CCS (NM_005125) Human Untagged Clone

CAT#: SC116944

CCS (untagged)-Human copper chaperone for superoxide dismutase (CCS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116944 sequence for NM_005125 edited (data generated by NextGen Sequencing)
  ATGGCTTCGGATTCGGGGAACCAGGGGACCCTCTGCACGTTGGAGTTCGCGGTGCAGATG
  ACCTGTCAGAGCTGTGTGGACGCGGTGCGCAAATCCCTGCAAGGGGTGGCAGGTGTCCAG
  GATGTGGAGGTGCACTTGGAGGACCAGATGGTCTTGGTACACACCACTCTACCCAGCCAG
  GAGGTGCAGGCTCTCCTGGAAGGCACGGGGCGGCAGGCGGTACTCAAGGGCATGGGCAGC
  GGCCAGTTGCAGAATCTGGGGGCAGCAGTGGCCATCCTGGGGGGGCCTGGCACCGTGCAG
  GGGGTGGTGCGCTTCCTACAGCTGACCCCTGAGCGCTGCCTCATCGAGGGAACTATTGAC
  GGCCTGGAGCCTGGGCTGCATGGACTCCACGTCCATCAGTACGGGGACCTTACAAACAAC
  TGCAACAGCTGTGGGAATCACTTTAACCCTGATGGAGCATCTCATGGGGGCCCCCAGGAC
  TCTGACCGGCACCGCGGAGACCTGGGCAATGTCCGTGCTGATGCTGACGGCCGCGCCATC
  TTCAGAATGGAGGATGAGCAGCTGAAGGTGTGGGATGTGATTGGCCGCAGCCTGATTATT
  GATGAGGGAGAAGATGACCTGGGCCGGGGAGGCCATCCCTTATCCAAGATCACAGGGAAC
  TCCGGGGAGAGGTTGGCCTGTGGCATCATTGCACGCTCCGCTGGCCTTTTCCAGAACCCC
  AAGCAGATCTGCTCTTGCGATGGCCTCACCATCTGGGAGGAGCGAGGCCGGCCCATCGCT
  GGCAAGGGCCGAAAGGAGTCAGCGCAGCCCCCTGCCCACCTTTGA
  
  Clone variation with respect to NM_005125.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005125 unedited
  GGTGCATTTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCGCTGGTT
  GGTGCTCCTGCGCCGGAGGAGTTCTGCGTCTCGGGGTGGTGACTGGGTCCAGAATGGCTT
  CGGATTCGGGGAACCAGGGGACCCTCTGCCGTTGGAGTTCGCGGTGCAGATGACCTGTCA
  GAGCTGTGTGGACGCGGTGCGCAAATCCCTGCAAGGGGTGGCAGGTGTCCAGGATGTGGA
  GGTGCACTTGGAGGACCAGATGGTCTTGGTACACACCACTCTACCCAGCCAGGAGGTGCA
  GGCTCTCCTGGAAGGCACGGGGCGGCAGGCGGTACTCAAGGGCATGGGCAGCGGCCAGTT
  GCAGAATCTGGGGGCAGCAGTGGCCATCCTGGGGGGGCCTGGCACCGTGCAGGGGGTGGT
  GCGCTTCCTACAGCTGACCCCTGAGCGCTGCCTCATCGAGGGAACTATTGACGGCCTGGA
  GCCTGGGCTGCATGGACTCCACGTCCATCAGTACGGGGACCTTACAAACAACTGCAACAG
  CTGTGGGAATCACTTTAACCCTGATGGAGCATCTCATGGGGGCCCCCAGGACTCTGACCG
  GCACCGCGGAGACCTGGGCAATGTCCGTGCTGATGCTGACGGCCGCGCCATCTTCAGAAT
  GGAGGATGAGCAGCTGAAGGTGTGGGATGTGATTGGCCGCAGCCTGATTATTGATGANGG
  AGAAGATGACCTGGGCCGGGGAGGCCATCCCTTATCCCAGATCACAGGGAACTCCGGGGA
  GAGGGTTGCCCTGTGGCATCATTGCACGCTCCGCTGGCCTTTTCCAGAACCCCAGCAGAT
  CTGTTCTTGCGATGGCCTCACCTCTGGGGAGGACCGAGGCCGCCCATCGCTTGCCAGGCC
  CCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005125 unedited
  CGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCCAAACGAAAATAAAACTTTCATT
  TGCCAAGGGAACACCACAGCAGCTCCTGCCCTGTACTGAGCTCTCCAAAGACTGGGCAGG
  CAAAGTCCCTCTGGCCCCCTCTGGAAGTGGAAAGTGCTCGCCCTGGAGGACAGCAACAGA
  GCCAAGGTGAGGTCCTGCTCAAAGGTGGGCAGGGGGCTGCGCTGACTCCTTTCGGCCCTT
  GCCAGCGATGGGCCGGCCTCGCTCCTCCCAGATGGTGAGGCCATCGCAAGAGCAGATCTG
  CTTGGGGTTCTGGAAAAGGCCAGCGGAGCGTGCAATGATGCCACAGGCCAACCTCTCCCC
  GGAGTTCCCTGTGATCTTGGATAAGGGATGGCCTCCCCGGCCCAGGTCATCTTCTCCCTC
  ATCAATAATCAGGCTGCGGCCAATCACATCCCACACCTTCAGCTGCTCATCCTCCATTCT
  GAAGATGGCGCGGCCGTCAGCATCAGCACGGACATTGCCCAGGTCTCCGCGGTGCCGGTC
  AGAGTCCTGNGGGCCCCCATGAGATGCTCCATCAGGGTTAAAGTGATTCCCACAGCTGTT
  GCAGTTGTTTGTAAGGTCCCCGTACTGATGGACGTGGAGTCCATGCAGCCCAGGCTCCAG
  GCCGTCAATAGTTCCCTCGATGAGGCAGCGCTCAGGNGTCAGCTGTANGAAGCGCACCAC
  CCCCTGCACGGTCCAGGCCCCCCCCAGATGCCACTGCTGCCCCAGATTCTGCACTGGCCG
  CTGCCCATGCCCTTGAGTACGCCTGCCCGCCGTGCCTTNCAGAGAGCTGCACTNCTGGCT
  GGTAAAGTGTGTGTACCAGACATCTGTTCTCCAGTGCACTNCACATCTGNCACCTGCCAC
  CCTGCAGGGATTGCCCCCGTCCACATCTTGAAGTATTTGCCCGGACTCACGGCAAAGTCC
  CTGGTTCCGATCGACCATTTGACATCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005125
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005125.1, NP_005116.1
• RefSeq Size: 1068 bp
• RefSeq ORF: 825 bp
• Locus ID: 9973
• UniProt ID: O14618
• Cytogenetics: 11q13.2
• Domains: sodcu, HMA
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: Copper chaperone for superoxide dismutase specifically delivers Cu to copper/zinc superoxide dismutase and may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor. [provided by RefSeq, Jul 2008]