Cullin 3 (CUL3) (NM_003590) Human Untagged Clone

CAT#: SC117870

CUL3 (untagged)-Human cullin 3 (CUL3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CUL3
• Synonyms: CUL-3; NEDAUS; PHA2E
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117870 sequence for NM_003590 edited (data generated by NextGen Sequencing)
  ATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCC
  TTTCCGATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCA
  ATTCAAGAAATCCAGCGTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAAT
  GCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTACACTGGACTAAGAGAAGTT
  GTTACCGAACATCTCATAAATAAGGTGCGAGAAGATGTACTAAATTCATTGAATAACAAC
  TTTCTTCAAACGCTAAATCAAGCTTGGAATGATCATCAAACAGCTATGGTGATGATTAGA
  GACATACTAATGTACATGGACCGTGTGTATGTACAACAAAATAATGTGGAGAACGTCTAC
  AATTTGGGATTAATTATTTTTCGAGATCAAGTTGTACGTTATGGGTGTATTAGGGATCAT
  CTACGGCAAACTCTATTGGATATGATTGCAAGAGAGCGGAAAGGAGAAGTCGTAGACAGA
  GGCGCAATAAGAAATGCTTGCCAGATGTTAATGATTTTAGGTCTCGAAGGAAGATCAGTC
  TATGAAGAAGATTTTGAGGCTCCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAA
  AGCCAGAAATTTTTAGCAGAAAATAGTGCTTCAGTATATATAAAGAAAGTAGAAGCTAGA
  ATTAATGAAGAAATAGAACGAGTGATGCACTGCCTTGACAAATCAACGGAAGAACCAATT
  GTAAAGGTGGTTGAAAGGGAACTCATTTCCAAGCACATGAAGACTATAGTAGAAATGGAG
  AATTCTGGGCTAGTACATATGTTGAAAAATGGAAAGACAGAAGACCTTGGTTGCATGTAC
  AAGTTATTTAGTCGTGTGCCAAATGGTTTGAAAACAATGTGTGAGTGTATGAGTTCCTAT
  TTGAGGGAGCAAGGTAAAGCTCTTGTTTCTGAAGAAGGAGAAGGAAAGAATCCTGTTGAC
  TATATCCAGGGCTTATTGGATCTGAAGAGTAGGTTCGATCGCTTCCTCCTGGAATCATTC
  AACAATGACCGTCTCTTTAAACAAACTATTGCGGGTGACTTTGAGTATTTTCTCAACCTC
  AACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGCTGAAAAAGGGAGTC
  AAAGGGCTAACAGAACAAGAAGTAGAAACAATATTGGATAAAGCAATGGTCCTTTTTAGG
  TTTATGCAAGAAAAAGATGTATTTGAACGTTATTATAAACAACACTTGGCAAGGAGACTT
  CTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAACATGATATTTAAGTTAAAGACT
  GAATGTGGATGTCAGTTCACGTCAAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCA
  AACACAACGATGGATGAATTCAGGCAACATCTACAGGCAACTGGTGTATCTTTAGGTGGT
  GTTGATCTTACAGTCCGGGTGCTCACGACAGGATATTGGCCCACTCAGTCAGCCACACCA
  AAGTGCAACATCCCACCAGCTCCAAGACATGCTTTTGAGATATTCAGAAGGTTCTACTTA
  GCCAAACACAGTGGTCGACAGCTCACACTCCAGCATCATATGGGTTCTGCAGATCTCAAT
  GCCACATTTTATGGACCAGTTAAAAAGGAAGATGGATCTGAAGTTGGTGTTGGAGGTGCA
  CAAGTAACTGGCTCTAATACACGGAAGCACATATTGCAAGTTTCCACTTTCCAGATGACC
  ATATTAATGCTCTTTAATAATAGAGAAAAATACACATTTGAGGAAATTCAGCAAGAGACA
  GATATCCCTGAAAGAGAGCTTGTTAGAGCCCTACAGTCCCTCGCCTGTGGTAAACCAACA
  CAGCGGGTTCTTACAAAAGAACCCAAATCAAAGGAAATAGAAAATGGTCATATATTTACA
  GTTAATGATCAGTTCACATCCAAACTACACAGAGTCAAGATTCAAACAGTTGCTGCCAAA
  CAAGGTGAATCCGACCCAGAGAGGAAAGAAACAAGGCAGAAAGTAGACGACGACAGAAAA
  CATGAGATAGAAGCTGCTATAGTGCGGATAATGAAATCTAGAAAGAAGATGCAGCACAAT
  GTTCTAGTAGCGGAGGTAACTCAGCAGTTGAAGGCGCGATTCTTACCAAGTCCAGTTGTT
  ATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGAT
  CGCAAAGTATACACATATGTAGCATAA
  
  Clone variation with respect to NM_003590.3
  1367 c=>t;1581 a=>t;1992 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003590 unedited
  GCACGAGGCGGAGGAGGAGAATGAGGAGGAGGAGGACGACGTTCGGCCTGCGCAGTGAGA
  TGTTTGTCCGTCGCCGCCGCCGCCGCCATCGCGGAGGAGCGCGATAAAGGGAGCCGAGCC
  GGACGTGAGGGGGACCCCGCGGAGCCGCCGCGCCAGCGCAGCCCCCCAGCCGCATCGGAG
  TCGCCAGAGTCCGAGCCGTCGTCGCCGCCGCCCCCGCCGCCGTCGCGGCTGCCCATGGGC
  CGGCCAGCTCCCCAGCCCTGCCCGGGGGTCGCGCCGCCGCCGCCCCCAGCGTCGCCCCTA
  GCCTGCCTGCCCGCCTTAAATGTGACACCGGCGTTGCGGAGCGCGACCTGTAGCCGCCGC
  CGGGGAGGGGACTAGCACCATGTCGAATCTGAGCATAGGCACGGGCATCCGGAAGGACAC
  CAAGATGCAGATCCGGGCCTTTTCGATGACCATGGATGAAAAATATGTAAACAGCATTTG
  GGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGTAATAATAACAGTGGTCTTAGTTC
  TGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACATGGAGAAAAGCTCTA
  CACTGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003590 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAATCCACAATTTTAT
  TGTAATGAGCTGTATTTTCTAAATTTCTCTTTTATTTTCCTGTTTTCATACAGTAAAGAA
  AACAAAACGCAGCACATTCACATTTTCCCGAGGAACTGTAAAGATGATCTCTACAGGGAT
  AAACGTTGTAAAGCCTGAAGCTCAATCAACTGATGTTGGAAACTCTCAAAGGGAGTAAAG
  GCTTGATCTCAATGGTCTAGAACATGTACTGTAATTTAATAGAAGAGATGGTCGTCTTAA
  TATTTAATGATTTAAAAGAACTTCCCAGTCCATGCGAAGAGTACAGTCCAAGAATAAATC
  AAATTTCTGAACGCATTTTATGCTACATATGTGTATACTTTGCGATCCTCAGGTGTTCGT
  GCCAAATATTCTCTCTCAATAAGTCCTTCAATACGTTTCTTAATAACAACTGGACTTGGT
  AAGAATCGCGCCTTCAACTGCTGAGTTACCTCCGCTACTAGAACATTGTGCTGCATCTTC
  TTTCTAGATTTCATTATCCGCACTATAGCAGCTTCTATCTCATGTTTTCTGTCGGCGTCT
  ACTTTCTGCCTTGTTTCTTTCCTCTCTGGGTCGGATTCACCTTGTTTGGCAGCAACTGTT
  TGAATCTTGACTCTGTGTAGTTTGGATGTGAACTGATCATTACCTGTAAATATATGACCA
  TTTTCCTATTTCCTTTGATTTGGGGTCTTTTGTAAGAACCCGCTGTGTTGTTTTACCACC
  AGCGAGGGACTGTAGGNCTCTAACAGCTCCTCTTTCAGGATATCTGGCCCTTGCTGAATT
  TCCTCAAAGTGGAATTTNCTCTTTTATAAAAGAGCATAAATGGTCATCTGGAAAGGGGAA
  CTTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003590
• Insert Size: 5580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003590.2, NP_003581.1
• RefSeq Size: 6787 bp
• RefSeq ORF: 2307 bp
• Locus ID: 8452
• UniProt ID: Q13618
• Cytogenetics: 2q36.2
• Domains: CULLIN
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.