VAMP8 (NM_003761) Human Untagged Clone

SKU SC117746

VAMP8 (untagged)-Human vesicle-associated membrane protein 8 (endobrevin) (VAMP8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VAMP8
• Synonyms: EDB; VAMP-8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117746 sequence for NM_003761 edited (data generated by NextGen Sequencing); ATGGAGGAAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTG; GAGGGAGTTAAGAATATTATGACCCAAAATGTGGAGCGGATCCTGGCCCGGGGGGAAAAC; TTGGAACATCTCCGCAATAAGACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACG; ACATCGCAGAAGGTGGCTCGGAAATTCTGGTGGAAGAACGTGAAGATGATTGTCCTTATC; TGCGTGATTGTTTTTATCATCATCCTCTTCATTGTGCTCTTTGCCACTGGTGCCTTCTCT; TAA; ; Clone variation with respect to NM_003761.4; 87 g=>a;138 c=>t;201 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003761 unedited; NNCCGGTTCGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTGGG; AGGAAGCCGACTAGGCGAATTCACTTACTGACCGGCCTGGGCTGCTCTGAGACATGGAGG; AAGCCAGTGAAGGTGGAGGAAATGATCGTGTGCGGAACCTGCAAAGTGAGGTGGAGGGAG; TTAAGAATATTATGACCCAAAATGTGGAGCGGATCCTGGCCCGGGGGGAAAACTTGGAAC; ATCTCCGCAATAAGACAGAGGATCTGGAAGCCACATCTGAGCACTTCAAGACGACATCGC; AGAAGGTGGCTCGGAAATTCTGGTGGAAGAACGTGAAGATGATTGTCCTTATCTGCGTGA; TTGTTTTTATCATCATCCTCTTCATTGTGCTCTTTGCCACTGGTGCCTTCTCTTAAGTAA; CAGGGAACCTCTCCCACCTGCCCTTCTTTTCAGGGACAACCCTCCATAAATGTGTGCCAA; GAGGGTCTCCTTTCCTGTCTTCCTCTACAGAGAATGCTGCTCGGTCCTCCTACCCCTCTT; CCCGAGGCCCTGCTGCCACGTTGTATGCCCCAGAAGGTACCTTGGTCCCCCGGAAGGAGA; GAAAAAAGAGAGATGGACTGTGGCTGCATTTCTTGGGTCCTTAGAGTGGGCTGGAGAGAC; CTAGAGGGCCCAGCATGTGGCTGGGAAACTGTTGGTGGCCAGTGGGTAATAAAGACCTTT; CAGTATCCCTAANAAAAAAAAAAANNNNNAAAAAANNNANAAAAAAAAAAAAAAAAAAAA; AAAACCTTCGCCTCTTAGATTGCGGNNCCGCGGGTCATAGCTGGTTTCCCTGAACAGATC; CCCGGGTGGNCATNCCNNTGTGACCCNNTNCCCCAGTGNNCCTCTCCTGGGCCCNTNGNN; AAGTTGNCCCACTCCCAGTGCCCCACCCAGCCNTTNGTNCCTAATAAAAATTAAGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003761 unedited; AACCAANTTCGAGAGNCGAGTTAAAACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTAAGGGGAACCGAAAGGGCTTTATTACCCACTGGCCACCAACAGTTT; CCCAACCACATGCTGGGCCCTTTAGGGCTTTCCAGCCCACTTTAAGGACCCAAAAAAGGC; AGCCACAGGCCATCTCTTTTTTTTTTTTTCTTCCGGGGGACCAAGGTACCTTTTGGGGCA; TACAACGGGGCAACAGGGCCTCGGGAAAAGGGGTAGGAGGACCCAACAACATTCTCTGTA; AAGGAAAACAGGAAAGGAAACCCTTTTGGCACACATTTATGGAGGGGTGGCCCTGAAAAA; AAGGGCAGGGGGGAAAGGTTCCCTGTTACCTAAAAAAAGGGACCAGGGGCAAAGAGCCCA; ATGAAGAGGGTGATGATAAAAACAATCACGCAGATAAGGACAATCATTTTCACGTTTTTC; CACCAAAATTTCCGAGCCACCTTCTGCGAAGGCCCCTTGAAGGGCTCAAAAGTGGCTTCC; AAAACCCCTGGCTTATTGGGGAGAAGTTCCAAGTTTTCCCCCCGGGCCAGGATCCGCTCC; ACATTTTGGGTCATAATATTCTTAACTCCCTCCACCTCACTTTGCAAGTTCCGCACACGA; TCATTTCCTCCACCTTCACTGGCTTCCTCCATGGCCAGAACACCCCAGGCCGTTCAATAA; GGGATATCGCCTAATCGGGTTCCTCCCAGACTCGTGCCGAAAATCGCGGCCGCCCTAAAG; AGAGTGGAATTAAAAAAATTTTGACGGTTAACTAAACGAGCTCTGGTTATTAAGACCTTC; CACCGTACACGCCTACCGCCCATTTGGGTAAACGGGGCGGGGGTAATAACAAAATTTAGA; AAAGCCCCGTGGTATTGGTGGCCAAAACAAATCCCTATGACGTAAAGGGGGGGAAACCTG; GAATACCGCGGAGGAAACACCGTTCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_003761
• Insert Size: 740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003761.2, NP_003752.2
• RefSeq Size: 702 bp
• RefSeq ORF: 303 bp
• Locus ID: 8673
• UniProt ID: Q9BV40
• Cytogenetics: 2p11.2
• Domains: synaptobrevin
• Protein Families: Stem cell - Pluripotency, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Summary: This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.[provided by RefSeq, Jun 2010]