RAB7L1 (RAB29) (NM_003929) Human Untagged Clone

CAT#: SC117696

RAB7L1 (untagged)-Human RAB7, member RAS oncogene family-like 1 (RAB7L1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB7L1
• Synonyms: RAB7L; RAB7L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117696 sequence for NM_003929 edited (data generated by NextGen Sequencing)
  ATGGGCAGCCGCGACCACCTGTTCAAAGTGCTGGTGGTGGGGGACGCCGCAGTGGGCAAG
  ACGTCGCTGGTGCAGCGATATTCCCAGGACAGCTTCAGCAAACACTACAAGTCCACGGTG
  GGAGTGGATTTTGCTCTGAAGGTTCTCCAGTGGTCTGACTACGAGATAGTGCGGCTTCAG
  CTGTGGGATATTGCAGGGCAGGAGCGCTTCACCTCTATGACACGATTGTATTATCGGGAT
  GCCTCTGCCTGTGTTATTATGTTTGACGTTACCAATGCCACTACCTTCAGCAACAGCCAG
  AGGTGGAAACAGGACCTAGACAGCAAGCTCACACTACCCAATGGAGAGCCGGTGCCCTGC
  CTGCTCTTGGCCAACAAGTGTGATCTGTCCCCTTGGGCAGTGAGCCGGGACCAGATTGAC
  CGGTTCAGTAAAGAGAACGGTTTCACAGGTTGGACAGAAACATCAGTCAAGGAGAACAAA
  AATATTAATGAGGCTATGAGAGTCCTCATTGAAAAGATGATGAGAAATTCCACAGAAGAT
  ATCATGTCTTTGTCCACCCAAGGGGACTACATCAATCTACAAACCAAGTCCTCCAGCTGG
  TCCTGCTGCTAG
  
  Clone variation with respect to NM_003929.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003929 unedited
  NGGGGGTCGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACCAGNAG
  GGCGTCTAGGGAATCGAGGTGCCGGCTGCTCCTTCCTCACAATTTGCCCAGACAGGCGGT
  GGGTGGGAATGCCTCACTTCAGTTTGAAGAGGGTCCGGATCCAAAGGGGTTAAAACGAGC
  GACCCCCGATCCCCGACCACACTTCCCGCCTCCCTAAAACGCACACCCCGCTAGCCATGG
  GCAGCCGCGACCACCTGTTCAAAGTGCTGGTGGTGGGGGACGCCGCAGTGGGCAAGACGT
  CGCTGGTGCAGCGATATTCCCAGGACAGCTTCAGCAAACACTACAAGTCCACGGTGGGAG
  TGGATTTTGCTCTGAAGGTTCTCCAGTGGTCTGACTACGAGATAGTGCGGCTTCAGCTGT
  GGGATATTGCAGGGCAGGAGCGCTTCACCTCTATGACACGATTGTATTATCGGGATGCCT
  CTGCCTGTGTTATTATGTTTGACGTTACCAATGCCACTACCTTCAGCAACAGCCAGAGGT
  GGAAACAGGACCTAGACAGCAAGCTCACACTACCCAATGGAGAGCCGGTGCCCTGCCTGC
  TCTTGGCCAACAAGTGTGATCTGTCCCCTTGNGCAGTGAGCCGGGACCAGATTGACCGGT
  TCAGTAAAGAGAACGGTTTCACAGGTTGGACAGAAACATCAGTCAAGGAGAACAAAAATA
  TTAATGAGGCTATGAGAGTCCTCATTGAAAAGATGATGAGAAAATCCACAGAAGATATCA
  TGTCTTTGTCCACCCAGGNGACTACATCAATCTACAAACCAAGTCCTCCAGCTGGGTCTG
  CTGCTAGNAAGNGTTTGGCTTATTTTCATCCCAGTCTGGGGAGGCCTTTAAGTCTCTTCC
  TTTTGGTGCCCACTGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003929 unedited
  NNNNNNTTTACTCTGNNACCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTT
  TTGAGACGGAGTCCTTCTCTGTCACCCAGGCTGAAGTGCGGTGGCATGATCTCGGTTCAC
  TGCAACCTCTGCCTCCCAGTCTCAAGCGATTCTCCCACCTCAGTCTCCAGAGTAGCTGGG
  ATTACAGACACGTGCCATCACGCCTGGCTAATTTTTGTACTTTTAGTAGAAACGCGGGTT
  TTGCCATGCTGACCAGGTTGGTCTCGAATTCCTGGCCTCATGTGATCCGCCTGCCTTGGC
  CTCCCCAAGTGCTGGGATTACAGGCATGAGCCACCATGCCCGGCCAAAAAACTTGACTAT
  TCTTGATGCCAAGACAAGAGAAACTTAATTATGACGTTAGAAGTTTCAAAAATACAGTGA
  CTTCTGGGACACTTCTAGTAGCCAAAAATCCACAGATGCCCAGACCCCCTCCTTCTGCAT
  TCCAGAATCAGATGTTTACATTCCAAGACAGAAAGAATCTGAAACAACGGCCTACAAGAA
  ATTTGGGAAATGACATTTGATCTCATTTTCCTCTCAATTGGAAGTCATGATATCAATGAA
  TATCAAATGGAAAAATAAGCTCAGAAGTAGGTGAGAATTATGATCTCTAAAACGCAGGTG
  CTGATTTCTAATCCTTCTCATAAAATTCCGGATCACAAATTGAGGGCTGATGAGAAGATC
  TTACACCGANAGCACTAACTGGCACTAATGTGAGCCAGCAACATGTGAAAGGCTAATCCN
  AGAGATGCAGAAATGCACATGGGTTCCCAGGTGTCTTTCTAAGTGACAATGGGCCTCCTT
  ACTGGACAGTAGTCAGGAGACAATTCAAATGTACTTAATAAAAATGCCAGGTGGGCAACC
  AAAAGGGAGNAGACTTAAAAGACCTCCAGAACTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003929
• Insert Size: 1690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003929.1, NP_003920.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 612 bp
• Locus ID: 8934
• UniProt ID: O14966
• Cytogenetics: 1q32.1
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Gene Summary: Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).