HGS (NM_004712) Human Untagged Clone

CAT#: SC117221

HGS (untagged)-Human hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HGS
• Synonyms: HRS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004712 edited
  GAATTCGGCACGAGGGAACTAAGGGGAGCTGCTGTGTCCCCCGCCTCCTCCTCCCCATTT
  CCGCGCTCCCGGGACCATGTCCGCGCTGGCGGGTGAAGATGTCTGGAGGTGTCCAGGCTG
  TGGGGACCACATTGCTCCAAGCCAGATATGGTACAGGACTGTCAACGAAACCTGGCACGG
  CTCTTGCTTCCGGTGTTCAGAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGA
  TGGGAAGCTCTACTGCCCCAAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTG
  CTCCCTGCTGATGACAGGGCCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTG
  CTTTGCCTGTATGAGCTGCAAGGTGATCATTGAGGATGGGGATGCATATGCACTGGTGCA
  GCATGCCACCCTCTACTGTGGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGA
  GAGACTCTCCACAGAGTCTGTTCAGGAGCAGCTGCCCTACTCTGTCACGCTCATCTCCAT
  GCCGGCCACCACTGAAGGCAGGCGGGGCTTCTCCGTGTCCGTGGAGAGTGCCTGCTCCAA
  CTACGCCACCACTGTGCAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACAATCG
  AAACGCCATCCACCCTGGGGACCGCATCCTGGAGATCAATGGGACCCCCGTCCGCACACT
  TCGAGTGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGAAACAAACAGTCACAAGCAC
  AGGAAGAGGCTGGGGGACTAGAAAGAGGCCCTGCCCTCTAGAAAGCTCAGATCTTGGCTT
  CTGTTACTCATACTCGGGTGGGCTCCTTAGTCAGATGCCTAAAACATTTTGCCTAAAGCT
  CGATGGGTTCTGGAGGACAGTGTGGCTTGTCACAGGCCTAGAGTCTGAGGGAGGGGAGTG
  GGAGTCTCAGCAATCTCTTGGTCTTGGCTTCATGGCAACCACTGCTCACCCTTCAACATG
  CCTGGTTTAGGCAGCAGCTTGGGCTGGGAAGAGGTGGTGGCAGAGTCTCAAAGCTGAGAT
  GCTGAGAGAGATAGCTCCCTGAGCTGGGCCATCTGACTTCTACCTCCCATGTTTGCTCTC
  CCAACTCATTAGCTCCTGGGCAGCATCCTCCTGAGCCACATGTGCAGGTACTGGAAAACC
  TCCATCTTGGCTCCCAGAGCTCTAGGAACTCTTCATCACAACTAGATTTGCCTCTTCTAA
  GTGTCTATGAGCTTGCACCATATTTAATAAATTGGGAATGGGTTTGGGGTATTAATGCAA
  TGTGTGGTGGTTGTATTGGAGCAGGGGGAATTGATAAAGGAGAGTGGTTGCTGTTAATAT
  TATCTTATCTATTGGGTGGTATGTGAAATATTGTACATAGACCTGATGAGTTGTGGGACC
  AGATGTCATCTCTGGTCAGAGTTTACTTGCTATATAGACTGTACTTATGTGTGAAGTTTG
  CAAGCTTGCTTTAGGGCTGAGCCCTGGACTCCCAGCAGCAGCACAGTTCAGCATTGTGTG
  GCTGGTTGTTTCCTGGCTGTCCCCAGCAAGTGTAGGAGTGGTGGGCCTGAACTGGGCCAT
  TGATCAGACTAAATAAATTAAGCAGTTAACATAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004712 unedited
  CCTCGCGGCTCTAGGGCCGGCGCGGTGACGTGCGCGTGCGCGCACTGGAGGGAAAAGGCG
  GAAGCGGAAGTCGGGGGGCGCGCCAGCTCGTAGCAGGGGAGCGCCCGCGGCGTCGGGTTT
  GGGCTGGAGGTCGCCATGGGGCGAGGCAGCGGCACCTTCGAGCGTCTCCTAGACAAGGCG
  ACCAGCCAGCTCCTGTTGGAGACAGATTGGGAGTCCATTTTGCAGATCTGCGACCTGATC
  CGCCAAGGGGACACACAAGCAAAATATGCTGTGAATTCCATCAAGAAGAAAGTCAACGAC
  AAGAACCCACACGTCGCCTTGTATGCCCTGGAGGTCATGGAATCTGTGGTAAAGAACTGT
  GGCCAGACAGTTCATGATGAGGTGGCCAACAAGCAGACCATGGAGGAGCTGAAGGACCTG
  CTGAAGAGACAAGTGGAGGTAAACGTCCGTAACAAGATCCTGTACCTGATCCAGGCCTGG
  GCGCATGCCTTCCGGAACGAGCCCAAGTACAAGGTGGTCCAGGACACCTACCAGATCATG
  AAGGTGGAGGGGCACGTCTTTCCAGAATTCAAAGAGAGCGATGCCATGTTTGCTGCCGAG
  AGAGCCCCAGACTGGGTGGACGCTGAGGAATGCCACCGCTGCAGGGTGCAGTTCGGGGTG
  ATGACCCGTAAGCACCACTGCCGGGCGTGTGGGCANATATTCTGTGGAAAGNGGTCTTNC
  AAGTACTTCACCATNCCCCAAGTTTGGCNTTCCAAAAGGANGTGCCCGTGTGTNAACCCC
  TGCTNCNANCANNTGAACNGGAAACCGAGGGAAAGGCCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004712 unedited
  NTTTTACTCAGNACCGCGNNGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTCTGA
  AAACATTTTATTATCGCTGTACCATTCTGGGGCACTGGAGATGGCAGCTGAGAAGCAGAG
  AAGCTGGAGACCTGAAGCCTCCACCCTTGGCTGTGGCTGCCGAGAGAGGCCCCTGCAGAG
  GGAGCCCCAGGCCACACCCAGACACCACAGCCAGCTCACAGTGCGGCCAGAGGGCACAGG
  CTGCTTGGGGGGCGAGGGTCCCCATTCCTGCCACCTCTCACAGACCATGACCCACAGGGC
  CTGGGGGCTTCCCCTGGGAGAAAGTCCTTCCCTCCCCATGGGCCAGCCCTGGGGAAAGAA
  AGCCGAGACTAACCCACGGACTGCGACAGGCCTGAGGACAAGGGAGGCGGGCTTGGGGTG
  AAGGCCGCCCCTCCCGCGTGAAAAGGGACGCTACCCGACGAGTCGGCCAGCCCGCCGCCA
  TCGCCCCCCCCTCTCACCCCCCCCCCCCCCACCCCCCCCCCCGCACCCACATCCGCCTAC
  CCCCCCCTCCCCCAGTTAACCCCACGCCCGCCTCCCCCCCCCGAACAAAGCCCACACACA
  CCACCCCTCCCGCGCCCCCGCCCCCGGTACGCCTCGCCCCCGCCTCGCCCCCCTACCACC
  CCCGCGCCCCCCCCCCCCCCCCCCCGGCACCCCACCCTGTACCCCCCCCCCACCATGAGA
  CCATAGCCCCCCGCGCCCCCCCCCGACCCCCCGCTCCCCCCCCACCCCCCCCCCCCACGC
  CCCCCCCCCACCCACAACTTGCTCTCCCTCCCCCCCCCCCCCCGTCCTATAACACTCCAC
  CCACACCGCGCCCCCCCCGTCGACCCTTCGCCGCCGCACACCCCCGTAATGACTTAACTC
  CACCCACTCACCCGCCAGTCCCCGACGCCGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004712
• Insert Size: 3220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004712.3, NP_004703.1
• RefSeq Size: 2926 bp
• RefSeq ORF: 2334 bp
• Locus ID: 9146
• UniProt ID: O14964
• Cytogenetics: 17q25.3
• Domains: FYVE, VHS, UIM
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]