BST2 (NM_004335) Human Untagged Clone

CAT#: SC117439

BST2 (untagged)-Human bone marrow stromal cell antigen 2 (BST2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BST2
• Synonyms: CD317; HM1.24; TETHERIN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117439 sequence for NM_004335 edited (data generated by NextGen Sequencing)
  ATGGCATCTACTTCGTATGACTATTGCAGAGTGCCCATGGAAGACGGGGATAAGCGCTGT
  AAGCTTCTGCTGGGGATAGGAATTCTGGTGCTCCTGATCATCGTGATTCTGGGGGTGCCC
  TTGATTATCTTCACCATCAAGGCCAACAGCGAGGCCTGCCGGGACGGCCTTCGGGCAGTG
  ATGGAGTGTCGCAATGTCACCCATCTCCTGCAACAAGAGCTGACCGAGGCCCAGAAGGGC
  TTTCAGGATGTGGAGGCCCAGGCCGCCACCTGCAACCACACTGTGATGGCCCTAATGGCT
  TCCCTGGATGCAGAGAAGGCCCAAGGACAAAAGAAAGTGGAGGAGCTTGAGGGAGAGATC
  ACTACATTAAACCATAAGCTTCAGGACGCGTCTGCAGAGGTGGAGCGACTGAGAAGAGAA
  AACCAGGTCTTAAGCGTGAGAATCGCGGACAAGAAGTACTACCCCAGCTCCCAGGACTCC
  AGCTCCGCTGCGGCGCCCCAGCTGCTGATTGTGCTGCTGGGCCTCAGCGCTCTGCTGCAG
  TGA
  
  Clone variation with respect to NM_004335.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004335 unedited
  AATCTCTTGGATATTTGTNAATACGAACTTTACTATGAGGGNNCGGCCGCGCAATTCGGC
  ACGAGGTGGATGGCATCTACTTCGTATGACTATTGCAGAGTGCCCATGGAAGACGGGGAT
  AAGCGCTGTAAGCTTCTGCTGGGGATAGGAATTCTGGTGCTCCTGATCATCGTGATTCTG
  GGGGTGCCCTTGATTATCTTCACCATCAAGGCCAACAGCGAGGCCTGCCGGGACGGCCTT
  CGGGCAGTGATGGAGTGTCGCAATGTCACCCATCTCCTGCAACAAGAGCTGACCGAGGCC
  CAGAAGGGCTTTCAGGATGTGGAGGCCCAGGCCGCCACCTGCAACCACACTGTGATGGCC
  CTAATGGCTTCCCTGGATGCAGAGAAGGCCCAAGGACAAAAGAAAGTGGAGGAGCTTGAG
  GGAGAGATCACTACATTAAACCATAAGCTTCAGGACGCGTCTGCAGAGGTGGAGCGACTG
  AGAAGAGAAAACCAGGTCTTAAGCGTGAGAATCGCGGACAAGAAGTACTACCCCAGCTCC
  CAGGACTCCAGCTCCGCTGCGGCGCCCCAGCTGCTGATTGTGCTGCTGGGCCTCAGCGCT
  CTGCTGCAGTGAGATCCCAGGAAGCTGGCACATCTTGGAAGGTCCGTCCTGCTCGGCTTT
  TCGCTTGAACATTCCCTTGATCTCATCAGTTCTGAGCGGGTCATGGGGCAACACGGTTAG
  CGGGGAGAGCACGGGGTAGCCGGAGAAAGGCCTCTGGAGCANGTCTGNGAGGGCCATGGN
  GCAGTCCTGGGTGTGGGGACACAGTNCGGGTTGACCCAGGCTGTCTCCCTCCAGAGCCTC
  CCTNCCGGACATGAGTCCCCCCTCTTGTCTNCCACCCTGAGATTGNGCATGNNGTGCCGT
  GTGNGGGGCATGTGCTGCCCTGTTGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004335 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTCTCCCTCAAAGAAG
  TGTTTATTTTTTGGAGCTCAAAGACCCCAGAAAAAACAACCCCCCCCGCAAAAAAAACCC
  ATAACAACAGGCAGCACATGCCCCCCACACCGCACCCCATGCCCAATCTCAGGGTGGGAG
  ACAAGAGGGGGGACTCATTGTCCGGAGGGAGGCTCTGGAGGGAGACAGCCCTGGGTCAAC
  CCGACTGTGTCCCCACACCCAGGACTGCCCCATGGCCCCTCCAGACCTGCTCCAGAGGCC
  CTTCTCCGGCTACCCCGTGCTCTCCCCGCTAACCGTGTTGCCCCATGACCCGCTCAGAAC
  TGATGAGATCAAGGGAATGTTCAAGCGAAAAGCCGAGCAGGACGGACCTTCCAAGATGTG
  CCAGCTTCCTGGGATCTCACTGCAGCAGAGCGCTGAGGCCCAGCAGCACAATCAGCAGCT
  GGGGCGCCGCAGCGGAGCTGGAGTCCTGGGAGCTGGGGTAGTACTTCTTGTCCGCGATTC
  TCACGCTTAAGACCTGGTTTTCTCTTCTCAGTCGCTCCACCTCTGCAGACCCGGTCTGAA
  GCTTATGGGTTAATGTAGTGATCTCCTCCTCAAGCTCCTTCACTTTCTTTTGTCCTTGGG
  CCTTCTCTGCATCCAGGGAAGCCATTAAGGCCATCCCAGTGGGGTTGCAAGTGGCGGTCT
  GGGCCCTCACATCCCTGAAGCCCTTTTGGGCCTTTGTCATCTCTTGTTGCAAGAGATGGG
  CGACATTGCGACCTTCCTTATTGCCTAAGGGCGTTCTGCAAGGCTTCGTGTTGGCCCTGT
  GGGTAAATAATCACGGCCCCCCCGAATCCCAGATCGGGACCCAGAATTCTTTCCCACAAA
  ACTTCAGGGTTTTCCCCTTTTCCGGGCCCTTGAATGACAACCAAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004335
• Insert Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004335.2, NP_004326.1
• RefSeq Size: 983 bp
• RefSeq ORF: 543 bp
• Locus ID: 684
• UniProt ID: Q10589
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]