PAR2 (F2RL1) (NM_005242) Human Untagged Clone

CAT#: SC116867

F2RL1 (untagged)-Human coagulation factor II (thrombin) receptor-like 1 (F2RL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAR2
• Synonyms: GPR11; PAR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116867 sequence for NM_005242 edited (data generated by NextGen Sequencing)
  ATGCGGAGCCCCAGCGCGGCGTGGCTGCTGGGGGCCGCCATCCTGCTAGCAGCCTCTCTC
  TCCTGCAGTGGCACCATCCAAGGAACCAATAGATCCTCTAAAGGAAGAAGCCTTATTGGT
  AAGGTTGATGGCACATCCCACGTCACTGGAAAAGGAGTTACAGTTGAAACAGTCTTTTCT
  GTGGATGAGTTTTCTGCATCTGTCCTCACTGGAAAACTGACCACTGTCTTCCTTCCAATT
  GTCTACACAATTGTGTTTGTGGTGGGTTTGCCAAGTAACGGCATGGCCCTGTGGGTCTTT
  CTTTTCCGAACTAAGAAGAAGCACCCTGCTGTGATTTACATGGCCAATCTGGCCTTGGCT
  GACCTCCTCTCTGTCATCTGGTTCCCCTTGAAGATTGCCTATCACATACATGGCAACAAC
  TGGATTTATGGGGAAGCTCTTTGTAATGTGCTTATTGGCTTTTTCTATGGCAACATGTAC
  TGTTCCATTCTCTTCATGACCTGCCTCAGTGTGCAGAGGTATTGGGTCATCGTGAACCCC
  ATGGGGCACTCCAGGAAGAAGGCAAACATTGCCATTGGCATCTCCCTGGCAATATGGCTG
  CTGATTCTGCTGGTCACCATCCCTTTGTATGTCGTGAAGCAGACCATCTTCATTCCTGCC
  CTGAACATCACGACCTGTCATGATGTTTTGCCTGAGCAGCTCTTGGTGGGAGACATGTTC
  AATTACTTCCTCTCTCTGGCCATTGGGGTCTTTCTGTTCCCAGCCTTCCTCACAGCCTCT
  GCCTATGTGCTGATGATCAGAATGCTGCGATCTTCTGCCATGGATGAAAACTCAGAGAAG
  AAAAGGAAGAGGGCCATCAAACTCATTGTCACTGTCCTGGCCATGTACCTGATCTGCTTC
  ACTCCTAGTAACCTTCTGCTTGTGGTGCATTATTTTCTGATTAAGAGCCAGGGCCAGAGC
  CATGTCTATGCCCTGTACATTGTAGCCCTCTGCCTCTCTACCCTTAACAGCTGCATCGAC
  CCCTTTGTCTATTACTTTGTTTCACATGATTTCAGGGATCATGCAAAGAACGCTCTCCTT
  TGCCGAAGTGTCCGCACTGTAAAGCAGATGCAAGTATCCCTCACCTCAAAGAAACACTCC
  AGGAAATCCAGCTCTTACTCTTCAAGTTCAACCACTGTTAAGACCTCCTATTGA
  
  Clone variation with respect to NM_005242.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005242 unedited
  TCTCTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCTCCTTCGGTTTCCCTGAAACCTA
  ACCCGCCCTGGGGAGGCGCGCAGCAGAGGCTCCGATTCGGGGCAGGTGAGAGGCTGACTT
  TCTCTCGGTGCGTCCAGTGGAGCTCTGAGTTTCGAATCGGTGGCGGCGGATTCCCCGCGC
  GCCCGGCGTCGGGGCTTCCAGGAGGATGCGGAGCCCCAGCGCGGCGTGGCTGCTGGGGGC
  CGCCATCCTGCTAGCAGCCTCTCTCTCCTGCAGTGGCACCATCCAAGGAACCAATAGATC
  CTCTAAAGGAAGAAGCCTTATTGGTAAGGTTGATGGCACATCCCACGTCACTGGAAAAGG
  AGTTACAGTTGAAACAGTCTTTTCTGTGGATGAGTTTTCTGCATCTGTCCTCACTGGAAA
  ACTGACCACTGTCTTCCTTCCAATTGTCTACACAATTGTGTTTGTGGTGGGTTTGCCAAG
  TAACGGCATGGCCCTGTGGGTCTTTCTTTTTCGAACTAAGAAGAAGCACCCTGCTGTGAT
  TTACATGGCCAATCTGGCCTTGGCTGACCTCCTCTCTGTCATCTGGCTCCCCTTGAAGAT
  TGCCTATCACATACATGGNCACCAACTGGATTTATGCGCGAAGCTCTTTGTAATGTGCTT
  ATTGGCTTTCTCTATGGCACCCTGTACCTGTTCCATTCTCTTCATGACCTGCCTCAGTGT
  GCAGAGGTATTGGGTCCTTCTGGAACCCCCATGGGGCACTTCACGNAGAAAGCCAACATT
  GCCCTTGGCATCTCCCTGCCAATATGGCCGCTTGATTCTGCTGGGCACCATCCCTTGGGA
  TGTCCGGAAGCAGACCATTTNATTTCTGCCCTGAACATAACGACCTGTATGATGTTTTGC
  CTGACCACTTCTGTGGAAGAACAGTCAAATACTTCTTTCTTTGGCAACTGGGGCCTTTTG
  TTCCAACCTTTCTCACCCCTGCCTATGCCGGAGACACAAAGCGTGGATCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005242 unedited
  TTGGCCGCGGCCGCATTTTANATCGAGTTTTTTTTTTTTTTTTTTAAGTTGTAAAAAAAT
  TTTGTTATTTATAAATCAATTACACAATAAAATAATTATTTTTAAAAGTCACAAATACAA
  TCGTTGTATAAAGTCATTTTGGCATCAAGTATCTCTTAAATATGTTGCAAACTATTTTCC
  AAAGAGATGTGGTCCAAACCCTCTGAAGGCTTTATAATTTTGTATTAGATAACAAGTGAA
  CAAAACTGACAATAAATACTCCAACAATTATTTTTTAAAATACTAAGTGGCAAATGCTAT
  TCTAAGTGGCAAAACAATCTATTACTCAGACTACCTGAAAATTTCACTGAAGTCATCAAA
  AGTTATACAAAATTTGTATTTACATGTTTAAAATCCTGATTGCATTTTTCTTTAATAATA
  ATACATACAAAAACTCAGAGGGTTAATAAAGAAATAATTCAAAGTCCTAATAAGTCAACA
  AACAGATTTCATTATAAGCTGAACATAAAAGAGACACCATTGTTTGCTTCTCTTTTCAAA
  AATTATCACGGCACTTTGTCAAATGGAAGCAGCATTCAGAACAATGGTTCTCANATCTCT
  GTGTGCATAAACACCATGCAGGTTTGTACCACACAGATTCCTGNGCCCTGTTCCCAGTCT
  GAGCTCTCATTATTGAATCTGAGGAATCTGCATTNTGAAACAAGATCACGACTATTTTAT
  TTTATTTTATCACCCACGCTGGAGTGCAGTGGTGCAATCTCGGTTCACTGCACCCTCTGC
  CTCCTGAGTTAAAGTGATACTCAATTCTTGTGCCTCAGCCTCCTGAGTAACTGGGATTAC
  AAACGTGCACCACACACCTGGTTAACTTTTGTATTTTAGNAAAGATGAGTTTCCCCATGA
  TGCCAGCCTGGCCTCGACTCCTGACTCAGTGATGCCTGCCTCACCTCCCAAGGCTAGGAT
  ACAGCTGAGCACCGACCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005242
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005242.3, NP_005233.3
• RefSeq Size: 2876 bp
• RefSeq ORF: 1194 bp
• Locus ID: 2150
• UniProt ID: P55085
• Cytogenetics: 5q13.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family of proteins. The encoded cell surface receptor is activated through proteolytic cleavage of its extracellular amino terminus, resulting in a new amino terminus that acts as a tethered ligand that binds to an extracellular loop domain. Activation of the receptor has been shown to stimulate vascular smooth muscle relaxation, dilate blood vessels, increase blood flow, and lower blood pressure. This protein is also important in the inflammatory response, as well as innate and adaptive immunity. [provided by RefSeq, Jun 2016]