Mps1 (TTK) (NM_003318) Human Untagged Clone

SKU SC118032

TTK (untagged)-Human TTK protein kinase (TTK), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Mps1
• Synonyms: CT96; ESK; MPH1; MPS1; MPS1L1; PYT
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003318 edited; ATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGATTCCATAATGAACAAAGTG; AGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGATGAACTAAGCTTGAATAAA; ATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAAATTATGATGATGGCAAAC; AACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAAAACAGTGTTCCGCTAAGT; GATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCAATTGAAGCGCTTCCCCCA; GATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTGAGATTTGCTGAATTAAAA; GCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATGGCCAGAGCAAACTGCAAG; AAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTGTCACAAGGTAATGTCAAA; AAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCAGTACCACTAGAAATGCTG; GAAATTGCCCTGCGGAATTTAAACCTCCAAAAAAAGCAGCTGCTTTCAGAGGAGGAAAAG; AAGAATTTATCAGCATCTACGGTATTAACTGCCCAAGAATCATTTTCCGGTTCACTTGGG; CATTTACAGAATAGGAACAACAGTTGTGATTCCAGAGGACAGACTACTAAAGCCAGGTTT; TTATATGGAGAGAACATGCCACCACAAGATGCAGAAATAGGTTACCGGAATTCATTGAGA; CAAACTAACAAAACTAAACAGTCATGCCCATTTGGAAGAGTCCCAGTTAACCTTCTAAAT; AGCCCAGATTGTGATGTGAAGACAGATGATTCAGTTGTACCTTGTTTTATGAAAAGACAA; ACCTCTAGATCAGAATGCCGAGATTTGGTTGTGCCTGGATCTAAACCAAGTGGAAATGAT; TCCTGTGAATTAAGAAATTTAAAGTCTGTTCAAAATAGTCATTTCAAGGAACCTCTGGTG; TCAGATGAAAAGAGTTCTGAACTTATTATTACTGATTCAATAACCCTGAAGAATAAAACG; GAATCAAGTCTTCTAGCTAAATTAGAAGAAACTAAAGAGTATCAAGAACCAGAGGTTCCA; GAGAGTAACCAGAAACAGTGGCAATCTAAGAGAAAGTCAGAGTGTATTAACCAGAATCCT; GCTGCATCTTCAAATCACTGGCAGATTCCGGAGTTAGCCCGAAAAGTTAATACAGAGCAG; AAACATACCACTTTTGAGCAACCTGTCTTTTCAGTTTCAAAACAGTCACCACCAATATCA; ACATCTAAATGGTTTGACCCAAAATCTATTTGTAAGACACCAAGCAGCAATACCTTGGAT; GATTACATGAGCTGTTTTAGAACTCCAGTTGTAAAGAATGACTTTCCACCTGCTTGTCAG; TTGTCAACACCTTATGGCCAACCTGCCTGTTTCCAGCAGCAACAGCATCAAATACTTGCC; ACTCCACTTCAAAATTTACAGGTTTTAGCATCTTCTTCAGCAAATGAATGCATTTCGGTT; AAAGGAAGAATTTATTCCATATTAAAGCAGATAGGAAGTGGAGGTTCAAGCAAGGTATTT; CAGGTGTTAAATGAAAAGAAACAGATATATGCTATAAAATATGTGAACTTAGAAGAAGCA; GATAACCAAACTCTTGATAGTTACCGGAACGAAATAGCTTATTTGAATAAACTACAACAA; CACAGTGATAAGATCATCCGACTTTATGATTATGAAATCACGGACCAGTACATCTACATG; GTAATGGAGTGTGGAAATATTGATCTTAATAGTTGGCTTAAAAAGAAAAAATCCATTGAT; CCATGGGAACGCAAGAGTTACTGGAAAAATATGTTAGAGGCAGTTCACACAATCCATCAA; CATGGCATTGTTCACAGTGATCTTAAACCAGCTAACTTTCTGATAGTTGATGGAATGCTA; AAGCTAATTGATTTTGGGATTGCAAACCAAATGCAACCAGATACAACAAGTGTTGTTAAA; GATTCTCAGGTTGGCACAGTTAATTATATGCCACCAGAAGCAATCAAAGATATGTCTTCC; TCCAGAGAGAATGGGAAATCTAAGTCAAAGATAAGCCCCAAAAGTGATGTTTGGTCCTTA; GGATGTATTTTGTACTATATGACTTACGGGAAAACACCATTTCAGCAGATAATTAATCAG; ATTTCTAAATTACATGCCATAATTGATCCTAATCATGAAATTGAATTTCCCGATATTCCA; GAGAAAGATCTTCAAGATGTGTTAAAGTGTTGTTTAAAAAGGGACCCAAAACAGAGGATA; TCCATTCCTGAGCTCCTGGCTCATCCATATGTTCAAATTCAAACTCATCCAGTTAACCAA; ATGGCCAAGGGAACCACTGAAGAAATGAAATATGTTCTGGGCCAACTTGTTGGTCTGAAT; TCTCCTAACTCCATTTTGAAAGCTGCTAAAACTTTATATGAACACTATAGTGGTGGTGAA; AGTCATAATTCTTCATCCTCCAAGACTTTTGAAAAAAAAAGGGGAAAAAAATGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003318 unedited; TTTCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA; GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA; ATCGGCACGAGGGTAGAAATGGAATCCGAGGATTTAAGTGGCAGAGAATTGACAATTGAT; TCCATAATGAACAAAGTGAGAGACATTAAAAATAAGTTTAAAAATGAAGACCTTACTGAT; GAACTAAGCTTGAATAAAATTTCTGCTGATACTACAGATAACTCGGGAACTGTTAACCAA; ATTATGATGATGGCAAACAACCCAGAGGACTGGTTGAGTTTGTTGCTCAAACTAGAGAAA; AACAGTGTTCCGCTAAGTGATGCTCTTTTAAATAAATTGATTGGTCGTTACAGTCAAGCA; ATTGAAGCGCTTCCCCCAGATAAATATGGCCAAAATGAGAGTTTTGCTAGAATTCAAGTG; AGATTTGCTGAATTAAAAGCTATTCAAGAGCCAGATGATGCACGTGACTACTTTCAAATG; GCCAGAGCAAACTGCAAGAAATTTGCTTTTGTTCATATATCTTTTGCACAATTTGAACTG; TCACAAGGTAATGTCAAAAAAAGTAAACAACTTCTTCAAAAAGCTGTAGAACGTGGAGCA; GTACCACTAGAAATGCTGGNAATTGCCCCTGCGAATTANACCTNCAAAAAAGCAGCTGCT; TTCAGAGGAGGAAAAGAAAGATTTATCAGCATCTACGGTATTAACTGCCCCAGATCATTT; TCCGGGTCACTTGGGCATTTACAGAATANGAACAACAGTTGTGAATTCAGAGGACAGACT; ACTAANGCCAGNTTTTATATGGGAGAGACATGCCANNCACAGAGCAGAAATAGGNTACCG; GNAATTCATTTGAGACAACTANNCAAAACTAACAGTCATGCCCATTTTGGNNAGNAGTCC; NAGNTNACCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003318 unedited; AATTGGGTCCCAAACTTTTTTACAAATTTTTTAAAACTACTTGCTATCCACCCACTATTC; CAANAGTAAAATGATGTCAAGTAGATTACACAAGAGCATAAAACAGAGAAAACAAGTCTA; CAATATATACAGTGCCATAAGTGGTTGCTATTTTTACAGTTTTCTTTTAAAGATAATCTA; CTGAATTTTTTTTGCTGATAACACTTTATAGTGATGTTGTCTTCAAATGTAGATTTCCAC; AGGGATTCAAGAGTATAACAGTCCAATATATTTTATAGGTGGTATCTGACATTACGAATA; ACTGCAAATCATTTTTTTCCCCTTTTTTTTTCAAAAGTCTTGGAGGATGAAGAATTATGA; CTTTCACCACCACTATAGTGTTCATATAAAGTTTTAGCAGCTTTCAAAATGGAGTTAGGA; GAATTCAGACCAACAAGTTGGCCCAGAACATATTTCATTTCTTCAGTGGTTCCCTTGGCC; ATTTGGTTAACTGGATGAGTTTGAATTTGAACATATGGATGAGCCAGGAGCTCAGGAATG; GATATCCTCTGTTTTGGGTCCCTTTTTAAACAACACTTTAACACATCTTTGAGATCTTTC; TCTGGAAATATCGGAAANTTCATTTCATGATTAGGATCAATTATGGCATGTAATTTAGAA; ATCTGATTAATTATCTGCTGAAATGGTGTTTTCCNGTNAGTCATATAGTACAAAATACAT; TCTAAGGACCAAACATCACTTTTTGGGGCTTATTCTTTGACTTTAGATTTTCCATTTCTC; TCCTGGAGGAAGACATAATCTTTGATTGCTTCTGGTGGCATATAATAACTGGGCCAACCT; GAAAAATTTTTACAACCCTTGTTGATCCTGTTGCATTTGGGTTGCATCCCCAAATCAATA; GCTTTAGCATTCCTCAACTTCAGAAAGTACCTGGTTTAGAACCTGGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003318
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003318.3, NP_003309.2
• RefSeq Size: 2984 bp
• RefSeq ORF: 2574 bp
• Locus ID: 7272
• UniProt ID: P33981
• Cytogenetics: 6q14.1
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway
• Summary: This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).