MTF1 (NM_005955) Human Untagged Clone

CAT#: SC116396

MTF1 (untagged)-Human metal-regulatory transcription factor 1 (MTF1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MTF1
• Synonyms: MTF-1; ZRF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116396 sequence for NM_005955 edited (data generated by NextGen Sequencing)
  ATGGGGGAACACAGTCCAGACAACAACATCATCTACTTTGAGGCAGAGGAAGATGAGCTG
  ACCCCCGATGATAAAATGCTCAGGTTTGTGGATAAAAACGGACTGGTGCCTTCCTCATCT
  GGAACTGTTTATGATAGGACCACTGTTCTTATTGAGCAGGACCCTGGCACTTTGGAGGAT
  GAAGATGACGACGGACAGTGCGGAGAACACTTGCCTTTTCTAGTAGGGGGTGAAGAGGGC
  TTTCACCTGATAGATCATGAAGCAATGTCCCAGGGTTATGTGCAGCACATTATCTCACCA
  GATCAGATTCATTTGACAATAAACCCTGGTTCCACACCCATGCCAAGAAATATTGAAGGT
  GCAACCCTCACTCTGCAGTCGGAATGTCCGGAAACAAAACGTAAAGAAGTAAAGCGGTAC
  CAATGTACCTTTGAGGGCTGTCCCCGCACCTACAGCACAGCAGGCAACCTGCGAACCCAC
  CAGAAGACTCACCGAGGAGAGTACACCTTTGTCTGTAATCAGGAGGGCTGTGGCAAAGCC
  TTCCTTACCTCTTACAGCCTCAGGATCCACGTGCGAGTGCACACGAAGGAGAAGCCATTT
  GAGTGTGACGTGCAGGGCTGTGAGAAGGCATTCAACACACTGTACAGGCTGAAAGCACAT
  CAGAGGCTTCACACAGGGAAAACGTTTAACTGTGAATCTGAAGGCTGCAGCAAATACTTC
  ACCACACTCAGTGATCTGAGGAAGCACATTCGAACTCATACAGGGGAAAAGCCATTTCGG
  TGCGATCACGATGGCTGTGGAAAAGCATTTGCAGCAAGCCACCACCTTAAAACTCACGTT
  CGTACACATACTGGTGAAAGACCCTTCTTCTGCCCCAGTAATGGCTGTGAGAAAACATTC
  AGCACTCAATACAGTCTCAAAAGTCACATGAAAGGTCATGATAACAAAGGACACTCATAC
  AATGCACTTCCACAACACAATGGATCAGAGGATACAAATCACTCACTTTGTCTAAGTGAC
  TTGAGCCTTCTGTCCACAGATTCTGAATTGCGAGAAAATTCCAGTACGACCCAGGGCCAG
  GACCTCAGCACAATTTCACCAGCAATCATCTTTGAATCAATGTTCCAGAATTCAGATGAT
  ACGGCAATTCAGGAAGATCCTCAACAGACAGCTTCCTTGACTGAAAGTTTTAATGGTGAT
  GCAGAGTCAGTCAGTGATGTTCCGCCATCCACAGGAAATTCAGCATCTTTATCTCTTCCA
  CTTGTACTGCAACCTGGCCTCTCCGAGCCACCCCAGCCTCTACTACCTGCCTCAGCTCCG
  TCTGCTCCTCCGCCTGCTCCCTCCCTAGGACCTGGCTCCCAGCAAGCTGCATTTGGCAAC
  CCCCCTGCTCTCTTACAACCTCCAGAAGTGCCTGTTCCCCACAGCACACAGTTTGCTGCT
  AATCATCAAGAGTTTCTTCCGCACCCCCAGGCACCGCAGCCCATTGTACCAGGACTTTCT
  GTTGTTGCTGGGGCTTCTGCATCAGCAGCGGCAGTGGCATCAGCTGTGGCAGCACCAGCC
  CCACCACAAAGTACTACTGAGCCCCTGCCAGCCATGGTCCAGACTCTGCCCCTGGGTGCC
  AACTCTGTCCTAACTAATAATCCCACAATAACCATCACCCCAACTCCCAACACAGCTATC
  CTGCAGTCCAGCCTAGTCATGGGAGAACAGAACTTACAATGGATATTAAATGGTGCCACC
  AGTTCTCCACAAAACCAAGAACAAATTCAGCAAGCATCTAAAGTTGAGAAGGTGTTTTTT
  ACCACTGCAGTACCAGTAGCCAGTAGCCCAGGGAGCTCTGTCCAGCAGATTGGCCTCAGT
  GTTCCTGTGATCATCATCAAACAAGAAGAGGCATGTCAGTGTCAGTGTGCATGCCGGGAC
  TCTGCAAAGGAGCGGGCATCCAGCAGGAGAAAGGGCTGCTCCTCCCCACCCCCTCCAGAG
  CCGAGCCCCCAGGCTCCTGATGGGCCCAGCCTGCAGCTCCCAGCGCAGACTTTCTCTTCA
  GCCCCTGTTCCCGGGTCATCATCCTCTACCTTGCCCTCCTCCTGTGAGCAAAGCCGACAA
  GCAGAGACTCCTTCAGACCCTCAGACAGAAACATTAAGTGCCATGGATGTGTCAGAGTTT
  CTATCCCTCCAGAGCCTGGACACCCCGTCCAATCTGATTCCCATTGAAGCACTACTGCAG
  GGGGAGGAGGAGATGGGCCTCACCAGCAGCTTCTCCAAGTGA
  
  Clone variation with respect to NM_005955.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005955 unedited
  ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGGGAGGGGAGAA
  GCTGCTGCTGCCGCCGTTGCCGGGAGCCGCGGAGACAAGTCATTACGTTTTCATTTCTCA
  CAACTGGGCTGAGCACAACTGAACCATGGGGGAACACAGTCCAGACAACAACATCATCTA
  CTTTGAGGCAGAGGAAGATGAGCTGACCCCCGATGATAAAATGCTCAGGTTTGTGGATAA
  AAACGGACTGGTGCCTTCCTCATCTGGAACTGTTTATGATAGGACCACTGTTCTTATTGA
  GCAGGACCCTGGCACTTTGGAGGATGAAGATGACGACGGACAGTGCGGAGAACACTTGCC
  TTTTCTAGTAGGGGGTGAAGAGGGCTTTCACCTGATAGATCATGAAGCAATGTCCCAGGG
  TTATGTGCAGCACATTATCTCACCAGATCAGATTCATTTGACAATAAACCCTGGTTCCAC
  ACCCATGCCAAGAAATATTGAAGGTGCAACCCTCACTCTGCAGTCGGAATGTCCGGAAAC
  AAAACGTAAAGAAGTAAAGCGGTACCAATGTACCTTTGAGGGCTGTCCCCGCACCTACAG
  CACAGCAGGCAACCTGCGAACCCACCAGAAGACTCACCGAGGAGAGTACACCTTTGTCTG
  TAATCAGGAGGGCTGTGGCAAAGCCTTCCTTACCTCTTACAGCCTCANGATCCACGTGCG
  AGTGCACACGANAGAGAAGCCATTTGAGTGTGACGTGCAGGGCTGTTGAGAGGCATTCAA
  CACACTGTACAGGCTGAAAGCACATCAGAGGCTTCACACAGGGAAAAACGTTACTGTGAA
  TCTGAAGGCTGCAGCAAATACTTCACCACACTCAGTGATCTGTAGAAGCACATTCGACCT
  CATACAGGGGAAAAGCCATTTTCGTGCGATCACGATGGCTGTGGNAAAGCATTTGCAGCA
  AGCCCACACCTTAAAACTCACGNTCGTACCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005955 unedited
  CGGCCGCAATCTAGAGTCGAGNNNTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT
  TGGTTTTTTTTTTGGTTTTTTTTTTTTGGTTTTTTTCAAAAAATAATTCCTTAAAAAGGA
  TGCTCCTGGGATGGGAATAAAAAAAATACCTCTGAAAGGGGAGGATTTCAAACAGTAAAA
  TTCTTTATCCTTTAAAATTCTGACCCATGATGGGAGGCATTGGACCTCATGCCTCCTGGT
  CACCCGCTTTTTCCAAAAGGGAACACACATGGGCCCTTTACTTGGAAAAACTGGTGGTGA
  GGGCCATTTTCTTCTTCCCCTGGAGTAGTGCTTCAATGGGAATCAAATTGGACCGGGTGT
  CCAAGCTCTGGAGGGATAAAAACTCTGACACATCCCTGGCACTTAATGGTTCTGTCTGAA
  GGTCTGAAAGAATCTCTGCTTGTCGGCTTTTCTCACAAGAGGAGGGCAAGGTAAAAGATG
  ATGACCCCGGAACAGGGGCTGAAGAAAAAATCTGCGCTGGGAACTGCAAGCTGGGCCCAT
  CAAGAACCTGGGGGCTCGGCTCTTGAGGGGGTGGGGAGGAACAACCCTTTCTTCTGCTGG
  ATGCCCGCTCCTTTGCAGAGTCCCGGCATGCACACTGACACTGACATGCCTCTTCTTGTT
  TGATGATGATCACAAGAACACTGAAGCCACATCTGCTGGACAGAACTCCCTGGGCTACTG
  GCTACTGGTACTGCAGNGGGAAAAACACCTTCTCAACTTTAGAAGCCTGCTGAAATTNGT
  NCTTGGGTTTGTGGAGAACTGGTGGCACCATTTAATATCACTTGGAAGTTCTGGTCTCCA
  TGACANNNGCTGACTGCAGATAACTTGNTGGGAGTTGGGGTGATTGTTATTGGGGGAATA
  TATAANTAGGCACGNAGTTGGCACCAGGGNCACAATCTGGACCATGGCCTGCAGGGCTCA
  TANAACTTTTGGNGNGGGGGGCTTGGGCTGCCCACCTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005955
• Insert Size: 2980 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005955.1, NP_005946.1
• RefSeq Size: 3302 bp
• RefSeq ORF: 2262 bp
• Locus ID: 4520
• UniProt ID: Q14872
• Cytogenetics: 1p34.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008]