LNK (SH2B3) (NM_005475) Human Untagged Clone

SKU SC116715

SH2B3 (untagged)-Human SH2B adaptor protein 3 (SH2B3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LNK
• Synonyms: IDDM20; LNK
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116715 sequence for NM_005475 edited (data generated by NextGen Sequencing); ATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTCTTCCGCGCCCTCAGCCTCCCCGGCG; GCGGCCCCGCGGGGCTGGAGCGAGTTCTGTGAGTTGCACGCCGTAGCGGCGGCCCGGGAG; CTGGCCCGCCAGTACTGGCTGTTCGCCCGGGAGCATCCGCAGCACGCGCCGCTGCGCGCC; GAGCTGGTGTCGCTGCAGTTCACCGACCTCTTCCAGCGCTACTTCTGCCGCGAGGTGCGC; GACGGACGGGCGCCGGGCCGCGACTACCGGGACACAGGCCGTGGGCCCCCAGCCAAGGCC; GAGGCGTCCCCGGAGCCAGGCCCCGGCCCCGCCGCCCCTGGCCTGCCCAAGGCCCGCAGC; TCTGAGGAGCTGGCCCCGCCGCGGCCGCCCGGGCCCTGCTCCTTCCAGCACTTTCGCCGC; AGCCTCCGCCACATCTTCCGCCGCCGCTCGGCCGGGGAGCTGCCAGCGGCCCACACCGCT; GCCGCCCCCGGGACCCCCGGAGAGGCTGCTGAGACCCCCGCCCGGCCTGGCCTGGCCAAG; AAGTTCCTGCCCTGGAGCCTGGCCCGGGAGCCGCCACCCGAGGCGCTGAAGGAGGCGGTG; CTGCGCTACAGCCTGGCCGACGAGGCCTCCATGGACAGCGGGGCACGCTGGCAGCGCGGG; AGGCTGGCGCTGCGCCGGGCCCCGGGCCCCGATGGCCCCGACCGCGTGCTGGAGCTCTTC; GACCCACCCAAGAGTTCAAGGCCCAAGCTACAAGCAGCTTGCTCCAGCATCCAGGAGGTC; CGGCGGTGCACACGGCTTGAGATGCCTGACAACCTTTACACCTTTGTGCTGAAGGTGAAG; GACCGGACAGACATCATCTTTGAGGTGGGAGACGAGCAGCAGCTGAATTCATGGATGGCT; GAGCTCTCGGAGTGCACAGGCCGAGGGCTGGAGAGCACAGAAGCAGAGATGCATATTCCC; TCAGCCCTAGAGCCTAGCACGTCCAGCTCCCCAAGGGGCAGCACAGATTCCCTTAACCAA; GGTGCTTCTCCTGGGGGGCTGCTGGACCCGGCCTGCCAGAAGACGGACCATTTCCTGTCC; TGCTACCCCTGGTTCCACGGCCCCATCTCCAGAGTGAAAGCAGCTCAGCTGGTTCAGCTG; CAGGGCCCTGATGCTCATGGAGTGTTCCTGGTGCGGCAGAGCGAGACGCGGCGTGGGGAA; TACGTGCTCACTTTCAACTTTCAGGGGATAGCCAAGCACCTGCGCCTGTCGCTGACAGAG; CGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCCCTCGGTCGTGGACATGCTCCACCAC; TTCCAGCGCTCGCCCATCCCACTCGAGTGCGGCGCCGCCTGTGATGTCCGGCTCTCCAGC; TACGTGGTAGTCGTCTCCCAACCACCAGGTTCCTGCAACACGGTCCTCTTCCCTTTCTCC; CTTCCTCACTGGGATTCAGAGTCCCTTCCTCACTGGGGTTCAGAGTTGGGCCTTCCCCAC; CTTAGTTCTTCTGGCTGTCCCCGGGGGCTCAGCCCAGAGGGTCTCCCAGGGCGATCCTCA; CCCCCCGAGCAGATCTTCCACCTGGTGCCTTCGCCCGAAGAACTGGCCAACAGCCTGCAG; CACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGACTCGGACTACGAAATGGACTCATCC; TCCCGGAGCCACCTGCGGGCCATAGACAATCAGTACACACCTCTCTGA; ; Clone variation with respect to NM_005475.2; 784 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005475 unedited; TTTCGCCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCA; GAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGA; ATTCGGCACGAGGCGGGCCACCGCCTCCGCCCGGCTGCCCGCCCGGACTGTCGCGGCCCG; CGGTGGCGACGGCGGCCGCTGCAAAGTTTCCCCGGCGGCGGCGGCCCGGGGGCGCATCCT; CCCGCAACTGTCAAGCGCTGGCGGCGGAAATGATGAGGCGCTGGCCATTTTCCGAGCCCG; GGTTTCCTGCCTGAGCCCCGCTCGAGCGAGCCGCGAGCGAGGAGCCGGCGGGCGGGAGAG; GACGCGCCCAGGGCGGGGGCCCGCCCGCCCCCTCGGGATTTCGAGGGCCCGGGGGCGCGC; GACGCCATGGGCCGGCCGGGCCCAGAGCTCCTGTCTCTCAGCCCGGCCGCACCACCTGGG; TCTCCGCCATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTCTTCCGCGCCCTCAGCCT; CCCCGGCGGCGGCCCCGCGGGGCTGGAGCGAGTTCTGTGAGTTGCACGCCGTANCGGCGG; CCCGGGAGCTGGCCCGCCAGTACTGGCTGTTCGCCCGGGAGCATNCGCAGCACGCGCCGC; TGCGCGCCGAGCTGGTGTCGCTGCAGNTCACCGACCTCTTNCAGCGCTACTTCTGCCCGC; GAGGTGCGCGACGGACGGGCGCCGGGCCGCGACTACCGGAACAGGGGCCGTGGGCCCCCA; GCCAGCNCCGAGCGTCCCCGGAGCCAAGCCCCGGCCCGCGCCCTGTCTTGCCAGGCGATN; TTTTGGANNATTGNCCCCCCCGGGCCCCCGGCCCTGGTCCTCCACATTTGGCGCAACTCG; GCATTTCGCCGCTGNGGGGGGGGGGAATGTCCGCGGCCCACCGTGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005475 unedited; CGGTAATACTATGNNACCGCGGCCGCATTCTAGNGATCAGTTTTTTTTTTTTTTTTTTGA; AAACTTTTTAATTCAATATAGCAATTTGTACATTATAGCAAAATTTGCATTATTCAAGAA; TAAGTTACTTGTACAGTACATAAAACAATACATAAAAATTTGCCAAATACCTTCTGCCTA; TAATGATACAAGATGAATCCACTTTATGTTATCACAATGTGCTGTATATTCTAACCAAAC; ACAGGATGTCAGATGTGTCCTTGTTAATATACTCGCAAGTTCCTCTAGCTTGTGGGAGAT; GTTAGAGCTAACACATTTGCAGTAAGGGACTTAGTCCTGAATAGAAAGCATGAAGGAATC; TCAGGCAACCCTCAGGGAAGAGTCCAAGGCCTTGACTTTAGGTTAAGAAACTGTTATGTA; AAAATAGTGTTCTCTGGCCCAAGATTTTAATGATTGCTATTCCTTTTTCCTACGGTCCAG; AAATGATCAAAGGCAGAAGATTTATACCAGATAAAGCCATATGGATTGCTGGTCTAAAAT; TCAAGGCAGGTTAGTTGACTTAATTCTTTGGTGCTGGTGACTGTTAGTTTGTAAAAGTTC; AATAAGTCAAATGAANGAAGGGATGGTGCCGGGAGCTGTCAAGCTGTACTGGTGGGGTCT; GTAATTAGAGCTAACTGGAGGGATCATGATGTCTACTGTCCAGTTTGGTGTTGAGCCATG; GCTCTCGGTAAAATTGCCCGCTGGGGCCCGTTCACGACTGGAAAGAAAATGGTGGGAGTG; CTTTGCCCTTGGGGGGCAAACTGCACCCATGGGGTGCCTGGCCCCACTGGTGCCCACCCC; TTCTAAACTGAAATTGTTCCCCATTAAGAAAAGGGTGGGCTAAGGGCACTTTTTCCTAAA; CCATGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005475
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005475.1, NP_005466.1
• RefSeq Size: 5423 bp
• RefSeq ORF: 1728 bp
• Locus ID: 10019
• UniProt ID: Q9UQQ2
• Cytogenetics: 12q24.12
• Domains: PH, SH2
• Protein Pathways: Neurotrophin signaling pathway
• Summary: This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).