Lysyl tRNA synthetase (KARS) (NM_005548) Human Untagged Clone

CAT#: SC116645

KARS (untagged)-Human lysyl-tRNA synthetase (KARS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lysyl tRNA synthetase
• Synonyms: CMTRIB; DEAPLE; DFNB89; KARS; KARS2; KRS; LEPID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116645 sequence for NM_005548 edited (data generated by NextGen Sequencing)
  ATGGCGGCCGTGCAGGCGGCCGAGGTGAAAGTGGATGGCAGCGAGCCGAAACTGAGCAAG
  AATGAGCTGAAGAGACGCCTGAAAGCTGAGAAGAAAGTAGCAGAGAAGGAGGCCAAACAG
  AAAGAGCTCAGTGAGAAACAGCTAAGCCAAGCCACTGCTGCTGCCACCAACCACACCACT
  GATAATGGTGTGGGTCCTGAGGAAGAGAGCGTGGACCCAAATCAATACTACAAAATCCGC
  AGTCAAGCAATTCATCAGCTGAAGGTCAATGGGGAAGACCCATACCCACACAAGTTCCAT
  GTAGACATCTCACTCACTGACTTCATCCAAAAATATAGTCACCTGCAGCCTGGGGATCAC
  CTGACTGACATCACCTTAAAGGTGGCAGGTAGGATCCATGCCAAAAGAGCTTCTGGGGGA
  AAGCTCATCTTCTATGATCTTCGAGGAGAGGGGGTGAAGTTGCAAGTCATGGCCAATTCC
  AGAAATTATAAATCAGAAGAAGAATTTATTCATATTAATAACAAACTGCGTCGGGGAGAC
  ATAATTGGAGTTCAGGGGAATCCTGGTAAAACCAAGAAGGGTGAGCTGAGCATCATTCCG
  TATGAGATCACACTGCTGTCTCCCTGTTTGCATATGTTACCTCATCTTCACTTTGGCCTC
  AAAGACAAGGAAACAAGGTATCGCCAGAGATACTTGGACTTGATCCTGAATGACTTTGTG
  AGGCAGAAATTTATCATCCGCTCTAAGATCATCACATATATAAGAAGTTTCTTAGATGAG
  CTGGGATTCCTAGAGATTGAAACTCCCATGATGAACATCATCCCAGGGGGAGCCGTGGCC
  AAGCCTTTCATCACTTATCACAACGAGCTGGACATGAACTTATATATGAGAATTGCTCCA
  GAACTCTATCATAAGATGCTTGTGGTTGGTGGCATCGACCGGGTTTATGAAATTGGACGC
  CAGTTCCGGAATGAGGGGATTGATTTGACGCACAATCCTGAGTTCACCACCTGTGAGTTC
  TACATGGCCTATGCAGACTATCACGATCTCATGGAAATCACGGAGAAGATGGTTTCAGGG
  ATGGTGAAGCATATTACAGGCAGTTACAAGGTCACCTACCACCCAGATGGCCCAGAGGGC
  CAAGCCTACGATGTTGACTTCACCCCACCCTTCCGGCGAATCAACATGGTAGAAGAGCTT
  GAGAAAGCCCTGGGGATGAAGCTGCCAGAAACGAACCTCTTTGAAACTGAAGAAACTCGC
  AAAATTCTTGATGATATCTGTGTGGCAAAAGCTGTTGAATGCCCTCCACCTCGGACCACA
  GCCAGGCTCCTTGACAAGCTTGTTGGGGAGTTCCTGGAAGTGACTTGCATCAATCCTACA
  TTCATCTGTGATCACCCACAGATAATGAGCCCTTTGGCTAAATGGCACCGCTCTAAAGAG
  GGTCTGACTGAGCGCTTTGAGCTGTTTGTCATGAAGAAAGAGATATGCAATGCGTATACT
  GAGCTGAATGATCCCATGCGGCAGCGGCAGCTTTTTGAAGAACAGGCCAAGGCCAAGGCT
  GCAGGTGATGATGAGGCCATGTTCATAGATGAAAACTTCTGTACTGCCCTGGAATATGGG
  CTGCCCCCCACAGCTGGCTGGGGCATGGGCATTGATCGAGTCGCCATGTTTCTCACGGAC
  TCCAACAACATCAAGGAAGTACTTCTGTTTCCTGCCATGAAACCCGAAGACAAGAAGGAG
  AATGTAGCAACCACTGATACACTGGAAAGCACAACAGTTGGCACTTCTGTCTAG
  
  Clone variation with respect to NM_005548.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005548 unedited
  TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGTACTATCC
  TCCTTACTTTTGGGTCGGGCCCTCCGGGAAGAGGCGGCCGTGCAGGCGGCCGAGGTGAAA
  GTGGATGGCAGCGAGCCGAAACTGAGCAAGAATGAGCTGAAGAGACGCCTGAAAGCTGAG
  AAGAAAGTAGCAGAGAAGGAGGCCAAACAGAAAGAGCTCAGTGAGAAACAGCTAAGCCAA
  GCCACTGCTGCTGCCACCAACCACACCACTGATAATGGTGTGGGTCCTGAGGAAGAGAGC
  GTGGACCCAAATCAATACTACAAAATCCGCAGTCAAGCAATTCATCAGCTGAAGGTCAAT
  GGGGAAGACCCATACCCACACAAGTTCCATGTAGACATCTCACTCACTGACTTCATCCAA
  AAATATAGTCACCTGCAGCCTGGGGATCACCTGACTGACATCACCTTAAAGGTGGCAGGT
  AGGATCCATGCCAAAAGAGCTTCTGGGGGAAAGCTCATCTTCTATGATCTTCGAGGAGAG
  GGGGTGAAGTTGCAAGTCATGGCCAATTCCAGAAATTATAAATCAGAAGAAGAATTTATT
  CATATTAATAACAAACTGCGTCGGGGAGACATAATTGGAGTTCAGGGGAATCCTGGTAAA
  ACCAAGAAGGGTGAGCTGAGCATCATTCCGTATGAGATCACACTGCTGTCNTCCCTGTTG
  CATATGTTACCTCATCTTCACTTTGGCCNTCAAGACAGGGAAACANGGTATCGCCAGAGA
  TACTTGGACTTGATCCTGAATGACTTTGTGAGGCAGAAATTATCATCCGCTCTAAGACAT
  CACATATATAAGAAGTTNCTTAGATGAGCTGGGATTCCCTAGAGATGAACTCCCATGATG
  ACATCATCCCAGGGGNACGCCGTGGCCAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005548 unedited
  AGAGTCGAGTTTTTTTTTTTTTTTTTTGGTAACAGGATTAAAAAGAAATTTTTAATTCCT
  TGTCTCTCTTCTGATGGCTGAACAGAACTGCGGTGTCAAATGGAAAGCAGCACACAAGAA
  TTCCCTTGCAGACCTTGATCTTTCGCAGAAATGCAAAGACGCCTGAGTTATACAACTTGC
  AATTATTATTTTCTAGACAGAAGTGCCAACTGTTGTGCTTTCCAGTGTATCAGTGGTTGC
  TACATTCTCCTTCTTGTCTTCGGGTTTCATGGCAGGAAACAGAAGTACTTCCTTGATGTT
  GTTGGAGTCCGTGAGAAACATGGCGACTCGATCAATGCCCATGCCCCAGCCAGCTGTGGG
  GGGCAGCCCATATTCCAGGGCAGTACAGAAGTTTTCATCTATGAACATGGCCTCATCATC
  ACCTGCAGCCTTGGCCTTGGCCTGTTCTTCAAAAAGCTGCCGCTGCCGCATGGGATCATT
  CAGCTCAGTATACGCATTGCATATCTCTTTCTTCATGACAAACAGCTCAAAGCGCTCAGT
  CAGACCCTCTTTAGAGCCGTGCCATTTAGCCAAAGGGCTCATTATCTGTGGGTGATCACA
  GATGAATGTANGATTGATGCAAGTTACTTCCCAGAACTCCCAACAAGCTTGTCAAGAGCC
  TGGCTGTGGTCGAGGGGGAGGCATCCACAGCTTTTGCCAACAGATATATCAAAAATTTGC
  GAGTTCCTCAGTTCAAGGAGGTNGTTCTTGGAGTTAATTCCAAGGCTTTTTAAGCTTTTA
  CATGTGATTCCCGCAGGTGGGGGAATAACATGGAGGCTGGCCTTTGGCCCTCGGTGGAAG
  GACCTGAACGCCGGAAAGCTTACCTCCCGAACCTTTTTCGGATTCATGAACGGAATCCGC
  AGCCTTAAACCCGGGGGACCGGTTGGGAAAAACCCTTTTGGAAGGGCCTTATAACCGGAG
  CCCCCCAACTTTGAAGCTGGGACCTTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005548
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005548.1, NP_005539.1
• RefSeq Size: 1997 bp
• RefSeq ORF: 1794 bp
• Locus ID: 3735
• UniProt ID: Q15046
• Cytogenetics: 16q23.1
• Domains: tRNA-synt_2, tRNA_anti
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.