YES1 (NM_005433) Human Untagged Clone

CAT#: SC116734

YES1 (untagged)-Human v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YES1
• Synonyms: c-yes; HsT441; P61-YES; Yes
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116734 sequence for NM_005433 edited (data generated by NextGen Sequencing)
  ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAAT
  ACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCA
  CCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACA
  CCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTG
  GTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
  GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATA
  ATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT
  TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTT
  GGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGT
  ATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
  TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGT
  GGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTAC
  ACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCT
  CAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG
  GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
  AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAA
  GCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCT
  GAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTT
  AAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATT
  GCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
  AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA
  ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCT
  CCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATT
  CTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAA
  GTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
  CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAA
  TATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGA
  GAAAATTTATAA
  
  Clone variation with respect to NM_005433.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005433 unedited
  TGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGGAAGTGACACGGACC
  GGTCGCCTATCCTGACCACAGCAAAGCGGCCCGGAGCCCGCGGAGGGGACCTGACGGGGG
  CGTAGGCGCCGGAAGGCTGGGGGCCCCGGAGCCGGGCCGGCGCGGCCCGAGTTCCGGTGA
  GCGGACGGCGGCGCGCGCAGATTTGATAATGGGCTGCATTAAAAGTAAAGAAAACAAAAG
  TCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCA
  TTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGC
  AGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTT
  TGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGG
  TGGTGTTACTATATTTGTGGCCTTATATGATTATGAAGCTAGAACTACAGAAGACCTTTC
  ATTTAAGAAGGGTGAAAGATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGC
  AAGATCAATCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCAGA
  TTCCATTCANGCAGAAGAATGGTATTTTGGCANAATGGGGAGAAAAGATGCTGAAAGATT
  ACTTTTGAATCNCTGGAATCAACGAGGTATTTTCTTTAGTAGAGAGAGTGAAACAACTAA
  AGGTGCTTATTCCTTTTCTATTCGTGATTGGGATGAGATAAGGNNGTGACATGTGAAACA
  CTACAAATTANGAAACTTGACATGGGTGATACTATATCACANCCAGAGCACATTTGATAC
  TCTGCAGNAATGGGTGAACACTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005433 unedited
  NTTTTAGCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCA
  TTCAATGAGAACTTTTTATTTCAATTATCCACAAAACAATATTACAATACTTTATAAAAA
  TATTAAGTTTAGGCTACCATTATTCATTTAAAAAAGTGTGCTAGAAGGCTGTTTTTGCCA
  ACTTCCTTTTTTGGTAAGGGTTAACTTCCACATTAAGACACTGAAGACGAAAAGCTGTTG
  GAAAATATCTCCAAATTTACAAAGTTGTTTTTCTTGGCAATTTAAAAATACAGAACAATT
  TAAAATGAATACACATTGTTAGTGTTTTATCCCTACTATACAATTGTTATTATATAAGGA
  ACTGCTCCATTCAGTTAAAACCTAATGAATACCATCAACTTTTCCTGCCTTACTTTCCTG
  ATTAGATTTAGTACATAAACGTAATTTTGTCACCCTTTTGAATATGCTTAGAAAAATTAC
  AATATCATAGTTCACATAAAGCCCTTAAAAAATCTCATGTCACAAGTTGTTGATAAGAGG
  AAATAATGACAGCACTCTTCTTAGGAAGACCCTCTTGGTAGAGAGTGTCAATACTAAGCA
  GGTTACGTAAACAGAAAAAACAACCACAATTTTTAGTACCAGAACCACTGCCAACTTGCA
  TGAAGTCCATCTTCACATTTTAAAAATGGTTTGAATTTGAACATATACGCCATGGCACAG
  AATTTCGAATCTGATGATTAAAATCAGGTAAATAATGATAGCTCAGAATTATATCCTGAA
  AATAGAGGGGCCTAATATAAAGGCTTATTTAAAGTGTGACTATATGAGAGTATTTTAAAA
  CTATACACCAAACCTTCATTTATTCATCATTTTTCCTTTGCTAGACACTCCAACACCATG
  CTGCTATTAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005433
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005433.3, NP_005424.1
• RefSeq Size: 4685 bp
• RefSeq ORF: 1632 bp
• Locus ID: 7525
• UniProt ID: P07947
• Cytogenetics: 18p11.32
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction
• Gene Summary: This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]