Arp3 (ACTR3) (NM_005721) Human Untagged Clone

SKU SC116577

ACTR3 (untagged)-Human ARP3 actin-related protein 3 homolog (yeast) (ACTR3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Arp3
• Synonyms: ARP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116577 sequence for NM_005721 edited (data generated by NextGen Sequencing); ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGA; TATGCTGGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCA; GCAAAAGTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTC; TTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCAT; GGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATAT; TTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA; GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTAC; ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAA; CGGACGTTGACCGGTACGGTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTG; GCTGAAGGGTATGTGATTGGCAGCTGTATTAAACACATTCCAATCGCAGGACGAGATATA; ACATATTTTATTCAGCAACTGCTGAGAGACCGAGAAGTAGGAATCCCTCCAGAACAATCC; TTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGCCCAGATTTAGTAAAA; GAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGAATCAAT; GCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAA; ATCTTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTA; GATGAAGTAATTCAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTC; CTCTCTGGAGGTTCAACCATGTTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAA; AGAACTGTAGATGCCCGGCTGAAATTAAGTGAGGAATTGAGTGGTGGTAGATTGAAGCCA; AAACCTATTGATGTACAAGTCATTACACACCACATGCAGCGATATGCAGTTTGGTTTGGA; GGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTAT; GAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCGTAA; ; Clone variation with respect to NM_005721.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005721 unedited; TTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTGCGGAAGTGATAGC; CGCCGACCGAGCCTGCTGCTTTCTTGCTACTGCTTCGGCTTCCCGGCTACCCCCCGGACG; GTGAAGGCGGCCCAGCTGTGGATGGTCAGATAGCCCTTGTCTCCCGCCGCCAATCTCTGG; CCCCTAGCAGCACGGAGCAGACGGCGGCAGCAGCAGCAGCAGGCGAGGAGGAAGATGGCG; GGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGATATGCT; GGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCAGCAAAA; GTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTCTTCATT; GGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCATGGTATA; GTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATATTTAAGG; GCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCAGAAAAC; AGGGAATATACTGCTGAAATAATGTTTGGAGTCCTTCAATGTTCCAGGCTTGTACATTGC; TGTGCANGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAGTANGAGAACGGACG; TTGACCGGTACCGGTATAGACAGTGGAGGATGGTGTCACTCATGTCATTCCTGTGGCTGA; AGGGGATGTGATTGGCAGCTGTATTAAACACATTCCATCGCAGGACGAGATATACATAAT; TTATTCAGCACTGCTGAGAGACCGAAAGTAGGAATTCTCCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005721 unedited; GCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGG; AATTCAGTTTTAATTTATTTTCATCACTTTTTTCTTCATAATCCAGATATTTTAAAATGC; AAAGAAAATTAACTTTCAATGATATGTTCAGGGACTGGCACTAAAAAAATTTTCAAACTG; CAAATGAGTTATACAAATGAAAATATCAAATGGAGATCCAGTTATCAAAATGAAAGCACT; CAACATATTAAAAGTTCACAAGTATTTGTATTGAGCACATTACAAAAGTCAGCTTGCTAA; CTGTTGGGATTTTAAAGAACTATTGCAAAAGCCTGAAAAAAATAAATTTATTAGTTAACT; TATAAAGAGATTAAAGAGGCTGAAACAAGTATTAAAAAGAAATTTGTGCCTTTATTAAAA; TGTTAGGCTTTAAATATGCCAATTTTGATAAATTATTGGTGGTGTTTTGTTTTTTATAAA; AAATGACACTACAGAACTGCAATACTGGTCCAACACTCTTGGCTTTACTCTTCTTTTAAA; GCAAGAAACAGAATGCGAGTAATCAGAAAGCACTAGCAGGCATCAGTTAATCCAAGATAC; TAGCTCTTATTTCCAAAAGCACTTGCAAAGAAAACCTTTTGGGGGGAAGGGGCGGAGAGG; GATGGAACCACTCCATAATAACTGGAATCCCATGAGTGTGTATGCCAAGTCTCATGAGGC; TATTTTTTGAATTTATCCTTTACTTGGACATGGTTTTTTCCCTCAAATACAATTTTTCTT; TGACTTTNTTTCCTCAAGTATAAAAGTATGAAATTAAACAAGCTCTTGACTGCACACTAN; AAGTGTACAATCAGCATATAGAGCTTCTACCACTGATAAACCCATCGATCTNGATAATCA; TTATATACAAATATCGGCACAGAAAGACTAAACCACTTCTTCTGTACCCTAGATCAATAT; CATCTAGAAAACCAACCTTCCTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_005721
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005721.3, NP_005712.1
• RefSeq Size: 2728 bp
• RefSeq ORF: 1257 bp
• Locus ID: 10096
• UniProt ID: P61158
• Cytogenetics: 2q14.1
• Domains: ACTIN
• Protein Families: Druggable Genome
• Summary: The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.