ERAB (HSD17B10) (NM_004493) Human Untagged Clone

CAT#: SC117339

HSD17B10 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 10 (HSD17B10), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERAB
• Synonyms: 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; HSD10MD; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117339 sequence for NM_004493 edited (data generated by NextGen Sequencing)
  ATGGCAGCAGCGTGTCGGAGCGTGAAGGGCCTGGTGGCGGTAATAACCGGAGGAGCCTCG
  GGCCTGGGCCTGGCCACGGCGGAGCGACTTGTGGGGCAGGGAGCCTCTGCTGTGCTTCTG
  GACCTGCCCAACTCGGGTGGGGAGGCCCAAGCCAAGAAGTTAGGAAACAACTGCGTTTTC
  GCCCCAGCCGACGTGACCTCTGAGAAGGATGTGCAAACAGCTCTGGCTCTAGCAAAAGGA
  AAGTTTGGCCGTGTGGATGTAGCTGTCAACTGTGCAGGCATCGCGGTGGCTAGCAAGACG
  TACAACTTAAAGAAGGGCCAGACCCATACCTTGGAAGACTTCCAGCGAGTTCTTGATGTG
  AATCTCATGGGCACCTTCAATGTGATCCGCCTGGTGGCTGGTGAGATGGGCCAGAATGAA
  CCAGACCAGGGAGGCCAACGTGGGGTCATCATCAACACTGCCAGTGTGGCTGCCTTCGAG
  GGTCAGGTTGGACAAGCTGCATACTCTGCTTCCAAGGGGGGAATAGTGGGCATGACACTG
  CCCATTGCTCGGGATCTGGCTCCCATAGGTATCCGGGTGATGACCATTGCCCCAGGTCTG
  TTTGGCACCCCACTGCTGACCAGCCTCCCAGAGAAAGTGTGCAACTTCTTGGCCAGCCAA
  GTGCCCTTCCCTAGCCGACTGGGTGACCCTGCTGAGTATGCTCACCTCGTACAGGCCATC
  ATCGAGAACCCATTCCTCAATGGAGAGGTCATCCGGCTGGATGGGGCCATTCGTATGCAG
  CCTTGA
  
  Clone variation with respect to NM_004493.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004493 unedited
  CTTTTCGGATTTGAAACCGACTTCACTATAGGCGGCCCGCGAATCGGCACGAGGCCGGCG
  ACAAGATGGCAGCAGCGTGTCGGAGCGTGAAGGGCCTGGTGGCGGTAATAACCGGAGGAG
  CCTCGGGCCTGGGCCTGGCCACGGCGGAGCGACTTGTGGGGCAGGGAGCCTCTGCTGTGC
  TTCTGGACCTGCCCAACTCGGGTGGGGAGGCCCAAGCCAAGAAGTTAGGAAACAACTGCG
  TTTTCGCCCCAGCCGACGTGACCTCTGAGAAGGATGTGCAAACAGCTCTGGCTCTAGCAA
  AAGGAAAGTTTGGCCGTGTGGATGTAGCTGTCAACTGTGCAGGCATCGCGGTGGCTAGCA
  AGACGTACAACTTAAAGAAGGGCCAGACCCATACCTTGGAAGACTTCCAGCGAGTTCTTG
  ATGTGAATCTCATGGGCACCTTCAATGTGATCCGCCTGGTGGCTGGTGAGATGGGCCAGA
  ATGAACCAGACCAGGGAGGCCAACGTGGGGTCATCATCAACACTGCCAGTGTGGCTGCCT
  TCGAGGGTCAGGTTGGACAAGCTGCATACTCTGCTTCCAAGGGGGGAATAGTGGGCATGA
  CACTGCCCATTGCTCGGGATCTGGCTCCCATACGTATCCGGGTGATGACCATTGCCCCAG
  GTAGACATATCCCCTCTCTCCATCATACCTGGGATTGGGTGGGATCCATGGACAGTTGAG
  AGGGGAAGGTATCCACCACCTAAGCAGCAGCAGCCCTTTATCTTTGGGCCCCAGAGAAGC
  CCCAGCAGTAGGGCTAACGTACGGAACACCCAGTAATCATGAAACCAAGGTGAAGCTCTG
  AATTTGCTATATGGTTACGCCTGTTGGCACCCCCTGCTGACAACCCCCAGAGAAGAGGCA
  AT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004493 unedited
  AGAGTCGAGTTTTTTTTTTTTTTTTTTGTGAGAAAAAAGACTTTATTAGGCACAGAGGGC
  GACTGGTAGGCAGTTACAAAATGGCTACTGGGCTTCCTCCCAAGCTGGAGAGTACTACCC
  CAGGGAAAGGAAGGGCCGAGGAGCCTGTGTTCTCTCTGCCTTCTCCCTTCAAGGCTGCAT
  ACCAATGGCCCCATCCAGCCGGATGACCTCTCCATTGAGGAATGGGTTCTCGATGATGGC
  CTGTACGAGGTGAGCATACTCACTAGGGTCACCCACTCCGCTAGGGAAGGGCACTTGGCT
  GGCCAACAAGTTGCACACTTTCTCTGGGAGGCTGGTCACCAGTGGGGTGCCAAACATACC
  TAAACAATATACTCACATTCAGAGACTCACCCTTGCTTTCATGACTACCTGGCTCTTCCT
  TACTTTAACCCTACTTATTGGTGCTACTCTGGGGCCCAAACATTAAACGCTGCCGCTGCT
  TACGTCGCGGATACCTTATCCTATCACTGCCCATGGCTCGTACTCCCCCTCCCCCTTCAG
  GTTACAGGCGATCCCCTCACCCCGGCCATGCCCTTCCCCGTTCCCTCTCGACCCCCCTCC
  CCCTTATGGTCATGCCTGCCCCCCCCCCCCTTCCAATCCTTTCCCTTTGCCCCCCTCCCT
  TCCCGTCCCCCCCGTTTTGCCCCCCTTCCTTTCCCCCCCCTGCCCGTCCTTCCCTATCCC
  TCTCCTCCCATCCTCCCACTTCTCCTTCCCGTCCCCATTTCCCTTCTCGTCCTCTTCCGT
  CCGTCCCGTCCCCCTCCTCCCTCCTTCCCTCCCCTCCCTTTTTCTTTCCCCCCTTTTTTG
  TTCCCCCCTTCTCCTCCCCTCTCTTCTCCCCCTCCTTCCCCCCCTTCCCCTCTCCCTCTC
  CCGCTCCCCGCACCCTCTTCCCCTTTTCCCCCCTTTTCCCCCTTTTTCTTCCCCCCCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004493
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004493.1, NP_004484.1
• RefSeq Size: 990 bp
• RefSeq ORF: 963 bp
• Locus ID: 3028
• UniProt ID: Q99714
• Cytogenetics: Xp11.22
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Metabolic pathways, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).