YES1 (NM_005433) Human Untagged Clone

CAT#: SC116734

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YES1 (untagged)-Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)



  "NM_005433" in other vectors (7)

Reconstitution Protocol

USD 506.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol YES1
Synonyms c-yes; HsT441; P61-YES; Yes
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116734 sequence for NM_005433 edited (data generated by NextGen Sequencing)
ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAAT
ACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCA
CCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACA
CCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTG
GTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATA
ATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT
TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTT
GGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGT
ATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGT
GGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTAC
ACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCT
CAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG
GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAA
GCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCT
GAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTT
AAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATT
GCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA
ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCT
CCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATT
CTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAA
GTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAA
TATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGA
GAAAATTTATAA

Clone variation with respect to NM_005433.3
>OriGene 5' read for NM_005433 unedited
TGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGGAAGTGACACGGACC
GGTCGCCTATCCTGACCACAGCAAAGCGGCCCGGAGCCCGCGGAGGGGACCTGACGGGGG
CGTAGGCGCCGGAAGGCTGGGGGCCCCGGAGCCGGGCCGGCGCGGCCCGAGTTCCGGTGA
GCGGACGGCGGCGCGCGCAGATTTGATAATGGGCTGCATTAAAAGTAAAGAAAACAAAAG
TCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCA
TTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGC
AGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTT
TGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGG
TGGTGTTACTATATTTGTGGCCTTATATGATTATGAAGCTAGAACTACAGAAGACCTTTC
ATTTAAGAAGGGTGAAAGATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGC
AAGATCAATCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCAGA
TTCCATTCANGCAGAAGAATGGTATTTTGGCANAATGGGGAGAAAAGATGCTGAAAGATT
ACTTTTGAATCNCTGGAATCAACGAGGTATTTTCTTTAGTAGAGAGAGTGAAACAACTAA
AGGTGCTTATTCCTTTTCTATTCGTGATTGGGATGAGATAAGGNNGTGACATGTGAAACA
CTACAAATTANGAAACTTGACATGGGTGATACTATATCACANCCAGAGCACATTTGATAC
TCTGCAGNAATGGGTGAACACTAC
>OriGene 3' read for NM_005433 unedited
NTTTTAGCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCA
TTCAATGAGAACTTTTTATTTCAATTATCCACAAAACAATATTACAATACTTTATAAAAA
TATTAAGTTTAGGCTACCATTATTCATTTAAAAAAGTGTGCTAGAAGGCTGTTTTTGCCA
ACTTCCTTTTTTGGTAAGGGTTAACTTCCACATTAAGACACTGAAGACGAAAAGCTGTTG
GAAAATATCTCCAAATTTACAAAGTTGTTTTTCTTGGCAATTTAAAAATACAGAACAATT
TAAAATGAATACACATTGTTAGTGTTTTATCCCTACTATACAATTGTTATTATATAAGGA
ACTGCTCCATTCAGTTAAAACCTAATGAATACCATCAACTTTTCCTGCCTTACTTTCCTG
ATTAGATTTAGTACATAAACGTAATTTTGTCACCCTTTTGAATATGCTTAGAAAAATTAC
AATATCATAGTTCACATAAAGCCCTTAAAAAATCTCATGTCACAAGTTGTTGATAAGAGG
AAATAATGACAGCACTCTTCTTAGGAAGACCCTCTTGGTAGAGAGTGTCAATACTAAGCA
GGTTACGTAAACAGAAAAAACAACCACAATTTTTAGTACCAGAACCACTGCCAACTTGCA
TGAAGTCCATCTTCACATTTTAAAAATGGTTTGAATTTGAACATATACGCCATGGCACAG
AATTTCGAATCTGATGATTAAAATCAGGTAAATAATGATAGCTCAGAATTATATCCTGAA
AATAGAGGGGCCTAATATAAAGGCTTATTTAAAGTGTGACTATATGAGAGTATTTTAAAA
CTATACACCAAACCTTCATTTATTCATCATTTTTCCTTTGCTAGACACTCCAACACCATG
CTGCTATTAAN
Restriction Sites NotI-NotI     
ACCN NM_005433
Insert Size 4230 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005433.3, NP_005424.1
RefSeq Size 4685 bp
RefSeq ORF 1632 bp
Locus ID 7525
UniProt ID P07947
Cytogenetics 18p11.32
Domains pkinase, SH2, TyrKc, SH3, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Adherens junction, Tight junction
Gene Summary This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

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