CERT1 (NM_005713) Human Untagged Clone

CAT#: SC116574

COL4A3BP (untagged)-Human collagen, type IV, alpha 3 (Goodpasture antigen) binding protein (COL4A3BP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CERT1
• Synonyms: CERT; CERTL; COL4A3BP; GPBP; MRD34; STARD11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005713, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGATAATCAGAGCTGGAACTCGTCGGGCTCGGAGGAGGATCCAGAGACGGAGTCTGGGCCGCCTG
  TGGAGCGCTGCGGGGTCCTCAGTAAGTGGACAAACTACATTCATGGGTGGCAGGATCGTTGGGTAGTTTT
  GAAAAATAATGCTCTGAGTTACTACAAATCTGAAGATGAAACAGAGTATGGCTGCAGAGGATCCATCTGT
  CTTAGCAAGGCTGTCATCACACCTCACGATTTTGATGAATGTCGATTTGATATTAGTGTAAATGATAGTG
  TTTGGTATCTTCGTGCTCAGGATCCAGATCATAGACAGCAATGGATAGATGCCATTGAACAGCACAAGAC
  TGAATCTGGATATGGATCTGAATCCAGCTTGCGTCGACATGGCTCAATGGTGTCCCTGGTGTCTGGAGCA
  AGTGGCTACTCTGCAACATCCACCTCTTCATTCAAGAAAGGCCACAGTTTACGTGAGAAGTTGGCTGAAA
  TGGAAACATTTAGAGACATCTTATGTAGACAAGTTGACACGCTACAGAAGTACTTTGATGCCTGTGCTGA
  TGCTGTCTCTAAGGATGAACTTCAAAGGGATAAAGTGGTAGAAGATGATGAAGATGACTTTCCTACAACG
  CGTTCTGATGGTGACTTCTTGCATAGTACCAACGGCAATAAAGAAAAGTTATTTCCACATGTGACACCAA
  AAGGAATTAATGGTATAGACTTTAAAGGGGAAGCGATAACTTTTAAAGCAACTACTGCTGGAATCCTTGC
  AACACTTTCTCATTGTATTGAACTAATGGTTAAACGTGAGGACAGCTGGCAGAAGAGACTGGATAAGGAA
  ACTGAGAAGAAAAGAAGAACAGAGGAAGCATATAAAAATGCAATGACAGAACTTAAGAAAAAATCCCACT
  TTGGAGGACCAGATTATGAAGAAGGCCCTAACAGTCTGATTAATGAAGAAGAGTTCTTTGATGCTGTTGA
  AGCTGCTCTTGACAGACAAGATAAAATAGAAGAACAGTCACAGAGTGAAAAGGTGAGATTACATTGGCCT
  ACATCCTTGCCCTCTGGAGATGCCTTTTCTTCTGTGGGGACACATAGATTTGTCCAAAAGCCCTATAGTC
  GCTCTTCCTCCATGTCTTCCATTGATCTAGTCAGTGCCTCTGATGATGTTCACAGATTCAGCTCCCAGGT
  TGAAGAGATGGTGCAGAACCACATGACTTACTCATTACAGGATGTAGGCGGAGATGCCAATTGGCAGTTG
  GTTGTAGAAGAAGGAGAAATGAAGGTATACAGAAGAGAAGTAGAAGAAAATGGGATTGTTCTGGATCCTT
  TAAAAGCTACCCATGCAGTTAAAGGCGTCACAGGACATGAAGTCTGCAATTATTTCTGGAATGTTGACGT
  TCGCAATGACTGGGAAACAACTATAGAAAACTTTCATGTGGTGGAAACATTAGCTGATAATGCAATCATC
  ATTTATCAAACACACAAGAGGGTGTGGCCTGCTTCTCAGCGAGACGTATTATATCTTTCTGTCATTCGAA
  AGATACCAGCCTTGACTGAAAATGACCCTGAAACTTGGATAGTTTGTAATTTTTCTGTGGATCATGACAG
  TGCTCCTCTAAACAACCGATGTGTCCGTGCCAAAATAAATGTTGCTATGATTTGTCAAACCTTGGTAAGC
  CCACCAGAGGGAAACCAGGAAATTAGCAGGGACAACATTCTATGCAAGATTACATATGTAGCTAATGTGA
  ACCCTGGAGGATGGGCACCAGCCTCAGTGTTAAGGGCAGTGGCAAAGCGAGAGTATCCTAAATTTCTAAA
  ACGTTTTACTTCTTACGTCCAAGAAAAAACTGCAGGAAAGCCTATTTTGTTCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005713 unedited
  GGCGGGGGGGNNCNNTTTTTCCCCCCCNNGGTTCAAAATTGTATACGACTCCTATAGGCG
  GCCGCGNAATTCGCACGAGGCGGTAGCGGAGGTGTGAGTGGACGCGGGACTCAGCGGCCG
  GATTTTCTCTTCCCTTCTTTTCCCTTTTCCTTCCCTATTTGAAATTGGCATCGAGGGGGC
  TAAGTTCGGGTGGCAGCGCCGGGCGCAACGCAGGGGTCACGGCGACGGCGGCGGCGGCTG
  ACGGCTGGAAGGGTAGGCTTCCTTCACCGCTCGTCCTCCTTCCTCGCTCCGCTCGGTGTC
  AGGCGCGGCGGCGGCGCGGCGGGCGGACTTCGTCCCTCCTCCTGCTCCCCCCCACACCGG
  AGCGGGCACTCTTCGCTTCGCCATCCCCCGACCCTTCACCCCGAGGACTGGGCGCCTCCT
  CCGGCGCAGCTGAGGGAGCGGGGGCCGGTCTCCTGCTCGGTTGTCGAGCCTCCATGTCGG
  ATAATCAGAGCTGGAACTCGTCGGGCTCGGAGGAGGATCCAGAGACGGAGTCTGGGCCGC
  CTGTGGAGCGCTGCGGNGTCCTCAGTAAGTGGACAAACTACATTCATGGGTGGCAGGATC
  GTTGGGTAGTTTTGAAAAATAATGCTCTGAGTTACTACAAATCTGAAGATGAAACAGAGT
  ATGGCTGCAGAGGATCCATCTGTCTTAGCAAGGCTGTCATCACACCTCACGATTTTGATG
  AATGTCGATTTGATATTAGTGTAAATGATAGTGTTTGGTATCCTTCGTGCTCAGGATCCA
  GATCATAGACAGCAATGGATAGATGCCATTTGACAGCACAAGACTGGATCTGGATATGGA
  TCTGAATCCACCTTGCGTCGACATGGCTAAATGTGGCCCCTGGTGTCTGAAGCAGTGGTT
  ACTTCTGCACATCCACCTCTTCATTCAGGAAAGCCACANTTACATGAAAAGTTGGCTAAA
  TGGAACATTTAAGACATCTAGGTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005713 unedited
  CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTCCAACATGGAATGTCA
  ACAGCCTTGCTTCAGTACTGTTAATACTACAACAAAATAGGCTTTCCTGCAGTTTTTTCT
  TGGACGTAAGAAGCAAAACGTTTTAAAAATTTAGGATACTCTCGCTTTGCCACTGCCCTT
  TAACACTGAGGCTGGTGCCCATTCCTCAGGGGTCACATTACCTCCATGGGGAATCTTGCC
  TAGAATGTTGTCCCCTGCTAATTGCCTGGCTCCCCTATGGAGGGCTTACCAAGGTTTGCA
  AAATAATAGCAACCTTTTATCTTCCGCCCGCACACATCGGCTGTTAAGAGGAGAACTTGG
  GTTGACCAACAGACAACTCCCACCTCTCCCCCTTCTACGGCCATTTTTCACCCCGGGCTG
  TGTTTTTCTAATTGACAGAAATATACTTTTCCTTTTTCCTGAGTATAAGTCCTCCCCCTC
  CTTTTGTGTTTTTATTATTATTACCGCCTTCTCTTCGTGTCTCTGAGCCCGCGCTCACGC
  CATGTTCGTGTGCTGTCAGACGCGCGACGGCATCCGACCCCGCGCGGACGCCACCCTCCC
  CCCCCGCCCTTCCGTCCTCCCACCCCCCACGCCCACCCCCCCCCGGAGGCCCACTCCTTT
  CCTTCTGGCCGTACTCCTCTTGGGCGTGTCGTGCTCCGCTTGGCGCAGCGCCCGCCTCAG
  ACGACCACACGGAACCGCTCAACGCACTTCGCTGCGCACCGATTGTCCGTAGTCCATCCG
  CTGTATGGTACCGCCCATCGACCACCACTAAGGCCCGCTCTCGCTCTCTCTCTTTCCTCA
  CCTTCTCCCCTTCTGACCGCCCCTGCCATCTCACTACATCCGCCCCCTCCTCCCTCCAAA
  CCCTCGCCGCTACCTCCCCCCTCCTCCACCCGCCTTCCGCCCCCCGCAGCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005713
• Insert Size: 2290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005713.1, NP_005704.1
• RefSeq Size: 5395 bp
• RefSeq ORF: 1875 bp
• Locus ID: 10087
• UniProt ID: Q9Y5P4
• Cytogenetics: 5q13.3
• Domains: PH, START
• Gene Summary: This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding region compared to variant 3. The resulting isoform (1) is shorter at the N-terminus compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.