DEK (NM_003472) Human Untagged Clone

CAT#: SC117956

DEK (untagged)-Human DEK oncogene (DEK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DEK
• Synonyms: D6S231E
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003472, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGCCTCGGCCCCTGCTGCGGAGGGGGAGGGAACCCCCACCCAGCCCGCGTCCGAGAAAGAACCCG
  AAATGCCCGGTCCCAGAGAGGAGAGCGAGGAGGAAGAGGACGAGGACGACGAGGAGGAGGAGGAGGAGGA
  AAAAGAAAAGAGTCTCATCGTGGAAGGCAAGAGGGAAAAGAAAAAAGTAGAGAGGTTGACAATGCAAGTC
  TCTTCCTTACAGAGAGAGCCATTTACAATTGCACAAGGAAAGGGGCAGAAACTTTGTGAAATTGAGAGGA
  TACATTTTTTTCTAAGTAAGAAGAAAACCGATGAACTTAGAAATCTACACAAACTGCTTTACAACAGGCC
  AGGCACTGTGTCCTCATTAAAGAAGAATGTGGGTCAGTTCAGTGGCTTTCCATTTGAAAAAGGAAGTGTC
  CAATATAAAAAGAAGGAAGAAATGTTGAAAAAATTTAGAAATGCCATGTTAAAGAGCATCTGTGAGGTTC
  TTGATTTGGAGAGATCAGGTGTAAATAGTGAACTAGTGAAGAGGATCTTGAATTTCTTAATGCATCCAAA
  GCCTTCTGGCAAACCATTGCCGAAATCTAAAAAAACTTGTAGCAAAGGCAGTAAAAAGGAACGGAACAGT
  TCTGGAATGGCAAGGAAGGCTAAGCGAACCAAATGTCCTGAAATTCTGTCAGATGAATCTAGTAGTGATG
  AAGATGAAAAGAAAAACAAGGAAGAGTCTTCAGATGATGAAGATAAAGAAAGTGAAGAGGAGCCACCAAA
  AAAGACAGCCAAAAGAGAAAAACCTAAACAGAAAGCTACTTCTAAAAGTAAAAAATCTGTGAAAAGTGCC
  AATGTTAAGAAAGCAGATAGCAGCACCACCAAGAAGAATCAAAACAGTTCCAAAAAAGAAAGTGAGTCTG
  AGGATAGTTCAGATGATGAACCTTTAATTAAAAAGTTGAAGAAACCCCCTACAGATGAAGAGTTAAAGGA
  AACAATAAAGAAATTACTGGCCAGTGCTAACTTGGAAGAAGTCACAATGAAACAGATTTGCAAAAAGGTC
  TATGAAAATTATCCTACTTATGATTTAACTGAAAGAAAAGATTTCATAAAAACAACTGTAAAAGAGCTAA
  TTTCTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003472 unedited
  TTCGAATTTTGTAATACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGCGCGTGCC
  AGGCCCGCGGCGGCCGAAATCCGCGGTTCACAGCATGTCCGCCTCGGCCCCTGCTGCGGA
  GGGGGAGGGAACCCCCACCCAGCCCGCGTCCGAGAAAGAACCCGAAATGCCCGGTCCCAG
  AGAGGAGAGCGAGGAGGAAGAGGACGAGGACGACGAGGAGGAGGAGGAGGAGGAAAAAGA
  AAAGAGTCTCATCGTGGAAGGCAAGAGGGAAAAGAAAAAAGTAGAGAGGTTGACAATGCA
  AGTCTCTTCCTTACAGAGAGAGCCATTTACAATTGCACAAGGAAAGGGGCAGAAACTTTG
  TGAAATTGAGAGGATACATTTTTTTCTAAGTAAGAAGAAAACCGATGAACTTAGAAATCT
  ACACAAACTGCTTTACAACAGGCCAGGCACTGTGTCCTCATTAAAGAAGAATGTGGGTCA
  GTTCAGTGGCTTTCCATTTGAAAAAGGAAGTGTCCAATATAAAAAGACCGAAGACATGCT
  GAAAAAATTTAGAAATGCCCATGTTAAAGAGCATCTGTGAGGTTCTTGATTTGGAGAGAT
  CAGGTGCTAATAGTGAACTAGTGAAGAGGATCTTGAATTTCTTAATGCATCCAAAAGCCT
  TCTGGCAAACCATTGCCGAAATCCTAAAAAAACTTTGTAGCACAGGCAGTAAAAAGGAAC
  GGACCAGTTCTGGAATGGCAGGAAAGCCTAGCGAACCACATGTCCTGAAATTCTGTCAGA
  TGAATCTAGTAGTGATGAAGATGAAAAAAAAACACGCAAGAGTCTTCCAATGATGAAGAT
  CAAGAACGTGAAAGGAGCCCCCCAAAAAGACAGCCCAAGCAGAAAACCTTAACAGCAACT
  TACTTTTAAATTAAAAATCTCGTGAAGCGCCCATGTTAACAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003472 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGATTTAGATTTATTT
  TTATTGACAAGGTTTATAAGAACAAATATTTAAAATCGAAGGCCAATTATTAGGTCTCAT
  TTAGTTGCTTATTTTGTTCACTTGTATTTACCTTTCCCTAGTGTCTGAGTAACTATCAAG
  AAACAAACCTGTGAAAATACCTGTTAACATTCAACATATATTTTTATATATTTCTGTTCT
  ATGATGCAAAGATATTTTTCAACACTTAATTGGTGCAACAAATGTGTCATTGTGTCATAA
  ACAGCATGTTTTAAAATTCAGATTTAATAAACTGATTTAAGACAGTAAATTTGAAAGACA
  AAATTAAGTCTCATTCAGGAGTGGTCCATTATGTTGATCATCTAGAATCAACACTGATTA
  ACCAAACTCTGAAAGCCAAGAGCCCCAACTCCAGAGAAACATTAAATTTCTTTAATGTAA
  AAGTATATTATTTTTGAGACACACTTTGTCAGAGACATCCACGATTGCCTGCACATTATA
  TTCTGGCATTATAATCTGGTACTTTAGTCATAATCGTGAAGCTGGCTAGGTTTCCAGTAA
  ATATCAGCATTTTATATGGGCAAAAGCAGTTAATAAAAATCAGTTCATTTACTGTAAGCC
  AAATGTGCTTGTACTTAATCCCACCCTTTCCCAAAGTTTTTGAAGCTNTGATAGGTTGAT
  TTTTGGTCTGTCCTTATATATATAAAACGTACCTACAGACACTTTTACAGAGTTAATACT
  AAAATTACAATTGATGACACTTTCGAGGCAAGCANGGTAACTGGTCCTTCAGTGTTGCCT
  CACTGAATGACTTCACTGTCCATCATACTGGTTGGCTGACACTGACATTCATAGTCTACA
  CTATAAAGAACCTACCTATGTGAGTTTAAAAGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003472
• Insert Size: 3120 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003472.2, NP_003463.1
• RefSeq Size: 2879 bp
• RefSeq ORF: 1128 bp
• Locus ID: 7913
• UniProt ID: P35659
• Cytogenetics: 6p22.3
• Domains: SAP
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).