Clathrin heavy chain (CLTC) (NM_004859) Human Untagged Clone

CAT#: SC117104

CLTC (untagged)-Human clathrin, heavy chain (Hc) (CLTC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Clathrin heavy chain
• Synonyms: CHC; CHC17; CLH-17; CLTCL2; Hc; MRD56
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004859 edited
  GAATTCGGCACGAGGGCCCGGGCAGCCACTGCCCCGCAGCCCCAGTGACAGGAGGAGACC
  ATAACCCCCGACAGCGCCATGGCCCAGATTCTGCCAATTCGTTTTCAGGAGCATCTCCAG
  CTCCAGAACCTGGGTATCAACCCAGCAAACATTGGCTTCAGTACCCTGACTATGGAGTCT
  GACAAATTCATCTGCATTAGAGAAAAAGTAGGAGAGCAGGCCCAGGTGGTAATCATTGAT
  ATGAATGACCCAAGTAATCCAATTCGAAGACCAATTTCAGCAGACAGCGCCATCATGAAT
  CCAGCTAGCAAAGTAATTGCACTGAAAGCTGGGAAAACTCTTCAGATTTTTAACATTGAA
  ATGAAAAGTAAAATGAAGGCTCATACCATGACTGATGATGTCACCTTTTGGAAATGGATC
  TCTTTGAATACGGTTGCTCTTGTTACGGATAATGCAGTTTATCACTGGAGTATGGAAGGA
  GAGTCTCAGCCAGTGAAAATGTTTGATCGCCATTCTAGCCTTGCAGGGTGCCAGATTATC
  AATTACCGTACAGATGCAAAACAAAAGTGGTTACTTCTGACTGGTATATCTGCACAGCAA
  AATCGTGTGGTGGGAGCTATGCAGCTATATTCTGTAGATAGGAAAGTGTCTCAGCCCATT
  GAAGGACATGCAGCTAGCTTTGCACAGTTTAAGATGGAAGGAAATGCAGAAGAATCAACG
  TTATTTTGTTTTGCAGTTCGGGGCCAAGCTGGAGGGAAGTTACATATTATTGAAGTTGGC
  ACACCACCTACAGGGAACCAGCCCTTTCCAAAGAAGGCAGTGGATGTCTTCTTTCCTCCA
  GAAGCACAAAATGATTTTCCTGTTGCAATGCAGATCAGTGAAAAGCATGATGTGGTGTTC
  TTGATAACCAAGTATGGTTATATCCACCTCTATGATCTTGAGACTGGTACCTGCATCTAC
  ATGAATAGAATCAGTGGAGAAACAATTTTTGTTACTGCACCTCATGAAGCCACAGCTGGA
  ATAATTGGAGTAAACAGAAAGGGACAAGTTCTGTCAGTGTGTGTGGAAGAAGAAAACATA
  ATTCCTTACATCACCAATGTTCTACAAAATCCTGATTTGGCTCTGAGAATGGCTGTACGT
  AATAACTTAGCCGGTGCTGAAGAACTCTTTGCCCGGAAATTTAATGCTCTTTTTGCCCAG
  GGAAATTACTCGGAGGCAGCAAAGGTGGCTGCTAATGCACCAAAGGGAATTCTTCGTACT
  CCAGACACTATCCGTCGGTTCCAGAGTGTCCCAGCCCAGCCAGGTCAAACTTCTCCTCTA
  CTTCAGTACTTTGGTATCCTTTTGGACCAGGGACAGCTCAACAAATACGAATCCTTAGAG
  CTTTGTAGGCCTGTACTTCAGCAAGGGCGAAAACAGCTTTTGGAGAAATGGTTAAAAGAA
  GATAAGCTGGAATGTTCTGAAGAACTGGGTGATCTTGTGAAATCTGTGGACCCTACATTG
  GCACTTAGTGTGTACCTAAGGGCTAACGTCCCAAATAAAGTCATTCAGTGCTTTGCAGAA
  ACAGGTCAAGTCCAAAAGATTGTTTTATATGCTAAAAAAGTTGGATACACTCCAGATTGG
  ATATTTCTGCTGAGAAATGTAATGCGAATCAGTCCAGATCAGGGACAGCAGTTTGCCCAA
  ATGTTAGTTCAAGATGAAGAGCCTCTTGCTGACATCACACAGATTGTAGATGTCTTTATG
  GAATACAATCTAATTCAGCAGTGTACTGCATTCTTGCTTGATGCTCTGAAGAATAATCGC
  CCATCTGAAGGTCCTTTACAGACGCGGTTACTTGAGATGAACCTTATGCATGCGCCTCAA
  GTTGCAGATGCTATTCTAGGCAATCAGATGTTCACACATTATGACCGGGCTCATATTGCT
  CAACTGTGTGAAAAGGCTGGCCTACTGCAGCGTGCATTAGAACATTTCACTGATTTATAT
  GATATAAAACGTGCAGTGGTTCACACCCATCTTCTTAACCCTGAGTGGTTAGTCAACTAC
  TTTGGTTCCTTATCAGTAGAAGACTCCCTAGAATGTCTCAGAGCCATGCTGTCTGCCAAC
  ATCCGTCAGAATCTGCAGATTTGTGTTCAGGTGGCTTCTAAATATCATGAACAACTGTCA
  ACTCAGTCTCTGATTGAACTTTTTGAATCTTTCAAGAGTTTTGAAGGTCTCTTTTATTTT
  CTGGGATCCATTGTTAACTTTAGCCAGGACCCAGATGTGCACTTTAAATATATTCAGGCA
  GCTTGCAAGACTGGGCAAATCAAAGAAGTAGAAAGAATCTGTAGAGAAAGCAACTGCTAC
  GATCCTGAGCGAGTCAAGAATTTTCTTAAGGAAGCAAAACTAACAGATCAGCTACCACTT
  ATCATTGTGTGTGATCGATTTGACTTTGTCCATGATTTGGTGCTCTATTTATATAGAAAT
  AATCTTCAAAAGTATATAGAGATATATGTACAGAAGGTGAATCCAAGTCGACTTCCTGTA
  GTTATTGGAGGATTACTTGATGTTGACTGTTCTGAAGATGTCATAAAAAACTTGATTCTT
  GTTGTAAGAGGTCAATTCTCTACTGATGAGCTTGTTGCTGAGGTTGAAAAAAGAAACAGA
  TTGAAACTGCTTCTGCCTTGGCTAGAGGCCAGAATTCATGAGGGCTGTGAGGAGCCTGCT
  ACTCACAATGCCTTAGCCAAAATCTACATAGACAGTAATAACAACCCGGAGAGATTTCTT
  CGTGAAAATCCCTACTATGACAGTCGCGTTGTTGGAAAGTATTGTGAGAAGAGAGATCCA
  CATCTGGCCTGTGTTGCTTATGAACGTGGCCAATGTGATCTGGAACTTATTAATGTTTGC
  AATGAGAATTCCCTCTTCAAAAGTCTTTCTCGCTACCTGGTACGTCGAAAGGATCCAGAA
  TTGTGGGGCAGCGTGCTGCTGGAAAGCAATCCTTACAGGAGACCCCTAATTGACCAGGTT
  GTACAAACAGCTTTGTCTGAGACTCAGGACCCTGAAGAAGTGTCAGTAACTGTAAAGGCT
  TTCATGACTGCAGACCTTCCTAATGAACTCATTGAACTGCTGGAGAAAATTGTCCTTGAT
  AACTCTGTATTCAGTGAACACAGGAATCTGCAAAACCTCCTTATCCTCACTGCAATTAAG
  GCTGACCGTACACGTGTTATGGAGTATATTAACCGCCTGGATAATTATGATGCCCCAGAT
  ATTGCCAATATCGCCATCAGCAATGAGCTGTTTGAAGAAGCATTTGCCATTTTCCGGAAA
  TTTGATGTCAATACTTCAGCAGTTCAGGTCTTAATTGAGCATATTGGAAACTTGGATCGG
  GCATATGAGTTTGCTGAACGTTGCAATGAACCTGCGGTCTGGAGTCAACTTGCAAAAGCC
  CAGTTGCAGAAAGGAATGGTGAAAGAAGCCATTGATTCTTATATCAAAGCAGATGATCCT
  TCCTCCTACATGGAAGTTGTTCAGGCTGCCAATACTAGTGGAAACTGGGAAGAACTGGTG
  AAGTACTTGCAGATGGCCCGTAAGAAGGCTCGAGAGTCCTATGTGGAGACAGAACTGATA
  TTCGCACTGGCTAAAACAAACCGCCTTGCAGAGTTAGAAGAATTTATCAATGGACCAAAT
  AATGCTCATATCCAACAAGTTGGTGACCGTTGTTATGATGAAAAAATGTATGATGCTGCT
  AAGTTGTTGTACAATAATGTTTCCAATTTTGGACGTTTGGCATCTACCCTGGTTCACCTG
  GGTGAATATCAGGCAGCTGTTGATGGGGCTAGGAAAGCTAACAGTACTCGAACATGGAAA
  GAGGTCTGCTTCGCCTGTGTAGATGGGAAAGAATTCCGTCTTGCTCAGATGTGTGGACTT
  CATATTGTTGTACATGCAGATGAATTAGAAGAACTTATCAACTACTATCAGGATCGTGGC
  TATTTTGAAGAGCTGATCACCATGTTGGAAGCAGCACTGGGACTTGAGCGAGCTCACATG
  GGAATGTTTACTGAATTAGCTATTCTATACTCTAAATTTAAGCCTCAGAAAATGAGGGAG
  CACCTGGAGCTGTTCTGGTCTAGAGTGAATATTCCCAAGGTGCTAAGAGCTGCAGAACAA
  GCTCATCTTTGGGCAGAACTGGTGTTTTTGTATGACAAGTATGAAGAATATGATAATGCC
  ATAATTACCATGATGAATCATCCAACTGATGCCTGGAAAGAAGGGCAATTCAAAGATATC
  ATTACCAAGGTTGCCAATGTGGAACTATACTACAGAGCAATACAGTTCTACTTAGAATTC
  AAGCCTCTGTTGTTAAATGATTTGCTGATGGTGCTGTCTCCACGGTTGGATCACACTCGT
  GCAGTCAATTATTTCAGCAAGGTTAAACAGCTACCACTGGTGAAACCGTATTTGCGTTCA
  GTTCAGAACCATAACAACAAATCTGTGAATGAATCATTGAACAATCTTTTTATTACAGAA
  GAAGATTATCAGGCTCTGCGAACATCAATAGATGCTTATGACAACTTTGACAATATCTCG
  CTTGCTCAGCGTTTGGAAAAACATGAACTCATTGAGTTCAGGAGAATTGCTGCTTATCTC
  TTCAAAGGCAACAATCGCTGGAAACAGAGTGTAGAGCTGTGCAAGAAAGACAGCCTTTAC
  AAGGATGCAATGCAGTATGCTTCTGAATCTAAAGATACTGAATTGGCTGAAGAACTCCTG
  CAGTGGTTTTTGCAGGAAGAAAAAAGAGAGTGCTTTGGAGCTTGTCTGTTTACCTGTTAC
  GATCTTTTAAGGCCAGATGTCGTCCTAGAAACTGCATGGAGGCACAATATCATGGATTTT
  GCCATGCCCTATTTCATCCAGGTCATGAAGGAGTACTTGACAAAGGTGGATAAATTAGAT
  GCTTCAGAATCACTGAGAAAAGAAGAAGAACAAGCTACAGAGACACAACCCATTGTTTAT
  GGTCAGCCCCAGTTGATGCTGACAGCAGGACCCAGTGTTGCCGTCCCTCCCCAGGCACCT
  TTTGGTTATGGTTATACCGCACCACCGTATGGACAGCCACAGCCTGGCTTTGGGTACAGC
  ATGTGAGATGAAGCGCTGATCCTGTAGTCACCTATTTTCGTACTGAAACATCGTCTTTAC
  CCACTTCTCAGTTTATAATGGGGGAAAACAGGCAACGTGTTCTTGTAACCTTTATTTCAT
  GAAGGACTTCTTTTTGTTTCTAACTATAAACTTGGATCACCTATGTTAAAACCTTATTTC
  ACATTCCACATCATTTTAGAATTTATTTTCGAAGGGGAATAGTTTCAATGTTTTATTCAC
  TTGGGCTTTTTTTCTTCCCCCTCTTTCTTTAAAGAACTGCTCAATATTCAATCTGTTGTG
  AAGAACCTGATTTGCACTCTGTAGTGTTTAAAGAAACAAAGAAACTCTAATATTGAATCT
  CTTAAATTTAGTGTATGTAAACAGCTTACAAATACGTATTGTCTAAATGCATTTAAATCT
  GTTTTATTCAAAGAAAAGCTAAAGCAAAAACACTGGCATATGACCATGCAAGACTGTCAG
  TGCCAACAAAGACAACACTAATCAGCACATCGTACACTGGATTGCAGTGCTTCCCAGATT
  ATTGAAAAATGTTACAGACAACTTGCCTGATTTTTAAATGAGCGTAAAAGGCCCTCTAAC
  CTATGCAGGTTTCCCCATTATGCATATAGAAAATGCTAGTATGTTTTGCTCACTTCATAT
  GTAACAGGTGCCCTTATGTTGTGCTGTATCCTGTGCTTTTTCTGTGGGACCATTCCATTC
  AGGAGCAAAGAGCACCATGATTCCAATCTTGTGTGTGTTTACTAACCCTTCCCTGAGGTT
  TGTGTATGTTGGATATTGTGGTGTTTTAGATCACTGAGTGTACAGAAGAGAGAAATTCAA
  ACAAAATATTGCTGTTCTTCAGTTTTGTTTGTGGAATTTGAAATTACTCAAATTTAAAAT
  AAATTACTGGACTGTGGAAATAACATAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004859 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCGGGCAGCCACTGCCCCGCAG
  CCCCAGTGACAGGAGGAGACCATAACCCCCGACAGCGCCATGGCCCAGATTCTGCCAATT
  CGTTTTCAGGAGCATCTCCAGCTCCAGAACCTGGGTATCAACCCAGCAAACATTGGCTTC
  AGTACCCTGACTATGGAGTCTGACAAATTCATCTGCATTAGAGAAAAAGTAGGAGAGCAG
  GCCCAGGTGGTAATCATTGATATGAATGACCCAAGTAATCCAATTCGAAGACCAATTTCA
  GCAGACAGCGCCATCATGAATCCAGCTAGCAAAGTAATTGCACTGAAAGCTGGGAAAACT
  CTTCAGATTNTTAACATTGAAATGAAAAGTAAAATGAAGGCTCATACCATGACTGATGAT
  GTCACCTTTTAGAAATGGATCTCTTTGAATACCGTTGCCTCTGTTACGGATAATGCATTT
  ATTACTGGGAATTGGAAAGGAAGACTTCACCCGGGAAAAGTTGGATCCCCCTTTTTAACC
  TTGGGGGGGGCAAATATTTATTCCCCCCAAAAGGAAAAAAAAAGGGGGGCCTTCTGCGGG
  GGTTTTTCCCACAAAAAAAATGGGGGGGGGGGGACCCCCCCCTTTCTTTTTAAAAGAAAA
  AGAGGAGTGCCCCCCAAAGAAGAAAAACCCCTTTTTTCCCACCCCCGGGGGGGGGGGGGG
  GGCGAAAAACCCTTCTTTTTTTTTTTTTTTTTCGGCGGCCGCGCGGGGGGGGGAGATTTT
  TTTTTTTTTTTCTTCCCCCCCACCCCACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004859 unedited
  GACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTATTT
  CCACAGTCCAGTAATTTATTTTAAATTTGAGTAATTTCAAATTCCACAAACAAAACTGAA
  GAACAGCAATATTTTGTTTGAATTTCTCTCTTCTGTACACTCAGTGATCTAAAACACCAC
  AATATCCAACATACACAAACCTCAGGGAAGGGTTAGTAAACACACACAAGATTGGAATCA
  TGGGGCTCTTTGCTCCTGAATGGAATGGTCCCACAGAAAAAGCACAGGATACAGCACAAC
  ATAAGGGCACCTGTTACATATGAAGTGAGCAAAACATACTAGCATTTTCTATATGCATAA
  TGGGGAAACCTGCATAGGTTAGAGGGCCTTTTACGCTCATTTAAAAATCAGGCAAGTTGT
  CTGTAACATTTTTCAATAATCTGGGAAGCACTGCAATCCAGTGTACGATGTGCTGATTAG
  TGTTGTCTTTGTTGGCACTGACAGTCTTGCATGGTCATATGCCAGTGTTTTTGCTTTAGC
  TTTTCTTTGAATAAAACAGATTTAAATGCATTTAGACAATACGTATTTGTAAGCTGTTTA
  CATACACTAAATTTAAGAGATTCAATATTAGAGTTTCTTTGTTTCTTTAAACACTACAGA
  GTGCAAATCAGGTTCTTCACAACAGATTGAATATTGAGCAGTTCTTTAAAGAAAGAGGGG
  GAAGAAAAAAAGCCCAAGTGAATAAAACATTGAAACTATTCCCCTTCGAAATAAATTCTA
  AATGATGTGGAATGTGAAATAAGGGTTNTAACATAGGTGATCCAAGTTTATAGNTAGNAC
  AAAAAGAAAGTCCTCATGAANTAAGGGTACAGAACACGTTGNCTGTTTTCCCCATTATAC
  TGAGAANTGGTAAGACGATGTTTANTACAAAATAGNGACTACAGATCACGCTCATTCAAT
  GCTGTACCAGGCAGCTGTGCTGNCTAACGGGGGGCGGATACATAACAAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004859
• Insert Size: 6510 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004859.2, NP_004850.1
• RefSeq Size: 6541 bp
• RefSeq ORF: 5028 bp
• Locus ID: 1213
• UniProt ID: Q00610
• Cytogenetics: 17q23.1
• Domains: Clathrin, Clathrin_propel
• Protein Pathways: Endocytosis, Huntington's disease, Lysosome
• Gene Summary: Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter protein (isoform 1).