Lysyl tRNA synthetase (KARS) (NM_005548) Human Untagged Clone

SKU SC116645

KARS (untagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lysyl tRNA synthetase
• Synonyms: CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116645 sequence for NM_005548 edited (data generated by NextGen Sequencing); ATGGCGGCCGTGCAGGCGGCCGAGGTGAAAGTGGATGGCAGCGAGCCGAAACTGAGCAAG; AATGAGCTGAAGAGACGCCTGAAAGCTGAGAAGAAAGTAGCAGAGAAGGAGGCCAAACAG; AAAGAGCTCAGTGAGAAACAGCTAAGCCAAGCCACTGCTGCTGCCACCAACCACACCACT; GATAATGGTGTGGGTCCTGAGGAAGAGAGCGTGGACCCAAATCAATACTACAAAATCCGC; AGTCAAGCAATTCATCAGCTGAAGGTCAATGGGGAAGACCCATACCCACACAAGTTCCAT; GTAGACATCTCACTCACTGACTTCATCCAAAAATATAGTCACCTGCAGCCTGGGGATCAC; CTGACTGACATCACCTTAAAGGTGGCAGGTAGGATCCATGCCAAAAGAGCTTCTGGGGGA; AAGCTCATCTTCTATGATCTTCGAGGAGAGGGGGTGAAGTTGCAAGTCATGGCCAATTCC; AGAAATTATAAATCAGAAGAAGAATTTATTCATATTAATAACAAACTGCGTCGGGGAGAC; ATAATTGGAGTTCAGGGGAATCCTGGTAAAACCAAGAAGGGTGAGCTGAGCATCATTCCG; TATGAGATCACACTGCTGTCTCCCTGTTTGCATATGTTACCTCATCTTCACTTTGGCCTC; AAAGACAAGGAAACAAGGTATCGCCAGAGATACTTGGACTTGATCCTGAATGACTTTGTG; AGGCAGAAATTTATCATCCGCTCTAAGATCATCACATATATAAGAAGTTTCTTAGATGAG; CTGGGATTCCTAGAGATTGAAACTCCCATGATGAACATCATCCCAGGGGGAGCCGTGGCC; AAGCCTTTCATCACTTATCACAACGAGCTGGACATGAACTTATATATGAGAATTGCTCCA; GAACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTTTATGAAATTGGACGC; CAGTTCCGGAATGAGGGGATTGATTTGACGCACAATCCTGAGTTCACCACCTGTGAGTTC; TACATGGCCTATGCAGACTATCACGATCTCATGGAAATCACGGAGAAGATGGTTTCAGGG; ATGGTGAAGCATATTACAGGCAGTTACAAGGTCACCTACCACCCAGATGGCCCAGAGGGC; CAAGCCTACGATGTTGACTTCACCCCACCCTTCCGGCGAATCAACATGGTAGAAGAGCTT; GAGAAAGCCCTGGGGATGAAGCTGCCAGAAACGAACCTCTTTGAAACTGAAGAAACTCGC; AAAATTCTTGATGATATCTGTGTGGCAAAAGCTGTTGAATGCCCTCCACCTCGGACCACA; GCCAGGCTCCTTGACAAGCTTGTTGGGGAGTTCCTGGAAGTGACTTGCATCAATCCTACA; TTCATCTGTGATCACCCACAGATAATGAGCCCTTTGGCTAAATGGCACCGCTCTAAAGAG; GGTCTGACTGAGCGCTTTGAGCTGTTTGTCATGAAGAAAGAGATATGCAATGCGTATACT; GAGCTGAATGATCCCATGCGGCAGCGGCAGCTTTTTGAAGAACAGGCCAAGGCCAAGGCT; GCAGGTGATGATGAGGCCATGTTCATAGATGAAAACTTCTGTACTGCCCTGGAATATGGG; CTGCCCCCCACAGCTGGCTGGGGCATGGGCATTGATCGAGTCGCCATGTTTCTCACGGAC; TCCAACAACATCAAGGAAGTACTTCTGTTTCCTGCCATGAAACCCGAAGACAAGAAGGAG; AATGTAGCAACCACTGATACACTGGAAAGCACAACAGTTGGCACTTCTGTCTAG; ; Clone variation with respect to NM_005548.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005548 unedited; TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGTACTATCC; TCCTTACTTTTGGGTCGGGCCCTCCGGGAAGAGGCGGCCGTGCAGGCGGCCGAGGTGAAA; GTGGATGGCAGCGAGCCGAAACTGAGCAAGAATGAGCTGAAGAGACGCCTGAAAGCTGAG; AAGAAAGTAGCAGAGAAGGAGGCCAAACAGAAAGAGCTCAGTGAGAAACAGCTAAGCCAA; GCCACTGCTGCTGCCACCAACCACACCACTGATAATGGTGTGGGTCCTGAGGAAGAGAGC; GTGGACCCAAATCAATACTACAAAATCCGCAGTCAAGCAATTCATCAGCTGAAGGTCAAT; GGGGAAGACCCATACCCACACAAGTTCCATGTAGACATCTCACTCACTGACTTCATCCAA; AAATATAGTCACCTGCAGCCTGGGGATCACCTGACTGACATCACCTTAAAGGTGGCAGGT; AGGATCCATGCCAAAAGAGCTTCTGGGGGAAAGCTCATCTTCTATGATCTTCGAGGAGAG; GGGGTGAAGTTGCAAGTCATGGCCAATTCCAGAAATTATAAATCAGAAGAAGAATTTATT; CATATTAATAACAAACTGCGTCGGGGAGACATAATTGGAGTTCAGGGGAATCCTGGTAAA; ACCAAGAAGGGTGAGCTGAGCATCATTCCGTATGAGATCACACTGCTGTCNTCCCTGTTG; CATATGTTACCTCATCTTCACTTTGGCCNTCAAGACAGGGAAACANGGTATCGCCAGAGA; TACTTGGACTTGATCCTGAATGACTTTGTGAGGCAGAAATTATCATCCGCTCTAAGACAT; CACATATATAAGAAGTTNCTTAGATGAGCTGGGATTCCCTAGAGATGAACTCCCATGATG; ACATCATCCCAGGGGNACGCCGTGGCCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005548 unedited; AGAGTCGAGTTTTTTTTTTTTTTTTTTGGTAACAGGATTAAAAAGAAATTTTTAATTCCT; TGTCTCTCTTCTGATGGCTGAACAGAACTGCGGTGTCAAATGGAAAGCAGCACACAAGAA; TTCCCTTGCAGACCTTGATCTTTCGCAGAAATGCAAAGACGCCTGAGTTATACAACTTGC; AATTATTATTTTCTAGACAGAAGTGCCAACTGTTGTGCTTTCCAGTGTATCAGTGGTTGC; TACATTCTCCTTCTTGTCTTCGGGTTTCATGGCAGGAAACAGAAGTACTTCCTTGATGTT; GTTGGAGTCCGTGAGAAACATGGCGACTCGATCAATGCCCATGCCCCAGCCAGCTGTGGG; GGGCAGCCCATATTCCAGGGCAGTACAGAAGTTTTCATCTATGAACATGGCCTCATCATC; ACCTGCAGCCTTGGCCTTGGCCTGTTCTTCAAAAAGCTGCCGCTGCCGCATGGGATCATT; CAGCTCAGTATACGCATTGCATATCTCTTTCTTCATGACAAACAGCTCAAAGCGCTCAGT; CAGACCCTCTTTAGAGCCGTGCCATTTAGCCAAAGGGCTCATTATCTGTGGGTGATCACA; GATGAATGTANGATTGATGCAAGTTACTTCCCAGAACTCCCAACAAGCTTGTCAAGAGCC; TGGCTGTGGTCGAGGGGGAGGCATCCACAGCTTTTGCCAACAGATATATCAAAAATTTGC; GAGTTCCTCAGTTCAAGGAGGTNGTTCTTGGAGTTAATTCCAAGGCTTTTTAAGCTTTTA; CATGTGATTCCCGCAGGTGGGGGAATAACATGGAGGCTGGCCTTTGGCCCTCGGTGGAAG; GACCTGAACGCCGGAAAGCTTACCTCCCGAACCTTTTTCGGATTCATGAACGGAATCCGC; AGCCTTAAACCCGGGGGACCGGTTGGGAAAAACCCTTTTGGAAGGGCCTTATAACCGGAG; CCCCCCAACTTTGAAGCTGGGACCTTTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_005548
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005548.1, NP_005539.1
• RefSeq Size: 1997 bp
• RefSeq ORF: 1794 bp
• Locus ID: 3735
• UniProt ID: Q15046
• Cytogenetics: 16q23.1
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.