PP4R1 (PPP4R1) (NM_005134) Human Untagged Clone

CAT#: SC116949

PPP4R1 (untagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PP4R1
• Synonyms: MEG1; PP4(Rmeg); PP4R1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005134 edited
  CGGCACCAGCCGCCAGCCCGGCCCTCCTCCTCCCCGCCGCCCCTCCCGCGCGCGTCCACC
  GGCTCCCCGGCTCGCGCCGCTCCATGTAGCCCCGGCTCCCCGGGCGGCCGCGGAGGAGGG
  GGCGACCACAAGATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGAC
  GGATCCCTGGACTTTGTCTCACAAGATGAAATGTTGACGCCCCTGGGGAGATTGGACAAG
  TATGCTGCAAGTGAGAACATATTTAACAGACAAATGGTGGCCCGGAGTTTGCTCGATACC
  TTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTTTTGGAAAGAATTAGCAGA
  TTGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGCCTCACATC
  GCACTGTTTTGTCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTA
  CCTATTGTGGTTAGATACCTTGCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCA
  GCTTTGCTGGCTCTGTTGGAGCAGGAGCTCATTGAACGATTTGATGTGGAGACCAAAGTG
  TGCCCTGTCCTCATAGAGCTGACAGCCCCAGATAGCAATGATGATGTGAAAACAGAAGCT
  GTGGCTATAATGTGCAAAATGGCTCCCATGGTTGGGAAGGATATTACAGAGCGTCTTATC
  CTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTTCACGTTCGAAAGGTCTGT
  GCTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAGAAATGTTG
  CTGCCCAGATTTTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCT
  GAATGCTTCATGGCGGTTTCATGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCA
  GCACTTTTTATTAATTTGATCAGTGATCCTTCACGTTGGGTTCGCCAAGCAGCTTTTCAG
  TCTCTGGGACCTTTCATATCTACTTTTGCTAATCCATCTAGCTCAGGCCAGTATTTTAAA
  GAAGAAAGCAAAAGTTCAGAAGAGATGTCAGTAGAAAACAAAAATAGGACCAGAGATCAA
  GAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCTTCAGATCTCAGTGTTAGT
  AATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCATCCGGGAAG
  CCTCTAGGTGAAATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAA
  TCTCACCAGGAAGCAGCTAGTAATGAGAATGATAAAAAACCTGGTAACTACAAATCTATG
  TTACGACCAGAGGTTGGCACCACTTCACAAGATTCAGCTCTCTTAGATCAGGAATTGTAT
  AACTCCTTCCATTTCTGGAGGACTCCTCTTCCTGAAATAGATCTAGACATAGAGCTTGAA
  CAGAACTCTGGGGGAAAACCCAGCCCAGAGGGACCAGAGGAAGAATCTGAGGGCCCTGTG
  CCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTGGAAGAAATGATAGAAAAT
  CTAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGTCCGCTGCA
  CTACGTGCTTCCAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGAT
  TTGCAAGATGAACTGGATATAAATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCT
  GTGCCTTTAATCAGCGATGCTGTTGAGAATATGGACTCCACTCTTCACTATATTCACAGC
  GATTCAGACTTGAGCAACAATAGCAGTTTTAGCCCTGATGAGGAAAGGAGAACTAAAGTA
  CAAGATGTTGTACCTCAGGCGTTGTTAGATCAGTATTTATCTATGACTGACCCTTCTCGT
  GCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATATAGCCTCCCTGGTGTGGCC
  TTGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTCTGGCCTCA
  GACATGCAGTGGAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATT
  CTTGGAGATCAATTGACAGCTGCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGAC
  CTCGATGAAGTCAGGATAGGTGTTCTTAAACACTTGCATGATTTTCTGAAGCTTCTTCAT
  ATTGACAAAAGAAGAGAATATCTTTATCAACTTCAGGAGTTTTTGGTGACAGATAATAGT
  AGAAATTGGCGGTTTCGAGCTGAACTGGCTGAACAGCTGATTTTACTTCTAGAGTTATAT
  AGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTGAATCTGTGTGCAGACAAA
  GTTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGAAGCTGCAC
  GCGGCAACACCACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGC
  AGATGTCCCAAGTGGTCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAG
  GATGACTGCCTTCCCATGGACCAGTTTGCTGTGCATCTCATGCCGCATCTGCTAACCTTA
  GCAAATGACAGGGTTCCTAACGTGCGAGTGCTGCTTGCAAAGACATTAAGACAAACTCTA
  CTAGAAAAAGACTATTTCTTGGCCTCTGCCAGCTGCCACCAGGAGGCTGTGGAGCAGACC
  ATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTATTTTGCAAGCATCCACCCT
  GCCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTACTAGAAGGCT
  TGAATCTCGGTGTCTTTCCTGCTTCCATGAGAGCCGAGGTTCAGTGGGCATTCGCCACGC
  ATGTGACCTGAGATAGCTTTCGGGGGAGGAGAGACCTTCCTCTCCTGCGGACTTCATTGC
  A
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005134 unedited
  TGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACCAGCCGCCAGCCC
  GGCCCTCCTCCTCCCCGCCGCCCCTCCCGCGCGCGTCCACCGGCTCCCCGGCTCGCGCCG
  CTCCATGTAGCCCCGGCTCCCCGGGCGGCCGCGGAGGAGGGGGCGACCACAAGATGGCGG
  ACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATCCCTGGACTTTGTCT
  CACAAGATGAAATGTTGACGCCCCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACA
  TATTTAACAGACAAATGGTGGCCCGGAGTTTGCTCGATACCTTGAGGGAAGTCTGCGATG
  ATGAAAGAGATTGTATTGCTGTTTTGGAAAGAATTAGCAGATTGGCCGATGATTCAGAAC
  CAACTGTGAGAGCGGAGCTGATGGAACAGGTGCCTCACATCGCACTGTTTTGTCAAGAAA
  ACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACCTATTGTGGTTAGATACC
  TTGCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCTCTGTTGG
  AGCAGGAGCTCATTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGC
  TGACAGCCCCAGATAGCAATGATGATGTGAAAACAGAAGCTGTGGCTATAATGTGCANAA
  TGGCTCCCATGGTTGGGAAGGATATTACANGAGCGTCTATCCTCCCCTAGTTNTGTGAGA
  TGTGCTGCGATTGCAGAATGGTTTCACGTCGAAAGGTCTGTGCTGCCAATTTTGGAGATA
  TTTGCAGTGTAGTTGGCCCAGCAGCTACTGAAGAAATGTTGCTGCCCAGATTTTCCAGNN
  CTTGTCTGATATGTATGGNGAGTCCGAAGGCTGTGCTGATGCTCATGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005134 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTATTAATAA
  ATTTTATTTGGACAAGAGAACTTGTGCCACAACAGTTTAAACAGCATGATTAAAGACTGC
  AGCACTCCCAAGAGTGGTCACAGGTATGTACAGTATGTGAAGAAGTTGCACCTCAGAGCT
  GATCATGATCAAGTTAAAAACACCAGACATACATTATACGTCTAATAAATTTCCTTCAGG
  TCATGACCAGCATGAAAAAATCAGACACAGACACTCAGCAACCATTCTGTAAATGTGTAC
  AGCAATATGCTGCGCTTAAGAGTTTAAGTCAATCCTACTTGTGTTGGCATCAGGTCCTTT
  AGGAGATGTAAAAACCCCTCCTTTCCCATTTGCACACGTCACAAACGATTCACACACAGG
  GCTGGGCTGGACAGCTGGCCACAGAGCCCAGCAAGTCCTTCCTGGGAGAGAAGAGTTAGG
  GCTGATACTGAAGGTCTCTTTCACATCTGGGCACACGTCTGCCTTCAGGCTGTAAGAATT
  TCATTTGTCGATTGTTAAATAAAACCAGGAGAAAGCAATGCAGGTCTCTGGGAATCTCAT
  CCCTTCCATAAGGAAAATGCTCTGCCAATTCAAGTTTCATTCAGTCAGGAAGACAGAAGG
  ATTTAAGGCTTCGGTGACAATTATAATCCTCTGAGAAATATTTCCCCTTAAAGTCAAGAT
  AAGATAATAGTGTTTACTGTACTTTCTCTTGACTCTTGAATCCCTGGTATTGGGTGTAGG
  CAACTTGCACCTGNCATGAAGTCCGCAGGAGAAGAAAGTCTCTCCTCCCCCGAAAGCTAT
  CCCAGTACATGCGTGGCGAATGCCCACTGNACCTCGGCTCCATGGAAGCAGGAAGACCCG
  AGATTCAGCCTCTANTAGGGTGAGACGCGTGCTCATGGCATCTCGAAATTTGTACTGCCA
  GGGGGATGCTGCAAATACTGACTCGCTGCACGGTCATCTGAAGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005134
• Insert Size: 5410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005134.1, NP_005125.1
• RefSeq Size: 3878 bp
• RefSeq ORF: 2802 bp
• Locus ID: 9989
• UniProt ID: Q8TF05
• Cytogenetics: 18p11.22
• Domains: HEAT
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1.This results in a shorter protein (isoform b), compared to isoform a.