EIF3D (NM_003753) Human Untagged Clone

SKU SC117807

EIF3D (untagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EIF3D
• Synonyms: eIF3-p66; eIF3-zeta; EIF3S7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117807 sequence for NM_003753 edited (data generated by NextGen Sequencing); ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCG; GTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGA; AAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCC; TCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGGATGAAAGTAGCTTC; CAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGAATCGAATGAGATTT; GCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTTCAACCTGCAG; ATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC; CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCT; TCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATG; AAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAA; TACTACGACAAAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGCCACTGCGGAGCATC; AAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCCGCAAGCTGGCAAAA; ACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAGCTGTACCCGC; TCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG; AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAA; GATGAAGGTAATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAAC; CACAATTTCTCCCAGCAGTGCTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCA; AACCCGTTTGTGGAGGACGACATGGATAAGAATGAAATCGCCTCTGTTGCGTACCGTTAC; CGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCCGTTGTGAGCACGATGGCGTC; ATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAATGAGTGGGAT; TCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC; ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTG; CTGGCTGGATCTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCC; TCACGCCACGTCATCCTAGGCACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATC; AACCTGAGCGTGGAGAATGCCTGGGGCATTTTACGCTGCGTCATTGACATCTGCATGAAG; CTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACAAGCAGGTCATCCGTGTCTAC; AGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGAGGAGGAAGAA; GAGGAAGAAGAAGAGGAAGAAACTTAA; ; Clone variation with respect to NM_003753.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003753 unedited; GGTCACATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCAAGAGTGCT; GCTGCTAACGCGGTCCCCGGCACGCACCATCTGTTGCCATCCCGGCCGGCCGAGGCCATT; GCAGATTTTGGAAGATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCT; GGGGTCCCTGTGCGGTTCCCGAGCAGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAG; GAGATCGGCTAGGAAAGGTTGCAGACTGGACAGGAGCCACATACCAAGATAAGAGGTACA; CAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCTTATTTCCATGAGGAGG; ATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACCAGCGGA; ATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGC; AGTTCAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGAC; TGCAGAAAAAGTTCCAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGA; AACCCCGAGACTCTTCAGTTGAAGTTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATT; NTCCTCAGTTGATGAAGATGCGCTACTTGGAAGTATCAGAGCCACAGGACATTGAGTGTT; GTGGNGCCCTAGAATACTACGACANAGCCTTTGACCGCATCACCACGAGGAGTGAGAAGC; CACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTGTCATCC; CGCAGCTGGCANAACTCAGGNGAATGTGTTTGCCACTGATGCCATNCCTGCCACGCTGAT; GAGCTGTACCCGCTCAGTGTATTCTGGGAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003753 unedited; ATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTGAGGGAAA; GACTAAACAGATATATATTTTATTTCATCTGCTAAATGTCAGAGAGCAAGTTAGACACAC; ATTCCACTAAGCATCAAAGGCCCTCGTAGTCTCGGTGGAAGGACAAACTCCAGCTCCACA; TCACTGGTTTAAGTTTCTTCCTCTTCTTCTTCCTCTTCTTCCTCCTCCTCTTCCTCCTCA; TCTTCATCAGAGCTGAAGGTGCCATCAGGGAGGCTGTAGACACGGATGACCTGCTTGTTG; GGGTCCTTGAGGATGAGGTATTTGCCCTCCTCCAGCTTCATGCAGATGTCAATGACGCAG; CGTAAAATGCCCCAGGCATTCTCCACGCTCAGGTTGATCTGGCTGGCAAACTCATTAGGC; TTGAACTGCTGGGTGCCTAAGATGACGTGGCGTGAGGAGTCTTTCACGTGGTACCGAGAC; ACATAACCAAGCTTGAGGTACTCAGATCCAGCCAGCAAAGCACAGCAGGTCCACCGGGCC; AACTTGTAGCTGTTGTTCTTCAGCTCCGTGGCAATGACAGCCCCTCGCTGAGAGTCCAGC; TTCTGACGCCAGTCAACGCCATTACAGTGCCTGGAATCCCACTCATTGAGTGTCTTGATG; TTGATGAAGGACACTTCCCCGTTGGCTCCAGTCATGACGCCATCGTGCTCACAACGGACA; ATAAGGTCAATATCATCTCCAAGCTTTCACCTGCGGTAACCGTACGCAACAGAAGCGATT; TCATTCTTATCCATGTCGTCCTNCCAAACGGNTTTGGGTGGGGAAATTGTATCTTTCCTT; CCCTTCTCAACACTGCTGGGAGAATTGTGGTGATGTAGGTTGCCTCATGGCAGTTGCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003753
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003753.2, NP_003744.1
• RefSeq Size: 2169 bp
• RefSeq ORF: 1647 bp
• Locus ID: 8664
• UniProt ID: O15371
• Cytogenetics: 22q12.3
• Domains: eIF-3_zeta
• Summary: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]