PSMA (FOLH1) (NM_004476) Human Untagged Clone

SKU SC117383

FOLH1 (untagged)-Human folate hydrolase (prostate-specific membrane antigen) 1 (FOLH1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PSMA
• Synonyms: FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117383 sequence for NM_004476 edited (data generated by NextGen Sequencing); ATGTGGAATCTCCTTCACGAAACCGACTCGGCTGTGGCCACCGCGCGCCGCCCGCGCTGG; CTGTGCGCTGGGGCGCTGGTGCTGGCGGGTGGCTTCTTTCTCCTCGGCTTCCTCTTCGGG; TGGTTTATAAAATCCTCCAATGAAGCTACTAACATTACTCCAAAGCATAATATGAAAGCA; TTTTTGGATGAATTGAAAGCTGAGAACATCAAGAAGTTCTTATATAATTTTACACAGATA; CCACATTTAGCAGGAACAGAACAAAACTTTCAGCTTGCAAAGCAAATTCAATCCCAGTGG; AAAGAATTTGGCCTGGATTCTGTTGAGCTAGCACATTATGATGTCCTGTTGTCCTACCCA; AATAAGACTCATCCCAACTACATCTCAATAATTAATGAAGATGGAAATGAGATTTTCAAC; ACATCATTATTTGAACCACCTCCTCCAGGATATGAAAATGTTTCGGATATTGTACCACCT; TTCAGTGCTTTCTCTCCTCAAGGAATGCCAGAGGGCGATCTAGTGTATGTTAACTATGCA; CGAACTGAAGACTTCTTTAAATTGGAACGGGACATGAAAATCAATTGCTCTGGGAAAATT; GTAATTGCCAGATATGGGAAAGTTTTCAGAGGAAATAAGGTTAAAAATGCCCAGCTGGCA; GGGGCCAAAGGAGTCATTCTCTACTCCGACCCTGCTGACTACTTTGCTCCTGGGGTGAAG; TCCTATCCAGATGGTTGGAATCTTCCTGGAGGTGGTGTCCAGCGTGGAAATATCCTAAAT; CTGAATGGTGCAGGAGACCCTCTCACACCAGGTTACCCAGCAAATGAATATGCTTATAGG; CGTGGAATTGCAGAGGCTGTTGGTCTTCCAAGTATTCCTGTTCATCCAATTGGATACTAT; GATGCACAGAAGCTCCTAGAAAAAATGGGTGGCTCAGCACCACCAGATAGCAGCTGGAGA; GGAAGTCTCAAAGTGCCCTACAATGTTGGACCTGGCTTTACTGGAAACTTTTCTACACAA; AAAGTCAAGATGCACATCCACTCTACCAATGAAGTGACAAGAATTTACAATGTGATAGGT; ACTCTCAGAGGAGCAGTGGAACCAGACAGATATGTCATTCTGGGAGGTCACCGGGACTCA; TGGGTGTTTGGTGGTATTGACCCTCAGAGTGGAGCAGCTGTTGTTCATGAAATTGTGAGG; AGCTTTGGAACACTGAAAAAGGAAGGGTGGAGACCTAGAAGAACAATTTTGTTTGCAAGC; TGGGATGCAGAAGAATTTGGTCTTCTTGGTTCTACTGAGTGGGCAGAGGAGAATTCAAGA; CTCCTTCAAGAGCGTGGCGTGGCTTATATTAATGCTGACTCATCTATAGAAGGAAACTAC; ACTCTGAGAGTTGATTGTACACCGCTGATGTACAGCTTGGTACACAACCTAACAAAAGAG; CTGAAAAGCCCTGATGAAGGCTTTGAAGGCAAATCTCTTTATGAAAGTTGGACTAAAAAA; AGTCCTTCCCCAGAGTTCAGTGGCATGCCCAGGATAAGCAAATTGGGATCTGGAAATGAT; TTTGAGGTGTTCTTCCAACGACTTGGAATTGCTTCAGGCAGAGCACGGTATACTAAAAAT; TGGGAAACAAACAAATTCAGCGGCTATCCACTGTATCACAGTGTCTATGAAACATATGAG; TTGGTGGAAAAGTTTTATGATCCAATGTTTAAATATCACCTCACTGTGGCCCAGGTTCGA; GGAGGGATGGTGTTTGAGCTAGCCAATTCCATAGTGCTCCCTTTTGATTGTCGAGATTAT; GCTGTAGTTTTAAGAAAGTATGCTGACAAAATCTACAGTATTTCTATGAAACATCCACAG; GAAATGAAGACATACAGTGTATCATTTGATTCACTTTTTTCTGCAGTAAAGAATTTTACA; GAAATTGCTTCCAAGTTCAGTGAGAGACTCCAGGACTTTGACAAAAGCAACCCAATAGTA; TTAAGAATGATGAATGATCAACTCATGTTTCTGGAAAGAGCATTTATTGATCCATTAGGG; TTACCAGACAGGCCTTTTTATAGGCATGTCATCTATGCTCCAAGCAGCCACAACAAGTAT; GCAGGGGAGTCATTCCCAGGAATTTATGATGCTCTGTTTGATATTGAAAGCAAAGTGGAC; CCTTCCAAGGCCTGGGGAGAAGTGAAGAGACAGATTTATGTTGCAGCCTTCACAGTGCAG; GCAGCTGCAGAGACTTTGAGTGAAGTAGCCTAA; ; Clone variation with respect to NM_004476.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004476 unedited; CAAATTTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGACCCCGGT; CTGGAGCGAATTCCAGCCTGCAGGGCTGATAAGCGAGGCATTAGTGAGATTGAGAGAGAC; TTTACCCCGCCGTGGTGGTTGGAGGGCGCGCAGTAGAGCAGCAGCACAGGCGCGGGTCCC; GGGAGGCCGGCTCTGCTCGCGCCGAGATGTGGAATCTCCTTCACGAAACCGACTCGGCTG; TGGCCACCGCGCGCCGCCCGCGCTGGCTGTGCGCTGGGGCGCTGGTGCTGGCGGGTGGCT; TCTTTCTCCTCGGCTTCCTCTTCGGGTGGTTTATAAAATCCTCCAATGAAGCTACTAACA; TTACTCCAAAGCATAATATGAAAGCATTTTTGGATGAATTGAAAGCTGAGAACATCAAGA; AGTTCTTATATAATTTTACACAGATACCACATTTAGCAGGAACAGAACAAAACTTTCAGC; TTGCAAAGCAAATTCAATCCCAGTGGAAAGAATTTGGCCTGGATTCTGTTGAGCTAGCAC; ATTATGATGTCCTGTTGTCCTACCCAAATAAGACTCATCCCAACTACATCTCAATAATTA; ATGAAGATGGAAATGAGATTTTCAACACATCATTATTTGAACCACCTCCTCCAGGATATG; AAAATGTTTCGGATATTGTACCACCTTTTCAGTGCTTTCTCTCCTCAGGGATGCCAGAGG; GCGATCTAGTGTATGTTAACTATGCACGAACTGAAGACTTCCTTTAATTGGGACGGGACA; TGANAATCAANTGCTCTGGGAAAATTGTTATTGCCAGATATGGGAAAGATTTCAGAGGAA; AATAAGGNTANAATGCCCAGCTGGCAGGNGCCAAAGGAGTCATTCTCTACTCCGACCCTG; CTGACTACTTTGCTCCTGGGGTGGAAGTCCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004476 unedited; CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTATAAAATTCAACTTTTTTTCAAATATACCAATTTTAAAATTTATC; AATATACCCATTACGATTTTTTTTGAGGGACATACCACACAAATTCAATACGGATTCTTT; AAAAAATCCTTTTAGGCTACTTCACTCAAAGTCTTTGCAGCTGCCTGCACTGGGAAGGCT; GCAACAAAAATCTGGCTTTTCACTTCTCCCCAGGCCTTGGAAGGGGCCACTTTGCTTTCA; ATATCAAACAGAGCATCATAAATTCCTGGGAATGACTCCCCTGCATACTTGGTGGGGCTG; CTTGGAGCATAAATGACATGCCTATAAAAAGGCCTGTCTGGGAACCCTAATGGATCAAAA; AATGCTCTTTCCAAAAACATGAGTGGACCATTCATCATTCTTAATACTATGGGGGGGCTT; TTGGCAAAGTCCTGGAGTCTCTCACTGAACTTGGAAGCAATTTCTGTAAAATTCTTTACT; GCAGAAAAAAGTGAATCAAATGATACACTGGATGTCTTCATTTCCTGGGGATGTTTCATA; GAAATACTGGAGATTTTGGCAGCATACTTTCTTAAAACTACAGCATAATCTCGACAATCA; AAAGGGAGCACTATGGAATTGGCTAGCTTCAACACCATCCCTCCTCGAACCTGGGCCACA; GGGGAGGGGTATTTAAACATTGGATCTAAAACTTTTCCCCCCACCTAATATGGTTCATAG; ACCCTGGGGAACAGTGTATAACCCCTGAAATTGTTGGTTTCCCAATTTAAATACCGGGCC; TTGGCTGAACAATTCCAGGGGTTGGAAAAACACCCAAAATATTTCCAGACCCAATTGGTT; TACCTGGGAAGCCCCGAACTTTGGGGAAGGATTTTTTAAACCACTTCATAAGAATTGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004476
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004476.1, NP_004467.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 2253 bp
• Locus ID: 2346
• UniProt ID: Q04609
• Cytogenetics: 11p11.12
• Domains: PA, Peptidase_M28, TFR_dimer
• Protein Families: Druggable Genome, Protease, Transmembrane
• Summary: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.