ARHGEF7 (NM_003899) Human Untagged Clone

CAT#: SC117668

ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGEF7
• Synonyms: BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117668 sequence for NM_003899 edited (data generated by NextGen Sequencing)
  ATGACCGACAATAGCAACAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACC
  AATGAGGACGAGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAG
  GGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTG
  CGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACACTGAAGAGCCCT
  CCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAAT
  ATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTA
  CGGCCATTGCAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAAT
  CTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAG
  TTGCCCGAAGCTCAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAA
  ACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACAC
  AGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTGGGATTCTCGTG
  CTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAAA
  GAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATG
  GCTGCCTTCAAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAG
  CTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGC
  AACGTCACTTACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAA
  AGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGT
  GGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAAAGCTTGAGGAC
  AGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGTG
  TCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAG
  GTCACGTCTGTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCC
  TCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCC
  GCCTACCACACGCTGCCCCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGG
  GGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCG
  CCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTAAGAGCCCTAAG
  ACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTTC
  GCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAA
  GCTTACTGCACCAGCGCCAAAACAAGGCAAACACTCAATTCAAGTTCACGCAAAGAATCT
  GCTCCACAAGTTTTGCTTCCAGAAGAAGAGAAAATTATAGTGGAAGAAACTAAAAGTAAT
  GGTCAGACAGTGATAGAAGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAGGATGAA
  GTTCAAGAATTAAGACAGGACAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGA
  GCCCGCAAAGACCTGGAGAAGCTGGTGAGGAAAGTCCTGAAGAACATGAATGATCCTGCC
  TGGGATGAGACCAATCTATAA
  
  Clone variation with respect to NM_003899.3
  9 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003899 unedited
  CGTTANCATTTGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGGCAAACCGT
  TACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCATCTCGGACCCGGA
  GGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCGCCT
  GCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAG
  CAACATCCGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGACGTTTGATGC
  AAATGATTTGTATCAGGGGCAGAATTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAA
  TAAAGTAACAGCAGACATCGGGCTGGGGAGTGACTCCGTGTGTGCCCGGCCCTCGTCTCA
  CCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCTTTGCACACTCGGACTTCAAAACT
  GTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGACAATAGCAACAATCAACTGGTAGT
  AAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAGGAGAC
  GTCATCCATGTCACCCGTGTGGAATAGGAGGCTGTGGNGGAAGGGCACACTCAACGGCCG
  GACCGGCTGGTTCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCT
  CCCATATCATGACACTGAAGAGCCCTCCAAAGGATTTGATACGACTGCCATANNACAAAC
  ATTACAATGTGGTGCTACAGAATATTTTAGAAACAGATNATGAATATTCTAAAGAATTNC
  AGACTGTGCTTTTCACGTACCTACGGCCATTGCAGACANTGGAAAAAAGTTAAGTTCAGC
  ANACATTTCTNATTTAATGGNGAATNCTAGAAGAATTAATGCCCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003899 unedited
  TGCTCTGGACCGGCGGCCGCAATCTAGGGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTG
  ACATAAGAAAAAAATCAGTTTAATAATTACAACAGTGTAGCATCAACATTGTAGTAGTCA
  ACACTAGGACATTGTCTGTGACATTTAAATTAGGAGTTTCTCCACACTTGTCAACAGGCG
  CCCTGGGTGTCCCAGAGAAGTGAAGAAATGATTGGCATTAAGGAGTCCACACAACCAGGG
  CAGGCATTCAAGGTCCCAGACGCCCAGTGCATGTGGACAAGCACAGACGCCACTGAGTGT
  GAAGGAACGAGCTTCTGATGCTACATTCCAACATCGCTGAAAACTGAAAGTGCTTCACAT
  CTACAGGATAAGAAATGGCTTCATCTCCTCATCCATACAAGGTGAGAGCAAAGGAGTAAC
  CCTCGGGCAAATCGTGGCCCCTTTAAACGTTTATATCAAGTTCCCACCCCCTTGGGAGGT
  CACACGGTGAAACAGACACAGTTATATACAACAGGGCAGGTTTTTAAAAAGAGTTGCTCT
  CAGACGCATTTTTCCTGCTCCCTAAAAAGCCGAGGAAGATACTGGATCCACAGAAAGAAA
  AGGCAATGCCGTAACATGAGGCCCTCATGGCCGCACCGTCCAGGGGAAGGGCTGNTAAAA
  ACACAAGTATTCTTGTNGAATACTTCGATCTGAGCATTAAGGCAGGTCTGCAGGAGATCC
  GTCCTGGGGACTCCGACAGCAACGCTACCGGCTTCGAGAGGACAGTTAATGTCGCCCTCC
  CGCAGAGGGGCCGAGAGATCAAACAAGAGTTGTTCCTGAGTTAAAAACTGCTACACAGCA
  AACTCCCATAACTCAAGGTGAATGTTTTCCACTGGCATGCTTTACGCATACCAAAAATAA
  CTTGTAAACTCAGCCTACCAAAAGAAGTGTTGACTCTGCTGGGCCCCCTGATGAATAACC
  GCTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003899
• Insert Size: 5130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003899.2, NP_003890.1
• RefSeq Size: 5032 bp
• RefSeq ORF: 1941 bp
• Locus ID: 8874
• UniProt ID: Q14155
• Cytogenetics: 13q34
• Domains: RhoGEF, SH3, PH
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains a different 3' coding region and 3' UTR, compared to variant 1. It initiates translation at a downstream start codon. Variants 1, 5, 6, 18, and 19 all encode isoform a (also known as p85Cool-1), which has a shorter N-terminus and a longer and distinct C-terminus, compared to isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.