USP13 (NM_003940) Human Untagged Clone

CAT#: SC117705

USP13 (untagged)-Human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP13
• Synonyms: IsoT-3; ISOT3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117705 sequence for NM_003940 edited (data generated by NextGen Sequencing)
  ATGCAGCGCCGGGGCGCCCTGTTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCT
  GCAGGAGACATCGGCGAGCTGCTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCC
  GGCGACAGGGTCTACAAGAACGAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGT
  GGACTCTATGTATGCATGAATACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACAT
  TTTCGAAAAACTGGACAGAGTGTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTA
  AGAGGGGCGTCTGGTGGAGCGTTACCAAAAAGGAGGAATTCCAAGATTTTTTTAGATCTA
  GATACTGATGACGATTTAAATAGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTT
  ATATTCCCAGATCACTATGAAATAGCACTACCAAATATTGAGGAGTTACCAGCCCTGGTA
  ACAATTGCTTGTGATGCAGTTCTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGAC
  ACGTGGGAAAATGAATTGCCAGTATCTAAATATGCCAACAACCTCACCCAGCTGGACAAT
  GGAGTCAGGATTCCTCCAAGTGGTTGGAAGTGTGCCAGATGCGACCTGCGAGAAAACCTC
  TGGTTGAATCTGACTGACGGCTCTGTCCTGTGTGGAAAGTGGTTCTTTGACAGCTCTGGG
  GGCAACGGGCATGCGCTGGAGCATTACAGAGACATGGGCTACCCACTAGCCGTGAAACTG
  GGAACCATCACTCCTGACGGGGCAGATGTTTATTCTTTTCAAGAAGAAGAACCTGTTTTG
  GATCCTCATTTGGCCAAGCACTTAGCGCATTTTGGAATTGATATGCTTCATATGCATGGG
  ACAGAGAATGGGCTCCAGGACAATGACATCAAGCTGAGGGTCAGTGAGTGGGAAGTGATC
  CAGGAGTCGGGCACGAAACTGAAGCCAATGTATGGTCCTGGCTACACGGGTCTGAAGAAC
  CTGGGCAACAGCTGCTATCTCAGCTCTGTCATGCAGGCCATCTTCAGCATCCCAGAATTC
  CAGAGAGCGTATGTAGGAAACCTTCCCAGAATATTTGACTACTCGCCTTTAGATCCAACA
  CAAGATTTCAACACACAGATGACTAAGTTAGGACATGGCCTTCTCTCAGGCCAGTATTCA
  AAGCCTCCGGTGAAATCTGAACTCATTGAACAGGTGATGAAGGAGGAGCACAAGCCACAG
  CAGAACGGGATCTCTCCGCGCATGTTTAAGGCCTTTGTAAGCAAGAGCCACCCGGAATTC
  TCCTCTAACAGGCAGCAAGATGCCCAGGAATTCTTCTTGCACCTGGTGAATCTAGTAGAG
  AGGAACCGCATCGGCTCAGAAAACCCAAGCGATGTTTTTCGTTTTTTGGTGGAAGAACGC
  ATTCAGTGCTGTCAGACCCGGAAAGTCCGCTACACGGAGAGGGTGGATTACCTGATGCAG
  TTACCTGTGGCCATGGAGGCGGCAACCAACAAGGATGAACTGATCGCTTATGAACTAACG
  AGAAGGGAAGCAGAAGCAAACAGAAGACCCCTTCCTGAGTTGGTACGTGCCAAGATACCA
  TTTAGTGCCTGCCTTCAGGCCTTCTCTGAACCAGAAAATGTTGATGATTTCTGGAGCAGT
  GCCCTACAAGCAAAGTCTGCGGGTGTGAAAACATCTCGCTTTGCTTCATTCCCTGAATAC
  TTGGTAGTGCAGATAAAGAAGTTCACTTTTGGTCTTGACTGGGTTCCCAAAAAATTTGAT
  GTTTCTATTGATATGCCAGACCTACTTGATATCAACCATCTCCGAGCCAGGGGGTTACAG
  CCAGGAGAGGAAGAACTTCCAGACATCAGCCCCCCCATAGTCATTCCTGATGACTCAAAA
  GATCGCCTGATGAACCAATTGATAGACCCATCAGACATCGATGAGTCATCAGTGATGCAG
  CTGGCCGAGATGGGTTTCCCGCTGGAAGCATGTCGCAAGGCTGTGTACTTCACTGGAAAT
  ATGGGCGCCGAGGTGGCCTTCAACTGGATCATTGTTCACATGGAAGAGCCAGATTTTGCT
  GAGCCGCTGACCATGCCTGGTTATGGAGGGGCAGCTTCTGCTGGAGCCTCTGTTTTTGGT
  GCTTCTGGACTGGATAACCAACCTCCAGAGGAAATCGTAGCTATCATCACCTCCATGGGA
  TTTCAGCGAAATCAGGCTATTCAGGCACTACGAGCAACGAATAATAACCTGGAAAGAGCA
  CTGGATTGGATCTTTAGCCACCCTGAGTTTGAAGAAGACAGTGATTTTGTGATTGAGATG
  GAGAATAATGCCAATGCAAACATTATTTCTGAGGCCAAGCCCGAAGGACCTAGAGTCAAG
  GATGGATCTGGAACATATGAGCTATTTGCATTCATCAGTCACATGGGAACATCCACAATG
  AGTGGTCATTACATTTGCCATATCAAAAAGGAAGGAAGATGGGTGATTTACAATGACCAC
  AAAGTTTGTGCCTCAGAAAGGCCCCCTAAAGACCTGGGCTACATGTACTTTTACCGCAGG
  ATACCAAGCTAA
  
  Clone variation with respect to NM_003940.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003940 unedited
  TATTATGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGCAGCTGGGCC
  GGAGACTCGCCATTGGATTAAAAAAGCGTCGCCAGTCCGCCCCAAGCCCGCGGTGCCCGC
  TCCCGCCCCGCAGCCCGCTCTCCCCGCCCGCCCCGGCTCGGCCGGCTGCCGTTGCCCGCG
  CAGCCCGCCCGTCAGCCCGCTCGCTGGCGCCGCCGCCGCCGGCAGACCCCGCGCTCCGGC
  TCCGGCTCGGCTCGCTCGGCTCCGGTGCGCGCCGAGGCCATGCAGCGCCGGGGCGCCCTG
  TTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCTGCAGGAGACATCGGCGAGCTG
  CTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCCGGCGACAGGGTCTACAAGAAC
  GAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGTGGACTCTATGTATGCATGAAT
  ACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACATTTTCGAAAAACTGGACAGAGA
  CTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTAAGAGGGGCGTCTGGTGGAGCG
  TTACCAAAAAGGAGGAATTCCAAGAATTTTTTAGATCTAGATACTGATGACGATTTAAAT
  AGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTTATATTCCCAGATCACTATGAA
  ATAGCACTACCAAATATTGAGGAGGTACCAGCCCTGGTAACAATTGCTTGTGATGCAGTT
  CTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGACACGTGGGAAAAATGAATTGCC
  AGTATCTAAATATGCCAACAACCTCACCCACCTGGACAATGGAGTCAGGATTCTCTCCAG
  TGGGTTGGAAGTGTGCCACATGCGACCTGCGAGAAAACTTCTGGTGAATCTGACTGAACG
  CTCTGTNCTGTGTGGAAATTGTTTCTTGACACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003940 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCAAATTAAAAAGG
  CGTATGGACTTCTTTTCGCCAATTTTTATATTTGAGGTTTAACTTGGTATACTGCGGTAA
  AAGTACATGTAGCCCAGGTCTTTAGGGGGCCTTTCTGAGGCACAAACTTTGTGGACATTG
  AAAATTACCCATCTAACTTACTTTTTGATATGGCAACAGAAATGACCACTCATTGTGGAT
  GTTTCCATGTGACTGATGAATGCAAATAGCTCATATGCTCCAAATACATACTTGACTCTA
  GGACCTTCAGTCTTGGACTCACAAATAATGATATGCTCTGCAGTTATTGTGCATCTAAAT
  GACAAAATCACTGTCTTCTTAAAACTAAGGGATGGCTAAATATTCAACACAAGGCTCACT
  ATACGCTATTATAACATGCTTTAAATGCTTGCAACACACCGACTACCTTAAAATTCCATG
  GAGGTGATGATTCCTACAATATATATTGACGGCCTGCCTTATCACCCTCCAACTCCTTAA
  AACAGACGCTTCCACTTATAACCTGTCTCCTTCATAACCACACCTGGTAACACACTGAAA
  CACAACACGGTATTTTCCTGTTAACTATGATCCCATTATATTGCTTCTTTCTTATTCATA
  TTCTCCATCTAAATACCCATCCTTACTTACTGTCCCCTCATGATTAACTTTATTTCCTCC
  CATCTACTACCGTCAACTTTAAACTATATCACGGTTTTCTCATTCGCTCCTACCGCCACC
  TTTATACATTACGCAATCACTCTCGCGGATCTTAAATTGCAAAACAAACTCATCCCCCCA
  CACCACACCCACAAAACGTTCCACCTCACCGCTTCGCCTCCCTACCAACCCAAACATTGT
  TACCCCACAATCCTCCACCTACTTCCTATCGCTCCCCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003940
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003940.1, NP_003931.1
• RefSeq Size: 2738 bp
• RefSeq ORF: 2592 bp
• Locus ID: 8975
• UniProt ID: Q92995
• Cytogenetics: 3q26.33
• Domains: UBA, UCH, zf-UBP
• Protein Families: Druggable Genome, Protease
• Gene Summary: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.[UniProtKB/Swiss-Prot Function]