NFkB Inducing Kinase NIK (MAP3K14) (NM_003954) Human Untagged Clone

SKU SC117658

MAP3K14 (untagged)-Human mitogen-activated protein kinase kinase kinase 14 (MAP3K14)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NFkB Inducing Kinase NIK
• Synonyms: FTDCR1B; HS; HSNIK; NIK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003954 edited; CCACGCGTCCGCGCAACACTCGCTTGGTTGGGGAGATCGGCGCTTGGCCGGATGAGCACA; AGCCTGGGAGATGGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCAGCAGTGGG; GCAGCAGAAGGAACTCCCCAAAGCCAAGGAGAAGACGCCGCCACTGGGGAAGAAACAGAG; CTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCTGTGTTCTGCGGAAAGTGGGAGAT; CCTGAATGACGTGATTACCAAGGGCACAGCCAAGGAAGGCTCCGAGGCAGGGCCAGCTGC; CATCTCTATCATCGCCCAGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCCACCTTTTC; AGAACGCATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTTGATCAGAT; CCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGGAAGGGAAA; GCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCAAAGTCCCTGGCTCATGC; AGGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGAGAGCTGCACCATCCC; AGTGCAGGAGGATGAGTCTCCACTCGGCGCCCCATATGTTAGAAACACCCCGCAGTTCAC; CAAGCCTCTGAAGGAACCAGGCCTTGGGCAACTCTGTTTTAAGCAGCTTGGCGAGGGCCT; ACGGCCGGCTCTGCCTCGATCAGAACTCCACAAACTGATCAGCCCCTTGCAATGTCTGAA; CCACGTGTGGAAACTGCACCACCCCCAGGACGGAGGCCCCCTGCCCCTGCCCACGCACCC; CTTCCCCTATAGCAGACTGCCTCATCCCTTCCCATTCCACCCTCTCCAGCCCTGGAAACC; TCACCCTCTGGAGTCCTTCCTGGGCAAACTGGCCTGTGTAGACAGCCAGAAACCCTTGCC; TGACCCACACCTGAGCAAACTGGCCTGTGTAGACAGTCCAAAGCCCCTGCCTGGCCCACA; CCTGGAGCCCAGCTGCCTGTCTCGTGGTGCCCATGAGAAGTTTTCTGTGGAGGAATACCT; AGTGCATGCTCTGCAAGGCAGCGTGAGCTCAGGCCAGGCCCACAGCCTGACCAGCCTGGC; CAAGACCTGGGCAGCAAGGGGCTCCAGATCCCGGGAGCCCAGCCCCAAAACTGAGGACAA; CGAGGGTGTCCTGCTCACTGAGAAACTCAAGCCAGTGGATTATGAGTACCGAGAAGAAGT; CCACTGGGCCACGCACCAGCTCCGCCTGGGCAGAGGCTCCTTCGGAGAGGTGCACAGGAT; GGAGGACAAGCAGACTGGCTTCCAGTGCGCTGTCAAAAAGGTGCGGCTGGAAGTATTTCG; GGCAGAGGAGCTGATGGCATGTGCAGGATTGACCTCACCCAGAATTGTCCCTTTGTATGG; AGCTGTGAGAGAAGGGCCTTGGGTCAACATCTTCATGGAGCTGCTGGAAGGTGGCTCCCT; GGGCCAGCTGGTCAAGGAGCAGGGCTGTCTCCCAGAGGACCGGGCCCTGTACTACCTGGG; CCAGGCCCTGGAGGGTCTGGAATACCTCCACTCACGAAGGATTCTGCATGGGGACGTCAA; AGCTGACAACGTGCTCCTGTCCAGCGATGGGAGCCACGCAGCCCTCTGTGACTTTGGCCA; TGCTGTGTGTCTTCAACCTGATGGCCTGGGAAAGTCCTTGCTCACAGGGGACTACATCCC; TGGCACAGAGACCCACATGGCTCCGGAGGTGGTGCTGGGCAGGAGCTGCGACGCCAAGGT; GGATGTCTGGAGCAGCTGCTGTATGATGCTGCACATGCTCAACGGCTGCCACCCCTGGAC; TCAGTTCTTCCGAGGGCCGCTCTGCCTCAAGATTGCCAGCGAGCCTCCGCCTGTGAGGGA; GATCCCACCCTCCTGCGCCCCTCTCACAGCCCAGGCCATCCAAGAGGGGCTGAGGAAAGA; GCCCATCCACCGCGTGTCTGCAGCGGAGCTGGGAGGGAAGGTGAACCGGGCACTACAGCA; AGTGGGAGGTCTGAAGAGCCCTTGGAGGGGAGAATATAAAGAACCAAGACATCCACCGCC; AAATCAAGCCAATTACCACCAGACCCTCCATGCCCAGCCGAGAGAGCTTTCGCCAAGGGC; CCCAGGGCCCCGGCCAGCTGAGGAGACAACAGGCAGAGCCCCTAAGCTCCAGCCTCCTCT; CCCACCAGAGCCCCCAGAGCCAAACAAGTCTCCTCCCTTGACTTTGAGCAAGGAGGAGTC; TGGGATGTGGGAACCCTTACCTCTGTCCTCCCTGGAGCCAGCCCCTGCCAGAAACCCCAG; CTCACCAGAGCGGAAAGCAACCGTCCCGGAGCAGGAACTGCAGCAGCTGGAAATAGAATT; ATTCCTCAACAGCCTGTCCCAGCCATTTTCTCTGGAGGAGCAGGAGCAAATTCTCTCGTG; CCTCAGCATCGACAGCCTCTCCCTGTCGGATGACAGTGAGAAGAACCCATCAAAGGCCTC; TCAAAGCTCGCGGGACACCCTGAGCTCAGGCGTACACTCCTGGAGCAGCCAGGCCGAGGC; TCGAAGCTCCAGCTGGAACATGGTGCTGGCCCGGGGGCGGCCCACCGACACCCCAAGCTA; TTTCAATGGTGTGAAAGTCCAAATACAGTCTCTTAATGGTGAACACCTGCACATCCGGGA; GTTCCACCGGGTCAAAGTGGGAGACATCGCCACTGGCATCAGCAGCCAGATCCCAGCTGC; AGCCTTCAGCTTGGTGACCAAAGACGGGCAGCCTGTTCGCTACGACATGGAGGTGCCAGA; CTCGGGCATCGACCTGCAGTGCACACTGGCCCCTGATGGCAGCTTCGCCTGGAGCTGGAG; GGTCAAGCATGGCCAGCTGGAGAACAGGCCCTAACCCTGCCCTCCACCGCCGGCTCCACA; CTGCCGGAAGCAGCCTTCCTGCTCGGTGCACGATGCTGCCCTGAAACACAGGCTCAGCCG; TTCCCAGGGATCTGCCAGCCCCCGGCTCAGCAGTGGGACCAGGGCCTCGCAGCAGCAAGG; TGGGGGCAAGCAGAATGCCTCCCAGGATTTCACACCTGAGCCCTGCCCCACCCTGCTGAG; AAAACACTCCGCCACGTGAAGAGACAGAGGAGGATGGCAGGAGTTACCTCGGGAAACAAA; CAGGATCTTCTCTGCCCTGCTCCAGTCGAGTTGGCCTGACCCGCTTGGATCAGTGACCAT; TTGCTGGCAGACAGGGGAGAGCAGCTTCCAGCCTGGGTCAGAAGGGGTGGGCGAGCCCCT; CGGCCCCTCACCCTCCAGGCTGCTGTGAGAGTGTCAAGTGTGTAAGGGCCCAAACTCAGG; TTCAGTGCAGAACCAGGTCAGCAGGTATGCCCGCCCGTAGGTTAAGGGGGCCCTCTAAAC; CCCTTGCCTGGCCTCACCTGGCCAGCTCACCCCTTTTGGGTGTAGGGGAAAAGAATGCCT; GACCCTGGGAAGGCTCCCTGGTAGAATACACCACACTTTTCAGGTTGTTGCAACACAGGT; CCTGAGTTGACCTCTGGTTCAGCCAAGGACCAAAGAAGGTGTGTAAGTGAAGTGGTTCTC; AGTCCCCAGACATGTGCCCCTTTGCTGCTGGCTACCACTCTTCCCCAGAGCAGCAGGCCC; CGAGCCCCTTCAGGCCCAGCACTGCCCCAGACTCGCTGGCACTCAGTTCCCTCATCTGTA; AAGGTGAAGGGTGATGCAGGATATGCCTGACAGGAACAGTCTGTGGATGGACATGATCAG; TGCTAAGGAAAGCAGCAGAGAGAGACGCTCCGGCGCCCCAGCCCCACTATCAGTGTCCAG; CGTGCTGGTTCCCCAGAGCACAGCTCAGCATCACACTGACACTCACCCTGCCCTGCCCCT; GGCCAGAGGGTACTGCCGACGGCACTTTGCACTCTGATGACCTCAAAGCACTTTCATGGC; TGCCCTCTGGCAGGGCAGGGTAGGGCAGTGACACTGTAGGAGCATAGCAAGCCAGGAGAT; GGGGTGAAGGGACACAGTCTTGAGCTGTCCACATGCATGTGACTCCTCAAACCTCTTCCA; GATTTCTCTAAGAATAGCACCCCCTTCCCCATTGCCCCAGCTTAGCCTCTTCTCCCAGGG; GAGCTACTCAGGACTCACGTAGCATTAAATCAGCTGTGAATCGTCAGGGGGTGTCTGCTA; GCCTCAACCTCCTGGGGCAGGGGACGCCGAGACTCCGTGGGAGAAGCTCATTCCCACATC; TTGCCAAGACAGCCTTTGTCCAGCTGTCCACATTGAGTCAGACTGCTCCCGGGGAGAGAG; CCCCGGCCCCCAGCACATAAAGAACTGCAGCCTTGGTACTGCAGAGTCTGGGTTGTAGAG; AACTCTTTGTAAGCAATAAAGTTTGGGGTGATGACAAATGTTAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003954 unedited; CCTTCGAGATTTCGTAAACGTACTCACTATAGGGNCGTGGCGCGCAATTCCCGGGGATAT; CGTTCGACCCACGCGTCCGCGCAACACTCGCTTGGTTGGGGAGATCGGCGCTTGGCCGGA; TGAGCACAAGCCTGGGAGATGGCAGTGATGGAAATGGCCTGCCCAGGTGCCCCTGGCTCA; GCAGTGGGGCAGCAGAAGGAACTCCCCAAAGCCAAGGAGAAGACGCCGCCACTGGGGAAG; AAACAGAGCTCCGTCTACAAGCTTGAGGCCGTGGAGAAGAGCCCTGTGTTCTGCGGAAAG; TGGGAGATCCTGAATGACGTGATTACCAAGGGCACAGCCAAGGAAGGCTCCGAGGCAGGG; CCAGCTGCCATCTCTATCATCGCCCAGGCTGAGTGTGAGAATAGCCAAGAGTTCAGCCCC; ACCTTTTCAGAACGCATTTTCATCGCTGGGTCCAAACAGTACAGCCAGTCCGAGAGTCTT; GATCAGATCCCCAACAATGTGGCCCATGCTACAGAGGGCAAAATGGCCCGTGTGTGTTGG; AAGGGAAAGCGTCGCAGCAAAGCCCGGAAGAAACGGAAGAAGAAGAGCTCANAGTCCCTG; GCTCATGCANGAGTGGCCTTGGCCAAACCCCTCCCCAGGACCCCTGAGCAGGAGAGCTGC; ACCATCCCAGTGCANGAGGATGAGTCTCCACTCGGCGCCCCATATGTTAGAAACACCCCG; CAGTTCACCAAGCCTCTGAAAGAACCANGCCTNGAGCAACTCTGTTTTAAGCAGCTTGGC; GAGGGCCTACGGCCGGCTCTGCCTCGATCAGGACTCCACAACTGATCAGCCCCTTGGCAT; GTCTGGACCACGTGTGGNAACTGCACCACCCCAGGACGGAGCCCCCTTGCCCTGCCACGC; CCCCTTTCCCTTAAGNAAACTGCTCATACCTTCCATGCACCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003954 unedited; TGNGGGGGGTTCTTTGNNACCGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TAACAATTGTCATCACCCCAAACTTTATTGCTTACAAAAAGTTCTCTACAACCCAAACTC; TGCAGTACCAAGGCTGCAGTTCTTTATGTGCTGGGGGCCGGGGCTCTCTCCCCGGGAGCA; GTCTGACTCAATGTGGACAGCTGGACAAAGGCTGTCTTGGCAAGATGTGGGAATGAGCTT; CTCCCACGGAGTCTCGGCGTCCCCTGCCCCAGGAGGTTGAGGCTAGCAAACACCCCCTGA; CGATTCACAGCTGATTTAATGCTACGTGAGTCCTGAGTAGCTCCCCTGGGAGAAGAGGCT; AAGCTGGGGCAATGGGGAAGGGGGTGCTATTCTTAGAGAAATCTGGAAGAGGTTTGAGGA; GTCACATGCATGTGGACAGCTCAAGACTGTGTCCCTTCACCCCATCTCCTGGCTTGCTAT; GCTCCTACAGTGTCACTGCCCTACCCTGCCCTGCCAGAGGGCAGCCATGAAAGTGCTTTG; AGGTCATCAGAGTGCAAAGTGCCGTCGGCAGTACCCTCTGGCCAGGGGCAGGGCAGGGTG; AGTGTCAGTGTGATGCTGAGCTGTGCTCTGGGGAACCAGCACGCTGGACACTGATAGTGG; GGCTGGGGCGCCGGAACGTCTCTCTCTGCTGCTTTCCTTAGCACTGATCATGTCCATCCA; CAGACTGTTCCTGTCAAGCATATCCTGCATCACCCTTCACCTTTACAGATGAGGGAACTG; AGTGCCAGCGAGTCTGGGGCAGTGCTGGGCCTGAAGGGGCTCTGGGCCTGCTGCTCTGGG; GAAGAGTGGTAGCCAGCANNCAAGGGGCACATGTCTGG;
• Restriction Sites: Please inquire
• ACCN: NM_003954
• Insert Size: 4600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003954.1, NP_003945.1
• RefSeq Size: 4596 bp
• RefSeq ORF: 2844 bp
• Locus ID: 9020
• UniProt ID: Q99558
• Cytogenetics: 17q21.31
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, T cell receptor signaling pathway
• Summary: This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]