G3BP (G3BP1) (NM_005754) Human Untagged Clone

CAT#: SC116551

G3BP1 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 1 (G3BP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: G3BP
• Synonyms: G3BP; HDH-VIII
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116551 sequence for NM_005754 edited (data generated by NextGen Sequencing)
  ATGGTGATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAATTTGTGAGACAGTATTAC
  ACACTGCTGAACCAGGCCCCAGACATGCTGCATAGATTTTANNNNNNNNNCTCTTCTTAT
  GTCCATGGGGGATTGGATTCAAATGGAAAGCCAGCAGATGCAGTCTACGGACAGAAAGAA
  ATCCACAGGAAAGTGATGTCACAAAACTTCACCAACTGCCACACCAAGATTCGCCATGTT
  GATGCTCATGCCACGCTAAATGATGGTGTGGTAGTCCAGGTGATGGGGCTTCTCTCTAAC
  AACAACCAGGCTTTGAGGAGATTCATGCAAACGTTTGTCCTTGCTCCTGAGGGGTCTGTT
  GCAAATAAATTCTATGTTCACAATGATATCTTCAGATACCAAGATGAGGTCTTTGGTGGG
  TTTGTCACTGAGCCTCAGGAGGAGTCTGAAGAAGAAGTAGAGGAACCTGAAGAAAGACAG
  CAAACACCTGAGGTGGTACCTGATGATTCTGGAACTTTCTATGATCAGGCAGTTGTCAGT
  AATGACATGGAAGAACATTTAGAGGAGCCTGTTGCTGAACCAGAGCCTGATCCTGAACCA
  GAACCAGAACAAGAACCTGTATCTGAAATCCAAGAGGAAAAGCCTGAGCCAGTATTAGAA
  GAAACTGCCCCTGAGGATGCTCAGAAGAGTTCTTCTCCAGCACCTGCAGACATAGCTCAG
  ACAGTACAGGAAGACTTGAGGACATTTTCTTGGGCATCTGTGACCAGTAAGAATCTTCCA
  CCCAGTGGAGCTGTTCCAGTTACTGGGATACCACCTCATGTTGTTAAAGTACCAGCTTCA
  CAGCCCCGTCCAGAGTCTAAGCCTGAATCTCAGATTCCACCACAAAGACCTCAGCGGGAT
  CAAAGAGTGCGAGAACAACGAATAAATATTCCTCCCCAAAGGGGACCCAGACCAATCCGT
  GAGGCTGGTGAGCAAGGTGACATTGAACCCCGAAGAATGGTGAGACACCCTGACAGTCAC
  CAACTCTTCATTGGCAACCTGCCTCATGAAGTGGACAAATCAGAGCTTAAAGATTTCTTT
  CAAAGTTATGGAAACGTGGTGGAGTTGCGCATTAACAGTGGTGGGAAATTACCCAATTTT
  GGTTTTGTTGTGTTTGATGATTCTGAGCCTGTTCAGAAAGTCCTTAGCAACAGGCCCATC
  ATGTTCAGAGGTGAGGTCCGTCTGAATGTCGAAGAGAAGAAGACTCGAGCTGCCAGGGAA
  GGCGACCGACGAGATAATCGCCTTCGGGGACCTGGAGGCCCTCGAGGTGGGCTGGGTGGT
  GGAATGAGAGGCCCTCCCCGTGGAGGCATGGTGCAGAAACCAGGATTTGGAGTGGGAAGG
  GGGCTTGCGCCACGGCAGTGA
  
  Clone variation with respect to NM_005754.2
  102 t=>n;103 g=>n;104 g=>n;105 a=>n;106 a=>n;107 a=>n;108 g=>n;109 a=>n;110 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005754 unedited
  GTGCAAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGTGCGTG
  AGGGGTTTGTACTATCCTCGGTGCTGTGGTGCAGAGCTAGTTCCTCTCCAGCTCAGCCGC
  GTAGGTTTGGACATATTTGACTCTTTTCCCCCCAGGTTGAATTGACCAAAGCAATGGTGA
  TGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAATTTGTGAGACAGTATTACACACTGC
  TGAACCAGGCCCCAGACATGCTGCATAGATTTTATGGAAAGAACTCTTCTTATGTCCATG
  GGGGATTGGATTCAAATGGAAAGCCAGCAGATGCAGTCTACGGACAGAAAGAAATCCACA
  GGAAAGTGATGTCACAAAACTTCACCAACTGCCACACCAAGATTCGCCATGTTGATGCTC
  ATGCCACGCTAAATGATGGTGTGGTAGTCCAGGTGATGGGGCTTCTCTCTAACAACAACC
  AGGCTTTGAGGAGATTCATGCAAACGTTTGTCCTTGCTCCTGAGGGGTCTGTTGCAAATA
  AATTCTATGTTCACAATGATATCTTCAGATACCAAGATGAGGTCTTTGGTGGGTTTGTCA
  CTGAGCCTCAGGAGGAGTCTGAAGAAGAAGTAGAGGAACCTGAAGAAAGACAGCAAACAC
  CTGAGGTGGTACCTGATGATTCTGGAACTTTCTATGATCANGCAGTTGTCAGTAATGACA
  TGGAAGAACATTTAGAGGAGCCTGTTGCTGAACCAGAGCCTGATTCTTGACCAGAACCAG
  ACCAGAACCTGTATCTGAAATCCCAGAGGAAAGCCTGAGCCATATTAGAAGAACTGCCCC
  TGTAGATGCTCAGAAGAGTTCTTCTCCAGCACTGCCGACTAGCTCAGCAGTACACGAAGA
  CTTGAGACATTTTTTTGGCATCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005754 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTCCAGAATATTTTAATAAAAATT
  GGCCATTTCCAAGTAACACCCACTGCATGCCCATCAATAAAAGAGAACCATAACAAAATA
  GAAAATTTACCATATGGCTATGATTTTAAGAAAAAAAAAAATGCGGACACGAGCACATGA
  GCAAAGTCCAATATTAAAGTAACATTCCTTCTTACGGGCTATGATTAGGAATCTGATTAG
  ATACCAGAATAAAAATACAAATGCTGAACTATTTAGGTCAAAGAACCTCTATAGAAGCAT
  CTGTCTTCAAAGGCATTCCAGTTTGAGACTCAATTTTAGAATAAGGCATAACAAATATGT
  TAACAAAAGACATACACAAAGTGATGAAAGAGGGATTCTATAATTCCCCACATACTCTGA
  ACCTAATAAAACAAATCTAGATTTTTTTTTTAAACAATTGCCAAGACTGAAATCAAAGAT
  AAATAGAAGGCACAACAGTTTTGCTCTGGTTGTTATCTGGGTTTTGGGAATTTTTTTGCT
  TTTTGTTTTTTTACAAATACAAATTAAACATGAAAAAACTACTTCAAAAAAACTAACAGT
  TCAAGAAAAGCCTATCAGTTGCATTCTAGACATTTAAAACCTATTATCACAATTTAAATT
  TCAATGGTAAAAACAGTTAGGTTTTAGAATTGTTTTGTGATCCCTTATCAAAAAAAAATC
  TATTCCTTTNNATTCAGTTTTAAACATACCAGTGAGAATCAAATTTGTAAATTTTTCAAT
  GGCATTTCTCCACACAAAAGTAGTCATGACTCTCAAAGAAGAACATTTGGGGTTTTCCTG
  CCTCAAAAATTATCAAACCTGTATTCTTACAGGCTTTCAACACGTAAATATAAAAATTTC
  CATGGATGAACTACCAATGGCGTATATGCAAACTCCTGGGGGAATGCTGAAAAAAAAAAA
  AACACTCGTTTGCAAGAACAAGGATCAACTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005754
• Insert Size: 3030 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005754.2, NP_005745.1
• RefSeq Size: 2855 bp
• RefSeq ORF: 1401 bp
• Locus ID: 10146
• UniProt ID: Q13283
• Cytogenetics: 5q33.1
• Domains: RRM, NTF2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.