ASH2L (NM_004674) Human Untagged Clone

CAT#: SC117193

ASH2L (untagged)-Human ash2 (absent, small, or homeotic)-like (Drosophila) (ASH2L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASH2L
• Synonyms: ASH2; ASH2L1; ASH2L2; Bre2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004674 edited
  GAATTCGGCACGAGGCCAGGGGTGGCCGTGATGGCGGCGGCAGGAGCAGGACCTGGCCAG
  GAAGCGGGTGCCGGGCCTGGCCCAGGAGCGGTCGCAAATGCAACAGGGGCAGAAGAGGGG
  GAGATGAAGCCGGTGGCAGCGGGAGCAGCCGCTCCTCCTGGAGAGGGGATCTCTGCTGCT
  CCGACAGTTGAGCCCAGTTCCGGGGAGGCTGAAGGCGGGGAGGCAAACTTGGTCGATGTA
  AGCGGTGGCTTGGAGACAGAATCATCTAATGGAAAAGATACACTAGAAGGTGCTGGGGAT
  ACATCAGAGGTGATGGATACTCAGGCGGGCTCCGTGGATGAAGAGAATGGCCGACAGTTG
  GGTGAGGTAGAGCTGCAATGTGGGATTTGTACAAAATGGTTCACGGCTGACACATTTGGC
  ATAGATACCTCATCCTGTCTACCTTTCATGACCAACTACAGTTTTCATTGCAACGTCTGC
  CATCACAGTGGGAATACCTATTTCCTCCGGAAGCAAGCAAACTTGAAGGAAATGTGCCTT
  AGTGCTTTGGCCAACCTGACATGGCAGTCCCGAACACAGGATGAACATCCGAAGACAATG
  TTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAGTGCATGACAACC
  AGACAGAGACCTGGGAAAATGACTTGGCCAAATAACATTGTTAAAACAATGAGTAAAGAA
  AGAGATGTATTCTTGGTAAAGGAACACCCAGATCCAGGCAGTAAAGATCCAGAAGAAGAT
  TACCCCAAATTTGGACTTTTGGATCAGGACCTTAGTAACATTGGTCCTGCTTATGACAAC
  CAAAAACAGAGCAGTGCTGTGTCTACTAGTGGGAATTTAAATGGGGGAATTGCAGCAGGA
  AGCAGCGGAAAAGGACGAGGAGCCAAGCGCAAACAGCAGGATGGAGGGACCACAGGGACC
  ACCAAGAAGGCCCGGAGTGACCCTTTGTTTTCTGCTCAGCGCCTTCCCCCTCATGGCTAC
  CCATTGGAACACCCGTTTAACAAAGATGGCTATCGGTATATTCTAGCTGAGCCTGATCCG
  CACGCCCCTGACCCCGAGAAGCTGGAACTTGACTGCTGGGCAGGAAAACCTATTCCTGGA
  GACCTCTACAGAGCCTGCTTGTATGAACGGGTTTTGTTAGCCCTACATGATCGAGCTCCC
  CAGTTAAAGATCTCAGATGACCGGCTGACTGTGGTTGGAGAGAAGGGCTACTCTATGGTG
  AGGGCCTCTCATGGAGTACGGAAGGGTGCCTGGTATTTTGAAATCACTGTGGATGAGATG
  CCACCAGATACCGCTGCCAGACTGGGTTGGTCCCAGCCCCTAGGAAACCTTCAAGCTCCT
  TTAGGTTATGATAAATTTAGCTATTCTTGGCGGAGCAAAAAGGGAACCAAGTTCCACCAG
  TCCATTGGCAAACACTACTCTTCTGGCTATGGACAGGGAGACGTCCTGGGATTTTATATT
  AATCTTCCTGAAGACACAGAGACAGCCAAGTCATTGCCAGACACATACAAAGATAAGGCT
  TTGATAAAATTCAAGAGTTATTTGTATTTTGAGGAAAAAGACTTTGTGGATAAAGCAGAG
  AAGAGCCTGAAGCAGACTCCCCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAA
  GGTGTGGCTTACAAAGATATTTTTGAGGGGGTTTACTTCCCAGCCATCTCACTGTACAAG
  AGCTGCACGGTTTCCATTAACTTTGGACCATGCTTCAAGTATCCTCCGAAGGATCTCACT
  TACCGCCCTATGAGTGACATGGGCTGGGGCGCCGTGGTAGAGCACACCCTGGCTGACGTC
  TTGTATCACGTGGAGACAGAAGTGGATGGGAGGCGCAGTCCCCCATGGGAACCCTGACCA
  GGTCCCTCTTTTCTGTCAAGGACTTTCTGGGAATAATACTGGGGGTTTGTTTTGTTTTGA
  ACTGTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004674 unedited
  NAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCCGAGGCCAGGGTGGCC
  GTGATGGCGGCGGCAGGAGCAGGACCTGGCCAGGAAGCGGGTGCCGGGCCTGGCCCAGGA
  GCGGTCGCAAATGCAACAGGGGCAGAAGAGGGGGAGATGAAGCCGGTGGCAGCGGGAGCA
  GCCGCTCCTCCTGGAGAGGGGATCTCTGCTGCTCCGACAGTTGAGCCCAGTTCCGGGGAG
  GCTGAAGGCGGGGAGGCAAACTTGGTCGATGTAAGCGGTGGCTTGGAGACAGAATCATCT
  AATGGAAAAGATACACTAGAAGGTGCTGGGGATACATCAGAGGTGATGGATACTCAGGCG
  GGCTCCGTGGATGAAGAGAATGGCCGACAGTTGGGTGAGGTAGAGCTGCAATGTGGGATT
  TGTACAAAATGGTTCACGGCTGACACATTTGGCATAGATACCTCATCCTGTCTACCTTTC
  ATGACCAACTACAGTTTTCATTGCAACGTCTGCCATCACAGTGGGAATACCTATTTCCTC
  CGGAAGCAAGCAAACTTGAAGGAAATGTGCCTTAGTGCTTTGGCCAACCTGACATGGCAG
  TCCCGAACACAGGATGAACATCCGAAGACCATGTTCTCCAAAGATAAGGATATTATACCA
  TTTATTGATAAATACTGGGAGTGCATGACAACCAGACAGAGACCTGNGAAAATGACTTGG
  CCAAATAACATTGNTAAAACATGAGNTAAGAAAGAGATGTATTCTTGGTAAAGGAACACC
  CAGATCCCAGCAGTANAGATCCAGAAGAAAGATACCCNCAATTNGACTTTTGGGATCAGA
  CCTTTAGTACATGGNTCCTGCTNATGACACCCAAAACAGAGCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004674 unedited
  CGCGGGCCGCAATCTAGNAGTCGAGTCCNNNNTCTCCCCTTTTCCCCCCTTCCCTTTTTT
  TTTTTTTTTCTTTTCCCCTTTCCCAAACCTTCAAAACAAGTTTTATTTGGGAAGTTTCTC
  ATTTTGTTAAAGCTGCAAGCAAGTTTTTTTCACAATCACCCTCCCAAAAATCTAGTAGTT
  TTACGGATCTCAGGGTCCAAAAGGGTTTTTCAAAATGAACAAACCCAACCCCCCCCCAAA
  AGGAAATAGGGTTAAAATGGGTTCGGAATGTATAGCCATTGTTTTCAGGGGTTTCAAAAA
  CCAGGTTCCCATTTCTTTGGAAAACAGGGGGGATACAGCCAATGCCCCAAGAAGGAAAGG
  TTTTTTAAATAAGTTAGCTCCTCGGACAATATTATCCCCTCCTTTAAAAAATATAGGTGG
  GAAAACACGTCACAGCTGGGGAGGGTTTGGCAGTCCAGAACCCGTTTTCACAGGGGGCTT
  ATGCCATTCCTGGAACCAGCGGTCGCCGGCCTTAGGGCCCACAAAAAAAAACCCCCCTCT
  GGAAGTGGGACAAAATGGCGAAGGTTTCNCCTTTTCCCCAGTCCTCCCAATCCTTTAATT
  TGTAAAAGGAAAGGTTGGGTTTTCACCCTTTTCGGAAACCTTCGGAAACGTCAAAAACAA
  AACAACCCCCCAGTTTCTCCCAAAAAGCCCTTGACAAAAAAAGGCACCGCTAGGGTCTCC
  ATGGGGACTGGGTCTTCTTCCCTTTTGCTCCCCGGGAACCAAAGTCCCCCGGGGGGGTCT
  TTACCGGGGCCCCCCCCTTTGCTTTTTAAGGCGGGAGGCGAACCCTCCGGGGTATCTTTA
  ACCGGGTCCACATCATGGAACCTGCGCCCTCTTTGCCGCGGAAGGGCGGGGAAAAAACCC
  CCCCAAACATTCCTTGACGCCCCCTCGTTGGGCCCCCTTTTTAAATATTTTCCTCTTTGG
  GGGGCGTGGTCCGGGTCTTTCTTTGCTTTACCAANGATTCTTCCCCAAACACAAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004674
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004674.1, NP_004665.1
• RefSeq Size: 2381 bp
• RefSeq ORF: 1887 bp
• Locus ID: 9070
• UniProt ID: Q9UBL3
• Cytogenetics: 8p11.23
• Domains: SPRY
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.