RAB8A (NM_005370) Human Untagged Clone

CAT#: SC116777

RAB8A (untagged)-Human RAB8A, member RAS oncogene family (RAB8A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB8A
• Synonyms: MEL; RAB8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116777 sequence for NM_005370 edited (data generated by NextGen Sequencing)
  ATGGCGAAGACCTACGATTACCTGTTCAAGCTGCTGCTGATCGGGGACTCGGGGGTGGGG
  AAGACCTGTGTCCTGTTCCGCTTCTCCGAGGACGCCTTCAACTCCACTTTTATCTCCACC
  ATAGGAATTGACTTTAAAATTAGGACCATAGAGCTCGATGGCAAGAGAATTAAACTGCAG
  ATATGGGACACAGCCGGTCAGGAACGGTTTCGGACGATCACAACGGCCTACTACAGGGGT
  GCAATGGGCATCATGCTGGTCTACGACATCACCAACGAGAAGTCCTTCGACAACATCCGG
  AACTGGATTCGCAACATTGAGGAGCACGCCTCTGCAGACGTCGAAAAGATGATACTCGGG
  AACAAGTGTGATGTGAATGACAAGAGACAAGTTTCCAAGGAACGGGGAGAAAAGCTGGCC
  CTCGACTATGGAATCAAGTTCATGGAGACCAGCGCGAAGGCCAACATCAATGTGGAAAAT
  GCATTTTTCACTCTCGCCAGAGATATCAAAGCAAAAATGGACAAAAAATTGGAAGGCAAC
  AGCCCCCAGGGGAGCAACCAGGGAGTCAAAATCACACCGGACCAGCAGAAGAGGAGCAGC
  TTTTTCCGATGTGTTCTTCTGTGA
  
  Clone variation with respect to NM_005370.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005370 unedited
  CCTTGGCGACACTGCACCACAATTTTATATAGGGTGGNCGNGNAATTCGCACCAGGCTCT
  CCTTTTTGCCGGCCAATGGGAGGTGCGCTTGGGATGATTAAGGATCCGCGCTCCGATCGC
  CCCCTCCCAGGTCTCCCCGTGACTGGTGGGACGCAGGGGCGGGCGTCGGCCGCGGTGACG
  CGCAGCGGGCCTGGAGCCAATAAGGGGCGGTTTTTTTTGCTGATGGACGGTGCTGGTGGC
  CAGTGGAGAGGCGCTGGCTGCACTTACCGTCGGGGAGAGAGTGTCATATGGTGAAGACCT
  ACGATTACCTGTTCAAGCTGCTGATGATCGGGGACTCGGTGGTGGGGAAGACCTGTGTCC
  TGTTTCGATTCTCCGAGGACGTCTTCAACTCCACTTTTATCTCCACTATAGGAATTGATT
  TTAAAATTAGGAGCATAGAGCTCGATGGCAAAAGAATTAAACTGTAGATATGGGACACAG
  CTGGTCTGGAACGGTTTCGGACGATCACACTGGACTACTACAGGGGTGCAATGGGCATCA
  TGTTGGTCTACGAAATTATCAACGTAGAGTTCTTCCCCAACATCCGGAACTGGATTTCAA
  CATTGAGGAGTCCGCCTCTGCAGACGTCTTAAAGATGAATCCTCGGCACCATCTGTGACG
  TCACTGACCAACAAACATGTTTCTTAGGACACGGTGAAAAACGCTGGGTTTTTAATTATG
  GTATCAGTTTATGGTGACCACCTCCAAGGTCCCACATTACTGTGGCACAGTGATTTTTCA
  TTTCTTTTCTCAGATATCATAGCTAATAGGCCCAAAAATTGGGAAGTACCATCCTCCTAG
  GGGTGCCACCTTGGAGTTCAAACTTCCATGAACCTCCTACAAAGGAGCTCTTTTTTCACA
  GTGTTCTTTTCGTAGCACCACCCTTATTTTGCGCTCCCCACCCTAATGATTGCCTCGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005370 unedited
  TGAGGCAGGGGTACACAGGGCATGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACC
  GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGACTTTAAA
  ATGAGCTTTAATTTGTTTGTTTTCCTTAAGTGCATTTATCGATGTAAACAGAGCATGTTA
  GATATTTTTGGGGAGAGCAGACTATTGCTGATATGTTGGTTTTAATTCAAAAGAAACGAT
  GATGCCTATGGTTTGGATTGACAGCCGCTTAAGGAGGAAAAAAAAAAAAAGAAAGAAAAA
  AAGAAAGGGGTGGTGACACCCCCCTGGCGTGGTTGACCTGGTCCCAGTCGCAGTCCCTAT
  GGGACAGCAGATCTGTCAAAACAGAACCCAATGATGACGGCTGAAAATCAAGCCAGACTG
  CTGTGCGGTCAGCTTGCCGCCGTGGAATGCCTTGTGTCTTGGCCATGTCCAGCCAAGGGT
  ACAACGTGCTTGTTCCCGATCACCCAGGTTTGCCATTGGAAGTCAAAGACAGAATCGCTT
  CATGGCACTTCAGATGTGGAAAAATAAAAATCTCATCTAAAAAGAACGTCCGATTTGGAG
  ATAGCGGGAGGACACGAAGGAGTGGGGGCCATTTTGGTGCTGAGAGGAGGGTGCCCCAAC
  TTCAAGAAGACCATGTGACGGCTGTGGTTGTTTTCGGGGTCATTTGAAACACACACAGCG
  TCCCCTTGATGCTCGATGGGGACCGCAAACGGGCCTGCANACCTAACCCCTGTCTGTGGA
  CAAGAGAGGCTGCTCAAGGCTTCTTTCCTTCTAATGCTTTACACAGGATTCCCTCTTTGT
  GGCTTTCTTTGGTTTACAATAAAAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005370
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005370.4, NP_005361.2
• RefSeq Size: 2194 bp
• RefSeq ORF: 624 bp
• Locus ID: 4218
• UniProt ID: P61006
• Cytogenetics: 19p13.11
• Domains: ras, RAN, RAS, RHO, RAB, ARF
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]