c Fos (FOS) (NM_005252) Human Untagged Clone

CAT#: SC116873

FOS (untagged)-Human FBJ murine osteosarcoma viral oncogene homolog (FOS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: c Fos
• Synonyms: AP-1; C-FOS; p55
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005252 edited
  GAATTCGGCACGAGGTGCCTAACCGCCACGATGATGTTCTCGGGCTTCAACGCAGACTAC
  GAGGCGTCATCCTCCCGCTGCAGCAGCGCGTCCCCGGCCGGGGATAGCCTCTCTTACTAC
  CACTCACCCGCAGACTCCTTCTCCAGCATGGGCTCGCCTGTCAACGCGCAGGACTTCTGC
  ACGGACCTGGCCGTCTCCAGTGCCAACTTCATTCCCACGGTCACTGCCATCTCGACCAGT
  CCGGACCTGCAGTGGCTGGTGCAGCCCGCCCTCGTCTCCTCTGTGGCCCCATCGCAGACC
  AGAGCCCCTCACCCTTTCGGAGTCCCCGCCCCCTCCGCTGGGGCTTACTCCAGGGCTGGC
  GTTGTGAAGACCATGACAGGAGGCCGAGCGCAGAGCATTGGCAGGAGGGGCAAGGTGGAA
  CAGTTATCTCCAGAAGAAGAAGAGAAAAGGAGAATCCGAAGGGAAAGGAATAAGATGGCT
  GCAGCCAAATGCCGCAACCGGAGGAGGGAGCTGACTGATACACTCCAAGCGGAGACAGAC
  CAACTAGAAGATGAGAAGTCTGCTTTGCAGACCGAGATTGCCAACCTGCTGAAGGAGAAG
  GAAAAACTAGAGTTCATCCTGGCAGCTCACCGACCTGCCTGCAAGATCCCTGATGACCTG
  GGCTTCCCAGAAGAGATGTCTGTGGCTTCCCTTGATCTGACTGGGGGCCTGCCAGAGGTT
  GCCACCCCGGAGTCTGAGGAGGCCTTCACCCTGCCTCTCCTCAATGACCCTGAGCCCAAG
  CCCTCAGTGGAACCTGTCAAGAGCATCAGCAGCATGGAGCTGAAGACCGAGCCCTTTGAT
  GACTTCCTGTTCCCAGCATCATCCAGGCCCAGTGGCTCTGAGACAGCCCGCTCCGTGCCA
  GACATGGACCTATCTGGGTCCTTCTATGCAGCAGACTGGGAGCCTCTGCACAGTGGCTCC
  CTGGGGATGGGGCCCATGGCCACAGAGCTGGAGCCCCTGTGCACTCCGGTGGTCACCTGT
  ACTCCCAGCTGCACTGCTTACACGTCTTCCTTCGTCTTCACCTACCCCGAGGCTGACTCC
  TTCCCCAGCTGTGCAGCTGCCCACCGCAAGGGCAGCAGCAGCAATGAGCCTTCCTCTGAC
  TCGCTCAGCTCACCCACGCTGCTGGCCCTGTGAGGGGGCAGGGAAGGGGAGGCAGCCGGC
  ACCCACAAGTGCCACTGCCCGAGCTGGTGCATTACAGAGAGGAGAAACACATCTTCCCTA
  GAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAACACACCAGGCTGTGGG
  CCTCAAGGACTTGAAAGCATCCATGTGTGGACTCAAGTCCTTACCTCTTCCGGAGATGTA
  GCAAAACGCATGGAGTGTGTATTGTTCCCAGTGACACTTCAGAGAGCTGGTAGTTAGTAG
  CATGTTGAGCCAGGCCTGGGTCTGTGTCTCTTTTCTCTTTCTCCTTAGTCTTCTCATAGC
  ATTAACTAATCTATTGGGTTCATTATTGGAATTAACCTGGTGCTGGATATTTTCAAATTG
  TATCTAGTGCAGCTGATTTTAACAATAACTACTGTGTTCCTGGCAATAGTGTGTTCTGAT
  TAGAAATGACCAATATTATACTAAGAAAAGATACGACTTTATTTTTTGGTAGATAGAAAT
  AAATAGCTATATCCATGTACTGTAGTTTTTCTTCAACATCAATGTTCATTGTAATGTTAC
  TGATCATGCATTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG
  TTTTATTGTGTTTTTAATTTATTTATTAAGATGGATTCTCAGATATTTATATTTTTATTT
  TATTTTTTTCTACCTTGAGGTCTTTTGACATGTGGAAAGTGAATTTGAATGAAAAATTTA
  AGCATTGTTTGCTTATTGTTCCAAGACATTGTCAATAAAAGCATTTAAGTTGAATGCGAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005252 unedited
  CTTGGATTTGTAACCGACTTTATATAGGCGGCCGCGCAATTCGCACGAGGTGCCTAACCG
  CCACGATGATGTTCTCGGGCTTCAACGCAGACTACGAGGCGTCATCCTCCCGCTGCAGCA
  GCGCGTCCCCGGCCGGGGATAGCCTCTCTTACTACCACTCACCCGCAGACTCCTTCTCCA
  GCATGGGCTCGCCTGTCAACGCGCAGGACTTCTGCACGGACCTGGCCGTCTCCAGTGCCA
  ACTTCATTCCCACGGTCACTGCCATCTCGACCAGTCCGGACCTGCAGTGGCTGGTGCAGC
  CCGCCCTCGTCTCCTCTGTGGCCCCATCGCAGACCAGAGCCCCTCACCCTTTCGGAGTCC
  CCGCCCCCTCCGCTGGGGCTTACTCCAGGGCTGGCGTTGTGAAGACCATGACAGGAGGCC
  GAGCGCAGAGCATTGGCAGGAGGGGCAAGGTGGAACAGTTATCTCCAGAAGAAGAAGAGA
  AAAGGAGAATCCGAAGGGAAAGGAATAAGATGGCTGCAGCCAAATGCCGCAACCGGAGGA
  GGGAGCTGACTGATACACTCCAAGCGGAGACAGACCAACTAGAAGATGAGAAGTCTGCTT
  TGCAGACCGAGATTGCCAACCTGCTGAAGGAGAAGGAAAAACTAGAGTTCATCCTGGCAG
  CTCACCGACCTGCCTGNCAGATCCCTGATGACCTGNGCTTCCCAGAAGAGATGTCTGTGG
  CTTCCCTTGATCTGACTGGNGGCCTGCCAGAAGTTGCCACCCCGGAGTCTGNNAGAGCCT
  TCACCCTGCCTCTCCCTCATGACCCTGAGCCCCAGCCCTCAGTGGAACCCTGTCAGAGCA
  TCAGCAGCATGGAGCTGAAGACCGAGCCCTTTGATGACTTTCTGGTCCCAGCATCATCCA
  GGCCCAATGGCTCTGAAACAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005252 unedited
  CACTTAAATAGCTTTTTTGACATGTCTTGGNACAATAAGCAAACAATGCTTAAATTTTTC
  ATTCAAATNACTTTCCACATGTCAAAAGACCTCAAGGTAGAAAAAAATAAAATAAAAATA
  TAAATATCTGAGAATCCATCTTAATAAATAAATTAAAAACACAATAAAACGTTTTCATGG
  AAAACTGTTAATGTCAGAACATTCAGACCACCTCAACAATGCATGATCAGTAACATTACA
  ATGAACATTGATGTTGAAGAAAAACTACAGTACATGGATATAGCTATTTATTTCTATCTA
  CCAAAAAATAAAGTCGTATCTTTTCTTAGTATAATATTGGTCATTTCTAATCAGAACACA
  CTATTGCCAGGAACACAGTAGTTATTGTTAAAATCAGCTGCACTAGATACAATTTGAAAA
  TATCCAGCACCAGGTTAATTCCAATAATGAACCCAATAGATTAGTTAATGCTATGAGAAG
  ACTAAGGAGAAAGAGAAAAGAGACACAGACCCAGGCCTGGCTCAACATGCTACTAACTAC
  CAGCTCTCTGAAGTGTCACTGGGAACAATACACACTCCATGCGTTTTGCTACATCTCCGG
  AAGATGTAAAGACTTGAGTCCACACATGGATGCTTTCAAGTCCTTGAGGCCCACAGCCTG
  GTGTGTTTCACGCACAGATAATGTCCTCCCTATGTCTACAGGAACCCTCTAGGGAAGATG
  TGTTTCTCCTCTCTGTAATGCACCAGCTCGGGCAGTGGCACTTGTAGGTGCCAGCTGCCT
  CCNCTTNCCCTGCCCCTCACATGCCANCATCGTGGGTGAGCTGAGCGAGTCANAAGAATG
  CTCATTGCTGCTGCTGCCCTTGCNGTGGGCATCTGCACAGCTGGGGAAAGAGTCANCCTC
  GGGGGTAGTGAAAACCAATGGAGACGTGTTATCAGTGCAGCTGGGAGTACAGTGACCACC
  GNATGCCAAGGNGCTCACTCTGTGCCATGGGCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005252
• Insert Size: 2190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: A TrueClone.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005252.2, NP_005243.1
• RefSeq Size: 2084 bp
• RefSeq ORF: 1143 bp
• Locus ID: 2353
• UniProt ID: P01100
• Cytogenetics: 14q24.3
• Domains: BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Colorectal cancer, MAPK signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]