Ephrin B2 (EFNB2) (NM_004093) Human Untagged Clone

CAT#: SC117586

EFNB2 (untagged)-Human ephrin-B2 (EFNB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ephrin B2
• Synonyms: EPLG5; Htk-L; HTKL; LERK5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004093, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGTGAGAAGGGACTCCGTGTGGAAGTACTGCTGGGGTGTTTTGATGGTTTTATGCAGAACTGCGA
  TTTCCAAATCGATAGTTTTAGAGCCTATCTATTGGAATTCCTCGAACTCCAAATTTCTACCTGGACAAGG
  ACTGGTACTATACCCACAGATAGGAGACAAATTGGATATTATTTGCCCCAAAGTGGACTCTAAAACTGTT
  GGCCAGTATGAATATTATAAAGTTTATATGGTTGATAAAGACCAAGCAGACAGATGCACTATTAAGAAGG
  AAAATACCCCTCTCCTCAACTGTGCCAAACCAGACCAAGATATCAAATTCACCATCAAGTTTCAAGAATT
  CAGCCCTAACCTCTGGGGTCTAGAATTTCAGAAGAACAAAGATTATTACATTATATCTACATCAAATGGG
  TCTTTGGAGGGCCTGGATAACCAGGAGGGAGGGGTGTGCCAGACAAGAGCCATGAAGATCCTCATGAAAG
  TTGGACAAGATGCAAGTTCTGCTGGATCAACCAGGAATAAAGATCCAACAAGACGTCCAGAACTAGAAGC
  TGGTACAAATGGAAGAAGTTCGACAACAAGTCCCTTTGTAAAACCAAATCCAGGTTCTAGCACAGACGGC
  AACAGCGCCGGACATTCGGGGAACAACATCCTCGGTTCCGAAGTGGCCTTATTTGCAGGGATTGCTTCAG
  GATGCATCATCTTCATCGTCATCATCATCACGCTGGTGGTCCTCTTGCTGAAGTACCGGAGGAGACACAG
  GAAGCACTCGCCGCAGCACACGACCACGCTGTCGCTCAGCACACTGGCCACACCCAAGCGCAGCGGCAAC
  AACAACGGCTCAGAGCCCAGTGACATTATCATCCCGCTAAGGACTGCGGACAGCGTCTTCTGCCCTCACT
  ACGAGAAGGTCAGCGGGGACTACGGGCACCCGGTGTACATCGTCCAGGAGATGCCCCCGCAGAGCCCGGC
  GAACATTTACTACAAGGTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004093 unedited
  NGGTTGCCATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACCAGNAGGATTGG
  GGGTCCCAGCCTGCGTCCCGTCAGTCCCTTCTTGGCCCGGAGTGCGCGGAGCTGGGAGTG
  GCTTCGCCATGGCTGTGAGAAGGGACTCCGTGTGGAAGTACTGCTGGGGTGTTTTGATGG
  TTTTATGCAGAACTGCGATTTCCAAATCGATAGTTTTAGAGCCTATCTATTGGAATTCCT
  CGAACTCCAAATTTCTACCTGGACAAGGACTGGTACTATACCCACAGATAGGAGACAAAT
  TGGATATTATTTGCCCCAAAGTGGACTCTAAAACTGTTGGCCAGTATGAATATTATAAAG
  TTTATATGGTTGATAAAGACCAAGCAGACAGATGCACTATTAAGAAGGAAAATACCCCTC
  TCCTCAACTGTGCCAAACCAGACCAAGATATCAAATTCACCATCAAGTTTCAAGAATTCA
  GCCCTAACCTCTGGGGTCTAGAATTTCAGAAGAACAAAGATTATTACATTATATCTACAT
  CAAATGGGTCTTTGGAGGGCCTGGATACCAGGAGGGAGGGGTGTGCCAGACAAGAGCCAT
  GAAGATCCTCATGANAGTTGGACAAGATGCAAGTTCTGCTGNGATCAACCAGAATAAAGA
  TCCAACAAGACGTCCAGAACTAGAAGCTGGTACANATGGAAGAAGTTCGACACCCAGTCC
  CTTTGTANAACCAAATCCAGGTTCTAGCACAGACCGGCACAGCGCCCGACATTCGGNGAA
  ACACATCCNTCGTTCCGAAGTGGCCTTATTTGCAGGNATTGCTTCAGGATGCATCATCTT
  CATCGNCATCATCATCACGCTGGTGGGTCCTCTTGCTGAAGTCCCGNAGGAGACACAGGA
  AGCACTCGNCGCNACACACGACCACGCTGTCGCTCAGACACTGGCCACACCCAGCGCAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004093 unedited
  NCCCGGCCTTGGGGAATATTTTCATTTTTTAAAAACAAATCATTTGAAACANAGTGGAAT
  GTGACTTCACGGATTTCATTTTCTGGGGCTAACTGCAATGTGAAACTAAAGCAGATTTAA
  AACCTATGACAGGCTATTAAAATAAAACAAAGAAAGAAAAAAATATTTATAACTCAGGCA
  TAATACTGTGTTACTTACAAATTGGACAACGAAATTTTAAATAAATATTCATGGTACATA
  ATTACGGCACAAATATGCAGCAATTTGGCAACCTTTTATACCATTTTTTCCTCATTACAG
  TGCAAAGGGGAATGACACTGCCGTTAAACAAGCTGTAGCTAAATACATTGCAAAATTCAG
  ATTTTATACAAAACATCTTGCTTAGACTTTATAAAAAACCAACATTGCTCTATGTACACA
  ATCTGGGTAAGAAAAGCCATTTCTTTCTTGTTTTCATGTGTATTTTTATTAAAAAGGTGA
  ATAAGGCTACTGTAACACCCCAAATCCATAGACAGTAAAATCTGAACCTATTTACAGCAT
  CTTCACTGAAACATGATGGTGCAAATTCCAAAGTCGGGTGACCAGNGACGATCATACAAG
  CAAGGCATTTACAGTAACTTTCCAAAGCTAAACCAACTTCAAACAAAGGGACAGTTGGTA
  GATTGTCATATTCTGCACCAGACACAAAACAGACGCACAGAACACTTTTCAATTGGTTGC
  AGGGAACTCTTTTATCAATTCATCTGATTTTTCACAGTTTAGCATAGAACCAAAACGTAG
  CCAACTGATGATACATACTTTTGATTTCATTAAAATCAGACTATTTCCCATTTTTTCCCT
  TTNTTGCCCTCTCCCCCAATCCTAAAATTACTTCTGATGGCTTAAAAGAAACTCTTGCTG
  GATTGCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004093
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004093.2, NP_004084.1
• RefSeq Size: 4335 bp
• RefSeq ORF: 1002 bp
• Locus ID: 1948
• UniProt ID: P52799
• Cytogenetics: 13q33.3
• Domains: Ephrin
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]