ELK3 (NM_005230) Human Untagged Clone

CAT#: SC116859

ELK3 (untagged)-Human ELK3, ETS-domain protein (SRF accessory protein 2) (ELK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELK3
• Synonyms: ERP; NET; SAP-2; SAP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116859 sequence for NM_005230 edited (data generated by NextGen Sequencing)
  ATGGAGAGTGCAATCACGCTGTGGCAGTTCCTGTTGCAGTTGCTGCTGGATCAGAAACAT
  GAGCATTTGATCTGCTGGACCTCGAACGATGGTGAATTCAAGCTCCTCAAAGCAGAAGAA
  GTGGCCAAGCTGTGGGGACTCCGAAAAAACAAAACAAATATGAACTATGATAAGCTGAGC
  AGAGCCCTGCGATACTATTATGACAAGAACATCATCAAGAAGGTGATCGGGCAGAAGTTT
  GTGTACAAGTTTGTCTCTTTCCCGGAGATCCTGAAGATGGATCCTCACGCGGTGGAGATC
  AGCCGGGAGAGCCTTCTGCTGCAGGACAGCGACTGCAAGGCGTCTCCGGAGGGCCGCGAG
  GCCCACAAACACGGCCTGGCCGCCCTCAGAAGCACGAGCCGCAACGAATACATCCACTCA
  GGCCTGTACTCGTCCTTCACCATTAATTCCCTGCAGAACCCACCAGACGCCTTCAAGGCC
  ATCAAGACGGAGAAGCTGGAGGAGCCGCCCGAAGACAGCCCCCCCGTGGAAGAAGTCAGG
  ACTGTGATCAGGTTTGTGACCAATAAAACCGACAAGCACGTCACCAGGCCGGTGGTGTCC
  CTGCCTTCCACGTCAGAGGCTGCGGCGGCGTCCGCCTTCCTGGCCTCGTCCGTCTCGGCC
  AAGATCTCCTCTTTAATGTTGCCAAACGCTGCCAGTATTTCATCCGCCTCACCCTTCTCA
  TCTCGGTCCCCGTCCCTGTCCCCCAACTCACCCCTCCCTTCTGAACACAGAAGCCTCTTC
  CTGGAGGCCGCCTGCCATGACTCCGATTCCCTGGAGCCCTTGAACCTGTCATCGGGCTCC
  AAGACCAAGTCTCCATCTCTTCCCCCAAAGGCCAAAAAACCCAAAGGCTTGGAAATCTCA
  GCGCCCCCGCTGGTGCTCTCCGGCACCGACATCGGCTCCATCGCCCTCAACAGCCCAGCC
  CTCCCCTCGGGATCCCTCACCCCAGCCTTCTTCACCGCACAGACACCAAATGGATTGCTT
  CTGACTCCGAGTCCACTGCTCTCCAGCATACATTTCTGGAGCAGCCTTAGTCCAGTTGCT
  CCGCTGAGTCCTGCCAGGCTGCAAGGGCCAAGCACGCTGTTCCAGTTCCCCACACTGCTT
  AATGGCCACATGCCAGTGCCAATCCCCAGTCTGGACAGAGCTGCTTCTCCAGTACTGCTT
  TCTTCAAACTCTCAGAAATCCTGA
  
  Clone variation with respect to NM_005230.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005230 unedited
  NGGTCATTCCGGCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
  TAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGC
  CGCGAATTCGGCACGAGGCAGCCGCGGCCGGGGCGCGCGGCGCGGGGCGGAAAAGCCTGT
  TTACACAGACTGCACACCGCCTGGGGAATAATGCAGTAAAGGAAGTGAGCCGGCTCGGCC
  TGACTGCTCCAACTTCCTGCTCTCACACACACCAGAGGGGAAAAAAAAAGAGGAGCGAGA
  GAAAGAAAAAAAGGGGGAAAAATCAGGATCTCATTACAAGAGCCACAGACCGTCTGCAGA
  CGCCTGTCAGCATGGAAAGTCGGGGGCTTTCGCCCGGGTCCTCCTAGAAATTCCCCCCGA
  AGAAGACTCCCCCACATCTGGGTATGGAGAGTGCAATCACGCTGTGGCAGTTCCTGTTGC
  AGTTGCTGCTGGATCAGAAACATGAGCATTTGATCTGCTGGACCTCGAACGATGGTGAAT
  TCAAGCTCCTCAAAGCAGAAGAAGTGGCCAAGCTGTGGGGACTCCGAAAAAACAAAACAA
  ATATGAACTATGATAAGCTGAGCAGAGCCCTGCGATACTATTATGACAAGAACATCATCA
  AGAAGGTGATCGGGCAGAAGTTTGTGTACAAGTTTGTCTCTTTCCCGGAGATCCTGAAGA
  TGGATCCTCACGCGGTGGAGATCAGCCGGGAGAGCCTTCTGCTGCAGTACAGCGACTGCA
  AAGCGTTCTCCGAAGGCCCGCGAGCCCAACAACACGGCCCTGTCCTCCTTCAAAGCACCA
  ACCGCCACGAATTCTTCCACTCAGGCCTGTACTCGTTCCTTACCATTAATTTCCTGCGGA
  ACCACCAGAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005230 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATACAATGTTTATTGTCTAAATATG
  GCTTAGTTTATTATAAAAACATTACTTGGACATTTACTGAATTTCAGTTCAAAAGAGGTT
  CTAAGGCAATTCTTATACAATTGAAAGTAAAACAAAACGTGATATATGCTTTCATATTGC
  CCACTGGTGAAAAATAATGAAATTCTACTAGAGAGCTGTTTGATACTAATTTTCATCTTT
  CAAACATTTCCTCTTAATACTAAAAAATATATAAATCATTCATTTACATTAAATGCGAAG
  GAAGGCACAACTGGTTATAGTCTATATAAGCACACAGTTTATCATACTCAGAACTAAAGA
  GAAAAAAGCTTCACATCCTTATTAGTTATACTGAAAACCATTAAGAAAAGTTCTTCACTG
  TCAACAGTGTTCAACACTTAAGAGCTAAAAGGATATAAAAACAAAAACATTAAAATTCAC
  TTAATGCCAAACAGATTTTCATATATACTTGAATATATCAAAAACAGTTTTTAAAATGCT
  AACATAAATCTAGACTATCCATGTTATGAAAAGTGCAAACCAAATTAACAAGAGTTTCAC
  AATGTCCTTCTCACGACACAGGTAAACTAGCCCGTGGGGACAAATTTGTTTCATCAGTTA
  ATGAGTCCTTAATTGTGGCCAGACGTCATCAGGATTTCTGAGAGTTTGAAGAAAGCAGTA
  CTGGAGAATCAGCTCTGTCCAGACTGGNGATTGGCACTGGCATGTGGCCATTAGCAGTGT
  GGGGAACTGGGACAGCGTGCTTGGCCCTTGCAGCCTGNCAGGACTCANCGGAGGCACTGG
  ACTATAGCTGCTNCANAATAGTATGCTGGAAATCACTGGACCTCGAGTCAAAATCATTCC
  TTTGTGTCTGGCCGGGAATAAGCTGGGTGAGGTATCCCAAGGAGGGCTGGCTTTAAGGCC
  AGAACCCATTCCGTCCCGAAACACCCCGGGGCCCTAATTTCAACCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005230
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005230.2, NP_005221.2
• RefSeq Size: 2180 bp
• RefSeq ORF: 1224 bp
• Locus ID: 2004
• UniProt ID: P41970
• Cytogenetics: 12q23.1
• Domains: ETS
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.