAAAAATTAAAG
  ATGTTCTAGTTTCTGCTCTCTTTGTTTTAAAATCACTGCTCCAATCTACTTCAAAAGATG
  GTGTTTCTTTTTTTTGTCCAATTTATCCAAAATCTTTAAGTTACATTTTAACCCATAAAG
  GTTTAAAAAAATAAAAAAAAAAACTCGCACTTTTAAATTGCGGCCCGGGGTCATATACTG
  GTTTCCTTGAAAGAATCTCCGGTGGTCTATTTCTGNGAACCCCTTCCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003347 unedited
  CCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAAACCTTATGGGTTAAATGTAACT
  TGAAGATTTTGGATAAAATTGGACAAGAAAAGAAACACCATTCTTTTGAAGTAGATTGAA
  GCAGTGATTTTTAAAACAAAGAGAGCAGAACTAGAACATCTTAAATTTTTAATCCTTTCT
  TTACAGGTTACCTAGACCACTTTTGATTAAGAAAACTGTAGAAAGTTAGTAACTGCCACA
  AGCGAACGCCAGGCGGTGGCACGTGTCAATTTCCACAGAGTCCTGCTTTGCGGGGTGTCT
  GAATGCACTGCACGGGGTCTCTGCTCACACTTGCTGGAACCAATCGTGGCAGATTTTAGT
  CCACAGGTCGCTTTTCCCCATATTTCTTTGTAAACTCTTCAGCATTCTTACAGAATTTTT
  TACGGTCCTTAGAGTATTCTTCAGCTAGGTCAGCCCGAAGCGGGTGCTCAGGCTGGGGGT
  CATTCACCAGTGCTATGAGGGACTGGATTACTTGGTCGGTTTTGGTTGCTGGCTTCCAGT
  TTTCGGCACTAATTACTGGCAGACAGACCTGCCCCTTTTCGTCGATGTTTGAGTGATAGA
  TCTTTGTTCTAATGTGATCTTCGGAGGTTTGAATGGGTACTCTGCTGAAAAGTGATTTCG
  ATTCTGAAGGCTCCCTTANATATGGAGGCNTGCAGGAACAATAGCCCCTGCCAGTCATAA
  ATTAGCTCATCAACCTGGAGTTACGGAAGTTTTTATCCACATTTGCGGATTTTTAAGCTC
  CTTATAGCCTCCTGTGCCCGCTCTGGGCTTTGCCGATTCGCGGCGGCTTTGGAGGCGATT
  ACAAATCTGAGGTTACTAACCAGCTTGCTTTTTGGACTCCACCGACCGCCTAACGGCATT
  GGTAACGGGGCGGGGATTACAATTTTGGAAGCCGCGTGTTTGGGCCAACAAATCCTTGCG
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003347
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003347.2, NP_003338.1
• RefSeq Size: 2907 bp
• RefSeq ORF: 465 bp
• Locus ID: 7332
• UniProt ID: P68036
• Cytogenetics: 22q11.21
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) uses an alternate 5' splice site and downstream translational start site, compared to variant 4. It encodes a shorter and distinct N-terminus in isoform 1, compared to isoform 4.