RIP2 (RIPK2) (NM_003821) Human Untagged Clone

CAT#: SC117734

RIPK2 (untagged)-Human receptor-interacting serine-threonine kinase 2 (RIPK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIP2
• Synonyms: CARD3; CARDIAK; CCK; GIG30; RICK; RIP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003821 edited
  ATGAACGGGGAGGCCATCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGAC
  CTGCGCTACCTGAGCCGCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGG
  CGCGTCCAGGTGGCCGTGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGA
  AAGGATGTCTTAAGAGAAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCA
  ATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAAT
  GGATCATTAAATGAACTCCTACATAGGAAAACTGAATATCCTGATGTTGCTTGGCCATTG
  AGATTTCGCATCCTGCATGAAATTGCCCTTGGTGTAAATTACCTGCACAATATGACTCCT
  CCTTTACTTCATCATGACTTGAAGACTCAGAATATCTTATTGGACAATGAATTTCATGTT
  AAGATTGCAGATTTTGGTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGT
  AGCAAATCTGCACCAGAAGGAGGGACAATTATCTATATGCCACCTGAAAACTATGAACCT
  GGACAAAAATCAAGGGCCAGTATCAAGCACGATATATATAGCTATGCAGTTATCACATGG
  GAAGTGTTATCCAGAAAACAGCCTTTTGAAGATGTCACCAATCCTTTGCAGATAATGTAT
  AGTGTGTCACAAGGACATCGACCTGTTATTAATGAAGAAAGTTTGCCATATGATATACCT
  CACCGAGCACGTATGATCTCTCTAATAGAAAGTGGATGGGCACAAAATCCAGATGAAAGA
  CCATCTTTCTTAAAATGTTTAATAGAACTTGAACCAGTTTTGAGAACATTTGAAGAGATA
  ACTTTTCTTGAAGCTGTTATTCAGCTAAAGAAAACAAAGTTACAGAGTGTTTCAAGTGCC
  ATTTACCTATGTGACAAGAAGAAAATGGAATTATCTCTGAACATACCTGTAAATCATGGT
  CCACAAGAGGAATCATGTGGATCCTCTCAGCTCCATGAAAATAGTGGTTCTCCTGAAACT
  TCAAGGTCCCTGCCAGCTCCTCAAGACAATGATTTTTTATCTAGAAAAGCTCAAGACTGT
  TATTTTATGAAGCTGCATCACTGTCCTGGAAATCACAGTTGGGATAGCACCATTTCTGGA
  TCTCAAAGGGCTGCATTCTGTGATCACAAGACCACTCCATGCTCTTCAGCAATAATAAAT
  CCACTCTCAACTGCAGGAAACTCAGAACGTCTGCAGCCTGGTATAGCCCAGCAGTGGATC
  CAGAGCAAAAGGGAAGACATTGTGAACCAAATGACAGAAGCCTGCCTTAACCAGTCGCTA
  GATGCCCTTCTGTCCAGGGACTTGATCATGAAAGAGGACTATGAACTTGTTAGTACCAAG
  CCTACAAGGACCTCAAAAGTCAGACAATTACTAGACACTACTGACATCCAAGGAGAAGAA
  TTTGCCAAAGTTATAGTACAAAAATTGAAAGATAACAAACAAATGGGTCTTCAGCCTTAC
  CCGGAAATACTTGTGGTTTCTAGATCACCATCTTTAAATTTACTTCAAAATAAAAGCATG
  TAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003821 unedited
  NTTGTTCAAATTTTGNTATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGGGC
  CTCGCTCGTGCAGGGGCGTATCTGGGCGCCTGAGCGCGGCGTGGGAGCCTTGGGAGCCGC
  CGCAGCAGGGGGCACACCCGGAACCGGCCTGAGCGCCCGGGACCATGAACGGGGAGGCCA
  TCTGCAGCGCCCTGCCCACCATTCCCTACCACAAACTCGCCGACCTGCGCTACCTGAGCC
  GCGGCGCCTCTGGCACTGTGTCGTCCGCCCGCCACGCAGACTGGCGCGTCCAGGTGGCCG
  TGAAGCACCTGCACATCCACACTCCGCTGCTCGACAGTGAAAGAAAGGATGTCTTAAGAG
  AAGCTGAAATTTTACACAAAGCTAGATTTAGTTACATTCTTCCAATTTTGGGAATTTGCA
  ATGAGCCTGAATTTTTGGGAATAGTTACTGAATACATGCCAAATGGATCATTAAATGAAC
  TCCTACATAGGAAAACTGAATATCCTGATGTTGCTTGGCCATTGAGATTTCGCATCCTGC
  ATGAAATTGCCCTTGGTGTAAATTACCTGCACAATATGACTCCTCCTTTACTTCATCATG
  ACTTGAAGACTCAGAATATCTTATTGGACAATGAATTTCATGTTAAGATTGCAGATTTTG
  GTTTATCAAAGTGGCGCATGATGTCCCTCTCACAGTCACGAAGTAGCANATCTGCACCAG
  AAGGAGGGACCATTATCTATATGCCACCTGAAAACTATGAACCTGGACCAAAATCAAGGG
  CCAGTATCAAGCACGATATATATAGCTATGCAGTTATCACATGGGNAAGTGTTATCCAGA
  AAACAGCCCTTTTGAAGATGTCACCAATCCTTTTGCAGATATGTATAGGGTGTCACAAGG
  ACATCGACCTGGTATAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003821 unedited
  GACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTGAAAATAAAAATCCTTTTATGAAACACATTTTTTTTTGAA
  AAACAAGCACTTACATGCTTTTATTTTGAAAGAAATTTAAAAATGGGGATCTAAAAACCA
  CAAGTTTTTCCGGGTAAGGGTGAAAACCCATTTGGTTGGTATCTTTCAATTTTTGGACTA
  TAACTTTGGCAAAATCTTTTCCTTGGATGGCAAAAAGGGCTAAAAATTGGCTGACTTTTG
  AGGGCCTTGTAGGCTTGGGACTAACAAAGTCATAATCCTCTTTTATGAACAAAGCCCTGG
  ACAAAAAGGCATTTAACGACTGGTTAAAGGAGGCTTCTGGCATTTGGGTCACAAAGGCTT
  CCCTTTTGCTCTGGAACCACTGGTGGGCTATACCAAGCTGGAAACGTTTTGAGTTTCCTG
  CAGTTGAAAGTGGATTTATTATTGCTGAAAAACATGGAGTGGTCTTGTGAACACAAAATG
  CACCCCTTTGAAAACCAAAAAAGGTGCTATCCCAACTGTGATTTCCAAGACAGGGATGCC
  GCTTCATAAAATAACAGGCTTGAGCTTTTCTAGATAAAAAATCAATGTCTTGAGGAACTG
  GCAGGAACCTTTAAAGTTCAAGAAACACCCTATTTTTATGGGACTGAGAGGATCCACATG
  ATTCCTCTTGGGGACCATGATTTCACGGTTGTTCACAGATAATTCCTTTTTTTCTTGGCC
  CCTAGGAAATGGCACTTGAACACTTCTGTAACTTGTTTTTTTTAACCGAAAAACACTTCA
  AGAAAAGTATTTTTCAAAGTTTCCAAACTGGTAAAGTCTATTAAAAATTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003821
• Insert Size: 1880 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003821.4, NP_003812.1
• RefSeq Size: 2521 bp
• RefSeq ORF: 1623 bp
• Locus ID: 8767
• UniProt ID: O43353
• Cytogenetics: 8q21.3
• Domains: pkinase, TyrKc, CARD, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Neurotrophin signaling pathway, NOD-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli. [provided by RefSeq, Jul 2008]