p27 KIP 1 (CDKN1B) (NM_004064) Human Untagged Clone

SKU SC117607

CDKN1B (untagged)-Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: p27 KIP 1
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004064 edited; GAATTCGCCATATTGGGCCACTAAAAAAAGGGGGCTCGTCTTTTCGGGGTGTTTTTCTCCCCCTCCCCTG; TCCCCGCTTGCTCACGGCTCTGCGACTCCGACGCCGGCAAGGTTTGGAGAGCGGCTGGGTTCGCGGGACC; CGCGGGCTTGCACCCGCCCAGACTCGGACGGGCTTTGCCACCCTCTCCGCTTGCCTGGTCCCCTCTCCTC; TCCGCCCTCCCGCTCGCCAGTCCATTTGATCAGCGGAGACTCGGCGGCCGGGCCGGGGCTTCCCCGCAGC; CCCTGCGCGCTCCTAGAGCTCGGGCCGTGGCTCGTCGGGGTCTGTGTCTTTTGGCTCCGAGGGCAGTCGC; TGGGCTTCCGAGAGGGGTTCGGGCTGCGTAGGGGCGCTTTGTTTTGTTCGGTTTTGTTTTTTTGAGAGTG; CGAGAGAGGCGGTCGTGCAGACCCGGGAGAAAGATGTCAAACGTGCGAGTGTCTAACGGGAGCCCTAGCC; TGGAGCGGATGGACGCCAGGCAGGCGGAGCACCCCAAGCCCTCGGCCTGCAGGAACCTCTTCGGCCCGGT; GGACCACGAAGAGTTAACCCGGGACTTGGAGAAGCACTGCAGAGACATGGAAGAGGCGAGCCAGCGCAAG; TGGAATTTCGATTTTCAGAATCACAAACCCCTAGAGGGCAAGTACGAGTGGCAAGAGGTGGAGAAGGGCA; GCTTGCCCGAGTTCTACTACAGACCCCCGCGGCCCCCCAAAGGTGCCTGCAAGGTGCCGGCGCAGGAGAG; CCAGGATGTCAGCGGGAGCCGCCCGGCGGCGCCTTTAATTGGGGCTCCGGCTAACTCTGAGGACACGCAT; TTGGTGGACCCAAAGACTGATCCGTCGGACAGCCAGACGGGGTTAGCGGAGCAATGCGCAGGAATAAGGA; AGCGACCTGCAACCGACGATTCTTCTACTCAAAACAAAAGAGCCAACAGAACAGAAGAAAATGTTTCAGA; CGGTTCCCCAAATGCCGGTTCTGTGGAGCAGACGCCCAAGAAGCCTGGCCTCAGAAGACGTCAAACGTAA; ACAGCTCGAATTAAGAATATGTTTCCTTGTTTATCAGATACATCACTGCTTGATGAAGCAAGGAAGATAT; ACATGAAAATTTTAAAAATACATATCGCTGACTTCATGGAATGGACATCCTGTATAAGCACTGAAAAACA; ACAACACAATAACACTAAAATTTTAGGCACTCTTAAATGATCTGCCTCTAAAAGCGTTGGATGTAGCATT; ATGCAATTAGGTTTTTCCTTATTTGCTTCATTGTACTACCTGTGTATATAGTTTTTACCTTTTATGTAGC; ACATAAACTTTGGGGAAGGGAGGGCAGGGTGGGGCTGAGGAACTGACGTGGAGCGGGGTATGAAGAGCTT; GCTTTGATTTACAGCAAGTAGATAAATATTTGACTTGCATGAAGAGAAGCAATTTTGGGGAAGGGTTTGA; ATTGTTTTCTTTAAAGATGTAATGTCCCTTTCAGAGACAGCTGATACTTCATTTAAAAAAATCACAAAAA; TTTGAACACTGGCTAAAGATAATTGCTATTTATTTTTACAAGAAGTTTATTCTCATTTGGGAGATCTGGT; GATCTCCCAAGCTATCTAAAGTTTGTTAGATAGCTGCATGTGGCTTTTTTAAAAAAGCAACAGAAACCTA; TCCTCACTGCCCTCCCCAGTCTCTCTTAAAGTTGGAATTTACCAGTTAATTACTCAGCAGAATGGTGATC; ACTCCAGGTAGTTTGGGGCAAAAATCCGAGGTGCTTGGGAGTTTTGAATGTTAAGAATTGACCATCTGCT; TTTATTAAATTTGTTGACAAAATTTTCTCATTTTCTTTTCACTTCGGGCTGTGTAAACACAGTCAAAATA; ATTCTAAATCCCTCGATATTTTTAAAGATCTGTAAGTAACTTCACATTAAAAAATGAAATATTTTTTAAT; TTAAAGCTTACTCTGTCCATTTATCCACAGGAAAGTGTTATTTTTAAAGGAAGGTTCATGTAGAGAAAAG; CACACTTGTAGGATAAGTGAAATGGATACTACATCTTTAAACAGTATTTCATTGCCTGTGTATGGAAAAA; CCATTTGAAGTGTACCTGTGTACATAACTCTGTAAAAACACTGAAAAATTATACTAACTTATTTATGTTA; AAAGATTTTTTTTAATCTAGACAATATACAAGCCAAAGTGGCATGTTTTGTGCATTTGTAAATGCTGTGT; TGGGTAGAATAGGTTTTCCCCTCTTTTGTTAAATAATATGGCTATGCTTAAAAGGTTGCATACTGAGCCA; AGTATAATTTTTTGTAATGTGTGAAAAAGATGCCAATTATTGTTACACATTAAGTAATCAATAAAGAAAA; CTTCCATAGCTAAAAAAAAAAAAAAAAAAACTCGAC
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004064 unedited; TCTTTCCCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG; CAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCG; AATTCGGCACGAGGGGCCACTAAAAAAAGGGGGCTCGTCTTTTCGGGGTGTTTTTCTCCC; CCTCCCCTGTCCCCGCTTGCTCACGGCTCTGCGACTCCGACGCCGGCAAGGTTTGGAGAG; CGGCTGGGTTCGCGGGACCCGCGGGCTTGCACCCGCCCAGACTCGGACGGGCTTTGCCAC; CCTCTCCGCTTGCCTGGTCCCCTCTCCTCTCCGCCCTCCCGCTCGCCAGTCCATTTGATC; AGCGGAGACTCGGCGGCCGGGCCGGGGCTTCCCCGCAGCCCCTGCGCGCTCCTAGAGCTC; GGGCCGTGGCTCGTCGGGGTCTGTGTCTTTTGGCTCCGAGGGCAGTCGCTGGGCTTCCGA; GAGGGGTTCGGGCTGCGTAGGGGCGCTTTGTTTTGTTCGGTTTTGTTTTTTTGAGAGTGC; GAGAGAGGCGGTCGTGCAGACCCGGGAGAAAGATGTCAAACGTGCGAGTGTCTAACGGGA; GCCCTAGCCTGGAGCGGATGGACGCCAGGCAGGCGGAGCACCCCAAGCCCTCGGCCTGCA; GGAACCTCTTCGGCCCGGTGGACCACGAAGAGTTAACCCGGGACTTGGAGAAGCACTGCA; GAGACATGGAAGAGGCGAGCCAGCGCAAGTGGAATTTCGATTTTCAGAATCACAAACCCC; TAGAGGGCAAGTACGAGTGGCAAGAGGTGGAGAAGGGCANCTTGCCCGATTCTACTACAG; ACCCCCGCGGCCCCCCAAAGTGCCTGCCAGGTGCCCCGCGCAGANAGCANGATGTCAGCG; GGAGCCGCCCCGNCGGCGCCTTTATTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004064 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAGCTATGGAAGTTTTCTTTAT; TGATTACTTAATGTGTAACAATAATTGGCATCTTTTTCACACATTACAAAAAATTATACT; TGGCTCAGTATGCAACCTTTTAAGCATAGCCATATTATTTAACAAAAGAGGGGAAAACCT; ATTCTACCCAACACAGCATTTACAAATGCACAAAACATGCCACTTTGGCTTGTATATTGT; CTAGATTAAAAAAAATCTTTTAACATAAATAAGTTAGTATAATTTTTCAGTGTTTTTACA; GAGTTATGTACACAGGTACACTTCAAATGGTTTTTCCATACACAGGCAATGAAATACTGT; TTAAAGATGTAGTATCCATTTCACTTATCCTACAAGTGTGCTTTTCTCTACATGAACCTT; CCTTTAAAAATAACACTTTCCTGTGGATAAATGGACAGAGTAAGCTTTAAATTAAAAAAT; ATTTCATTNTTTAATGTGAAGTTACTTACAGATCTTTAAAAATATCGAGGGATTTAGAAT; TATTTTGACTGTGTTTACACAGCCCGAAGTGAAAAGAAAATGAGAAAATTTTGTCAACAA; ATTTAATAAAAGCAGATGGTCAATTCTTAACATTCAAACTCCCAAGCACCTCGGATTTTT; GCCCCAACTACCTGGAGTGATCACCATTCTGCTGAGTAANTAACTGGTAAAATCCAACTT; TAGAGAGACTGGNGAGGGCAGTGAGGATAGTTTTCTGNTGCTTTTTTAAAAAGCCACATG; CAGCTATCTACCAAACTTTAGATAGCTTGGGAGATCACCAAATCTTCCAAATGAGAATAA; AACTCCTGGAAAAATAAATAGCAATTATCTTTAGCCACGGGTCCAAATTTTGGGGATTTT; TTTAAATGAAAGATCAGCTGTCTCTGGAAGGGGACTTACATTCTTTAAGAAACCATTCAA; ACCCTTTCCCAAAATGCTTCCCTTCTGCAAGACAAATTTATTTATTGCTGGAAATAAAGC; AGCCTTATACCCGCTCCGAGGAGTTCTTCAGCCCCCCTGCCTCCTTCCAAAGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_004064
• Insert Size: 597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004064.2, NP_004055.1
• RefSeq Size: 2422 bp
• RefSeq ORF: 597 bp
• Locus ID: 1027
• UniProt ID: P46527
• Cytogenetics: 12p13.1
• Domains: CDI
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer
• Summary: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]