BAG3 (NM_004281) Human Untagged Clone

CAT#: SC117478

BAG3 (untagged)-Human BCL2-associated athanogene 3 (BAG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAG3
• Synonyms: BAG-3; BIS; CAIR-1; MFM6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004281, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGCCGCCACCCACTCGCCCATGATGCAGGTGGCGTCCGGCAACGGTGACCGCGACCCTTTGCCCC
  CCGGATGGGAGATCAAGATCGACCCGCAGACCGGCTGGCCCTTCTTCGTGGACCACAACAGCCGCACCAC
  TACGTGGAACGACCCGCGCGTGCCCTCTGAGGGCCCCAAGGAGACTCCATCCTCTGCCAATGGCCCTTCC
  CGGGAGGGCTCTAGGCTGCCGCCTGCTAGGGAAGGCCACCCTGTGTACCCCCAGCTCCGACCAGGCTACA
  TTCCCATTCCTGTGCTCCATGAAGGCGCTGAGAACCGGCAGGTGCACCCTTTCCATGTCTATCCCCAGCC
  TGGGATGCAGCGATTCCGAACTGAGGCGGCAGCAGCGGCTCCTCAGAGGTCCCAGTCACCTCTGCGGGGC
  ATGCCAGAAACCACTCAGCCAGATAAACAGTGTGGACAGGTGGCAGCGGCGGCGGCAGCCCAGCCCCCAG
  CCTCCCACGGACCTGAGCGGTCCCAGTCTCCAGCTGCCTCTGACTGCTCATCCTCATCCTCCTCGGCCAG
  CCTGCCTTCCTCCGGCAGGAGCAGCCTGGGCAGTCACCAGCTCCCGCGGGGGTACATCTCCATTCCGGTG
  ATACACGAGCAGAACGTTACCCGGCCAGCAGCCCAGCCCTCCTTCCACCAAGCCCAGAAGACGCACTACC
  CAGCGCAGCAGGGGGAGTACCAGACCCACCAGCCTGTGTACCACAAGATCCAGGGGGATGACTGGGAGCC
  CCGGCCCCTGCGGGCGGCATCCCCGTTCAGGTCATCTGTCCAGGGTGCATCGAGCCGGGAGGGCTCACCA
  GCCAGGAGCAGCACGCCACTCCACTCCCCCTCGCCCATCCGTGTGCACACCGTGGTCGACAGGCCTCAGC
  AGCCCATGACCCATCGAGAAACTGCACCTGTTTCCCAGCCTGAAAACAAACCAGAAAGTAAGCCAGGCCC
  AGTTGGACCAGAACTCCCTCCTGGACACATCCCAATTCAAGTGATCCGCAAAGAGGTGGATTCTAAACCT
  GTTTCCCAGAAGCCCCCACCTCCCTCTGAGAAGGTAGAGGTGAAAGTTCCCCCTGCTCCAGTTCCTTGTC
  CTCCTCCCAGCCCTGGCCCTTCTGCTGTCCCCTCTTCCCCCAAGAGTGTGGCTACAGAAGAGAGGGCAGC
  CCCCAGCACTGCCCCTGCAGAAGCTACACCTCCAAAACCAGGAGAAGCCGAGGCTCCCCCAAAACATCCA
  GGAGTGCTGAAAGTGGAAGCCATCCTGGAGAAGGTACAGGGGCTGGAGCAGGCTGTAGACAACTTTGAAG
  GCAAGAAGACTGACAAAAAGTACCTGATGATCGAAGAGTATTTGACCAAAGAGCTGCTGGCCCTGGATTC
  AGTGGACCCCGAGGGACGAGCCGATGTGCGTCAGGCCAGGAGAGACGGTGTCAGGAAGGTTCAGACCATC
  TTGGAAAAACTTGAACAGAAAGCCATTGATGTCCCAGGTCAAGTCCAGGTCTATGAACTCCAGCCCAGCA
  ACCTTGAAGCAGATCAGCCACTGCAGGCAATCATGGAGATGGGTGCCGTGGCAGCAGACAAGGGCAAGAA
  AAATGCTGGAAATGCAGAAGATCCCCACACAGAAACCCAGCAGCCAGAAGCCACAGCAGCAGCGACTTCA
  AACCCCAGCAGCATGACAGACACCCCTGGTAACCCAGCAGCACCGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004281 unedited
  GTAAACGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCGA
  GGAGGCTATTTCCAGNAACTTCCACCCCTCTCTGGCCACGTCACCCCCGCCTTTAATTCA
  TAAAGGTGCCCGGCGCCGGCTTCCCGGACACGTCGGCGGCGGAGAGGGGCCCACGGCGGC
  GGCCCCGGCCAGAGACTCGGCGCCCGGAGCCAGCGCCCCGCACCCGCGCCCCAGCGGGCA
  GACCCCAACCCAGCATGAGCGCCGCCACCCACTCGCCCATGATGCAGGTGGCGTCCGGCA
  AACGGTGACCGCGACCCTTTGCCCCCCGGATGGGAGATCAAGATCGACCCCGCAGACCGG
  GCTGGCCTTTCTTCGTGGGACCACAACAGCCGCACCACTACGTGGAACGACCCGCGCGTG
  CCCTCTGAGGGCCCCAAAGAGACTCCATCCTCTGCCAATGGCCCTTCCCGGGAGGGCTCT
  AGGCTGCCGCCTGCTAGGGAAAGCCACCCTGTGTACCCCCAGCTCCGACCAGGCTACATT
  CCCATTTCCTGTGCNTCCATGAAGCGCTGANGACCGGCAGGTGCACCCTTTCATGTCTAT
  CCCCACCTGGGATGCAGCGATTCCGAACTGAGGCGGCAGCAGCGGCTCCTCAGAGGTCCC
  AGTCACCTCTGCGGGGCATGCCAGAAACCACTCAGCCAGATAAACAGTGTGGACAGGTGG
  CAGCGGCGGCGGCAGCCCAGCCCCCAGCCTCCCACGGACCTGAGCGGTCCCAGTCTCCAG
  CTGCCTCTGACTGCTCATCCTCATCCTCCTCGGCCAGCCTGCCTTCCTCCGGCAGGAGCA
  GCCTGGGCAGTCACCAGCTCCCGCGGGGGTACATCTCCATTCCGGTGATACACGAGCAGA
  ACGTTACCCCGCCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004281 unedited
  ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTTATATTATTACTTTATTTTC
  TTTTTTTAAAATGTAGCATTAAAGTCATCCAACATACAGATATTCCTATGGCTCCTGGCA
  CATTTTACTCTCTCTAAAGTCAGGTATTTTAATTATGAGATGAAGAAAATCATCTCATTA
  AAATGGCAACATTTCTGATAAATGTTTCATATTTATGTGATGGGTAATTGACTCCCCATC
  TACCCCTCCAGTCCAGAGCTACAAAAGACAGTGCACAACCACAGCTAACAGGTGGTGGGG
  GTGCCCAAGTAGACAGGGCTGCAGAACAACAAGCAACGGGGTTAAACTTCTCAAACAACA
  AGCAACTTCTTTATTTGTACAGAGTAAGAATATAGAAGAAAAGCATCATTTTCCTTTTTA
  GCCCTTTTATTAGTGTTTTGCCTCCACCCAAGTTACTGCATACCAAGCAGCTAATAAAAA
  CCAACTGACTTAAAGTCTCTGAAATGCATGCAACTTATAATTCCCTAAAGCACACATCGG
  TTCCGAGTCTGATTTTTACAGGGCATAAGCTACCGCGCTGCTGGGCTACCAGGGGTGTCT
  GTCATGCCGCTGGGGTTTGAACCCCTGCTGCTGAGGCTTCTGGCCGCTGGGCTTCCGCGC
  GGGGGATCTCTGCATCTCCAGCCCCTTTTTTGCCCTTTGCGGTTCCACGGGACCCCTTTC
  CCTGATTGGCTTCACCCGCCACTCCCGCTCCCAGGGGCTCGGCTAGGATTCTACACCCCG
  ACCTGCCCTGGACTTATTGCCTTCCTCGTAACTTTTTCATAAGGGCGGACCCTCTCCGAC
  CAGGCTTTCTGTCTTCCACCATCACTTTCTCTCCCGGTCCACGATCCACGGACCTCTTCT
  GTGACGATCTCAGCCGCGTAGACTCTTTGTACTACTCTCTTTAAATCTACCATTCCCTAC
  TCCCCTCCCCCCCCGCTGCCCCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004281
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004281.3, NP_004272.2
• RefSeq Size: 2608 bp
• RefSeq ORF: 1728 bp
• Locus ID: 9531
• UniProt ID: O95817
• Cytogenetics: 10q26.11
• Domains: WW, BAG
• Protein Families: Druggable Genome
• Gene Summary: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]