CDH18 (NM_004934) Human Untagged Clone

CAT#: SC117038

CDH18 (untagged)-Human cadherin 18, type 2 (CDH18), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDH18
• Synonyms: CDH14; CDH14L; CDH24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117038 sequence for NM_004934 edited (data generated by NextGen Sequencing)
  ATGAAAATTACTAGCACATCTTGCATCTGTCCAGTCCTAGTGTGTCTCTGTTTTGTGCAG
  AGGTGTTATGGAACTGCTCACCACAGCTCCATCAAGGTGATGAGAAACCAAACCAAACAC
  ATTGAAGGTGAAACCGAAGTCCATCATCGTCCCAAAAGGGGATGGGTATGGAATCAGTTC
  TTTGTTTTAGAAGAACATATGGGACCAGATCCTCAGTATGTTGGAAAGCTGCACTCCAAT
  TCTGACAAAGGTGATGGATCTGTCAAGTACATCCTTACTGGAGAGGGTGCTGGGACTATA
  TTTATCATTGACGATACCACGGGTGATATCCACTCAACAAAAAGCCTAGACAGAGAGCAG
  AAGACCCACTATGTGCTTCATGCTCAAGCTATTGATAGACGTACAAACAAACCTCTTGAG
  CCTGAATCCGAGTTCATCATCAAAGTGCAAGACATCAATGACAACGCTCCAAAATTCACA
  GATGGACCATACATTGTTACTGTGCCTGAAATGTCAGATATGGGTACCTCTGTTCTACAG
  GTGACAGCTACTGATGCAGATGACCCTACCTATGGAAACAGCGCTCGGGTGGTTTACAGC
  ATTCTCCAGGGACAACCCTACTTCTCCGTCGACCCTAAAACAGGAGTTATTAGAACGGCC
  TTACATAACATGGACAGAGAAGCCAGAGAACATTACTCCGTAGTCATTCAAGCCAAAGAC
  ATGGCTGGGCAAGTTGGAGGGCTTTCAGGATCTACAACAGTCAACATCACCTTAACCGAT
  GTCAATGACAACCCACCACGCTTTCCTCAAAAACACTATCAGCTATATGTTCCTGAGTCA
  GCTCAAGTTGGTTCAGCTGTTGGGAAAATCAAGGCAAATGATGCTGACACTGGCTCAAAT
  GCTGACATGACCTACTCCATCATAAATGGTGATGGCATGGGAATATTCTCAATCTCCACT
  GACAAAGAGACCAGAGAAGGAATCCTTTCTTTAAAGAAGCCACTGAACTATGAGAAAAAG
  AAGTCATATACCCTCAACATAGAAGGAGCAAATACACATCTTGATTTTCGCTTTTCTCAC
  TTGGGTCCTTTTAAAGATGCTACTATGCTGAAGATCATTGTTGGGGATGTAGATGAACCA
  CCACTATTTTCCATGCCTTCCTACCTCATGGAAGTCTACGAAAATGCCAAGATTGGGACC
  GTCGTTGGTACAGTTTTGGCACAAGATCCTGACAGTACTAACAGCTTAGTAAGATACTTC
  ATCAACTACAATGTTGAAGACGACAGATTTTTCAACATTGATGCCAATACTGGGACCATT
  AGGACTACAAAGGTTCTCGACAGAGAAGAAACTCCATGGTACAACATCACAGTCACTGCT
  TCAGAAATTGATAATCCTGATTTGCTGAGCCATGTCACAGTGGGTATTAGAGTTCTGGAT
  GTCAATGACAATCCACCCGAACTTGCCAGGGAATATGATATTATTGTATGTGAAAATTCT
  AAGCCTGGCCAGGTTATTCATACCATCAGTGCCACTGATAAAGATGATTTTGCCAATGGA
  CCAAGGTTTAACTTCTTTCTTGATGAACGCCTGCCTGTAAATCCAAACTTCACTCTGAAG
  GACAATGAAGATAACACAGCCAGCATTCTGACAAGGCGGAGGAGATTTAGTCGAACTGTT
  CAGGATGTGTATTATCTGCCCATTATGATCTCTGATGGTGGAATCCCCTCTCTCAGCAGC
  AGCAGCACCCTCACCATCAGGGTTTGTGCATGCGAGAGAGATGGGCGTGTGCGGACCTGC
  CATGCAGAAGCCTTCCTGTCCTCGGCTGGTTTGAGTACAGGAGCCTTAATCGCTATTCTT
  CTCTGTGTTCTCATTCTCCTGGCAATTGTGGTACTTTTTATCACCCTGAGGCGCAGCAAA
  AAAGAGCCCTTGATCATTTCAGAAGAGGATGTACGGGAGAACGTGGTCACCTATGATGAT
  GAAGGAGGCGGAGAGGAAGACACAGAGGCCTTTGACATCACAGCCTTGAGGAATCCTTCT
  GCTGCTGAGGAGCTCAAGTACCGGAGGGATATCAGACCTGAAGTGAAGCTCACTCCCAGA
  CACCAGACATCATCCACCCTGGAAAGCATAGATGTTCAGGAATTTATTAAGCAAAGACTG
  GCAGAAGCAGACCTAGACCCTAGCGTTCCCCCTTATGACTCTCTTCAGACTTATGCCTAT
  GAGGGTCAGAGATCAGAAGCTGGGTCTATCAGCTCGCTGGATTCAGCAACGACACAATCA
  GACCAGGATTATCACTACCTTGGAGACTGGGGACCCGAGTTTAAAAAGTTAGCTGAACTC
  TATGGAGAAATAGAATCTGAAAGAACAACTTAG
  
  Clone variation with respect to NM_004934.3
  609 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004934 unedited
  GATATTTGTATACGACTTACTATAGGGGCGGCCGCGATTCGGCACGAGGCCGAGGACGGC
  GAGCAGCTGTGCCCAGAGCTCTGGGAAAGTCCCCTCTCTTTGCTTCTTATAAATTAAGTT
  CCTTTCCACCAGTTAGGGCAAGTCCGCCAAGATAGACTGAAGAGGAGCAAGAACAGAAAC
  AGGGAGCCAGTTACAGATCTATCCCAAGAATTCTGGGGAGAGATGATGGTGTCTGCAGCA
  GAGTGACAGCAGTGGAGATTGAGAGAAAAGGTTGAAAATGGTTGCTTGTGTTCAACTACT
  GTGAAGAGTTGGAATCAGACAACTACGGCTTTGATTTTCCAAGTGGTTCAGAATGTAAAT
  ATGATCTGACCTTTCCCTGATGGACAGTTAAATCATGGACACGCTAAACAATTTACTTTG
  GACTGTCCTTCCTAAAGATGCTCTGGCTTCTCTCTTGAATGCTCACTAAGCTTTCGTAGC
  TGACAAGGAAAGGAAGGAAAACTGTGAACTGGAAAGTTATCTTACAATGAAAATTACTAG
  CACATCTTGCATCTGTCCAGTCCTAGTGTGTCTCTGTTTTGTGCAGAGGTGTTATGGAAC
  TGCTCACCACAGCTCCATCAAGGTGATGAGAAACCAAACCAAACACATTGAAGGTGAAAC
  CGAAGTCCATCATCGTCCCAAAGGGGATGGGTATGGAATCAGTTCTTTGGTTAGAAGACA
  TATGGGACCAGATCCTCAGTATGTGGNAAAGCTGCACTCCAATTCTGACNAAGTGGATGA
  ATCTGGTCAGTACCATTCTACTGGGAGAGGGTGGCTGGGACTATATNTATCATTGACGAT
  CCCACGGGTGAATTCCACTCACACAAAGCCTAGACAGAAGCCGAAGACCCCTTTTGGGCT
  CATGCCTCAGCTTTGGTAGACGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004934 unedited
  ACCGCGGGCCGCAATTCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTCTTCCATTAA
  ATAACCCAGTTTCGATCATGAAAAGGGCACTTGTTTCTACAGGAGCTGTGTCCAGCTTCC
  TCAGTTCCAAGTACTGTTTCCACACTTCTTCCTTGTCATTGCTGAGGGTGTATATCCACT
  TACTCAGGAAGCAAATTCCACAAGGTTGCAAGAACTGACCCCCTAAGTTGTTCTTTCAGA
  TTCTATTTCTCCATAGAGTTCAGCTAACTTTTTAAACTCGGGTCCCCAAGTNCTNNCCAA
  GGGTAGTGATANNNNNNNNTTNTTTCCCNNCTNNTTTCTTCTTTNCTTTCTTCTTTTTTC
  GGCCTTTTCCTCTCCTCCCCCCCTCCCCCCCCTTCCCTTCCCCCTCCCCCTCCCTCCCTC
  GCTCCCTTCCTCCCTTCTTTCTCCCTCCCCCTCCTTTCCCCCCCCCCCCCTCCCCCTTTT
  CCTCCCTCTCCCCTCTTCCCTCTTCCCCCTCCTTCCTTTTTCTCCCCTCCCCCCTCCCCC
  TCCTTCCTCCCTCCCTCTCCTCCCTTCCCTTCCTTTCCCCTCCCCCCGTTCCCCTTCCTC
  CCCCTTCTTTTCCCCGTCCCTCTCCTTTCTCCCCCCGCTTTCCTTCTACCTTTTTCCCCT
  CCCTGTCCTTCCTTCCTTCTTCTGCCTCTCCCTCCTGGTCCCGTTTTCCCTTTCCCTCCT
  TTTCTCCCCTTTTCCTTCCCTTCTCTTCTCCTCCGCCCCCATTCCCCATTCGCTTTCATA
  TCACCGCCTACTTCCTCCATTATTTCCCACCCCCTTATTACCCCTCCCCACCCCCCCCTC
  TCATCCCTTTTTTTTTACCCCACCCCCCCCCCCACCCCTATCCCCTATCTTTTTCTTCCC
  CCTCTCTCTCCCTCTCCACTCACTCCCCCCCATCTCTCCCGCCCATGTTCCTATAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004934
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004934.2, NP_004925.1
• RefSeq Size: 2944 bp
• RefSeq ORF: 2373 bp
• Locus ID: 1016
• UniProt ID: Q13634
• Cytogenetics: 5p14.3
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 3, 5, 6, and 7 encode the same protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.