alpha Tubulin (TUBA1A) (NM_006009) Human Untagged Clone

CAT#: SC116352

TUBA1A (untagged)-Human tubulin, alpha 1a (TUBA1A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha Tubulin
• Synonyms: B-ALPHA-1; LIS3; TUBA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116352 sequence for NM_006009 edited (data generated by NextGen Sequencing)
  ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCAGATTGGCAATGCCTGC
  TGGGAGCTCTACTGCCTGGAACACGGCATCCAGCCCGATGGCCAGATGCCAAGTGACAAG
  ACCATTGGGGGAGGAGATGATTCCTTCAACACCTTCTTCAGTGAGACGGGGGCTGGCAAG
  CATGTGCCCCGGGCAGTGTTTGTAGACTTGGAACCCACAGTCATTGATGAAGTTCGCACT
  GGCACCTACCGCCAGCTCTTCCACCCTGAGCAACTTATCACAGGCAAGGAAGATGCTGCC
  AATAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCATTGACCTCGTGTTGGAC
  CGAATTCGCAAGCTGGCCGACCAGTGCACGGGTCTCCAGGGCTTCTTGGTTTTCCACAGC
  TTTGGTGGGGGAACTGGTTCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGAT
  TATGGCAAGAAGTCCAAGCTGGAGTTCTCTATTTACCCGGCGCCCCAGGTTTCCACAGCT
  GTAGTTGAGCCCTACAACTCCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGT
  GCCTTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAGAAACCTCGATATTGAG
  CGTCCAACCTATACTAACCTGAATAGGTTAATAGGTCAAATTGTGTCCTCCATCACTGCT
  TCCCTGAGATTTGATGGAGCCCTGAATGTTGACCTGACAGAATTCCAGACCAACCTGGTG
  CCCTATCCCCGCATCCACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAA
  GCCTACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCAGCCAAC
  CAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGTTGTACCGT
  GGTGACGTGGTTCCCAAAGATGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGTACC
  ATCCAGTTTGTGGATTGGTGCCCCACTGGCTTCAAGGTTGGCATCAACTACCAGCCTCCC
  ACTGTGGTGCCTGGTGGAGACCTGGCCAAGGTACAGAGAGCTGTGTGCATGCTGAGCAAC
  ACCACAGCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATGTATGCC
  AAACGTGCCTTTGTTCACTGGTACGTTGGGGAGGGGATGGAGGAAGGTGAGTTTTCAGAG
  GCCCGTGAGGACATGGCTGCCCTTGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTT
  GAAGGAGAGGGTGAGGAAGAAGGAGAGGAATACTAA
  
  Clone variation with respect to NM_006009.2
  288 a=>g;453 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006009 unedited
  GGTGTTCGANNATTTTCGTGATGACGGACTGTACTTAGAGGGNNCGGCCGCGCAATTCGG
  CACGAGGCTTACATCGACCGCCTAAGTAGTCGTGCTGTCAAGAAGCAACAACCGTCTCCT
  CTTCGTCTTCGCCATCAGCTCGGCAGACGCGAAGCAGCAACCATGCGTGAGTGCATCTCC
  ATCCACGTTGGCCAGGCTGGTGTCACAGATTGGCAATGCCTGCTGGGAGCTCTACTGCCT
  GGAACACGGCATCCAGCCCGTATGGCCAGATGCCAAGAGACAAGACCATTGAGGGAGGAG
  ATGATTCCTTCAACACCTTCTTTAAAGAGACGGGGGCTGGCAAGCATGTGCCCCGGGCAG
  TGTTTGCACACTTGGAACCCACAGTCATTGATGAAGGTCGCACTGGCACCTACCGTCAGC
  TCTTCCACCCTGAGCAACTTATCACAGGCAACGAAGATGCTGCCAATAACTATGCCCGAG
  GGCACTACACCATTGGCAAGGAGATCATTGACCTCGTGTAGGACCGAATTCGCAAGATGG
  CCGACCAGTGCACGGGTCTCCAGGGCTTCTTGGCTTTCCACAGCTTTGGTGGGGGAACTG
  ATTCTGGGTCCACCTACCTGCTCATGGAAAGTGTCTCAGTTGATTATGGTAAGAAGATCA
  AGGTGGAGTTCTCTATTTACCCGGCGCACCAGGTTTTCACAGCTGTAGTTGAGCACTAAA
  CTGCATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTTATGGTAGACAA
  TGAAGGCCTTTTATGACATCTGTCGTAGAAACCTCGATATTGAGCGTGCAACCTATACTA
  ACCTGAATAGGGTAATACGTCCAAATTGTGCCCTCCATAACTGCGTCTCCTGAAGATTGT
  ATGGAGCCCTGAATGTTGACCTGGAAGCATTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006009 unedited
  GCACTTCCAGGGCCAGNATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGT
  TCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTC
  ATTTAAGACAGGGAATACTTTATTCAAAACCCATCACAGAAATGGACAGCTTGGGTCTGT
  AACAAAGCATTCATGTTTTAAAGCATAGGTCAGTAATTGTATATGAGAGCATACACTGCT
  ACATACAAATTAACTGATCAGACCACAACTTTTCAATGTTTAAAACAGAATAAGCTTCCC
  TGTAAAAGCAGCACCTTTGTGACGTTTTAACTTTAGTATTCCTCTCCTTCTTCCTCACCC
  TCTCCTTCAACAGAATCCACACCAACCTCCTCATAATCCTTCTCAAGGGCAGCCATGTCC
  TCACGGGCCTCTGAAAACTCACCTTCCTCCATCCCCTCCCCAACGTACCAGTGAACAAAG
  GCACGTTTGGCATACATCAGGTCAAACTTGTGGTCCAGGCGAGCCCAGGCCTCAGCAATG
  GNCTGTGGTGTTGCTCAGCATGCACACAGCTCTCTGTACCTTGGCCAGGTCTCCCCAGGC
  ACCACAGTGGGAGGCTGGTAGTTGATGCCAACCTTGAAGCCAGTGGGGCACCAATCCACA
  AACTGGATGGTACGCTTGGTCTTGATGGGTGGCATGGCAGCATTGACATCTTTGGGAACC
  ACGTCACCACGTACAACAGGCAGCAGCATGTATTTACATGGCNAGGGTACATTTCACATC
  TGGTTGGCTGGCTCAAGCAGCATGGGGGATCTCTGTCCGAAAGCTGTCATGGGAAGCTTT
  CTAACAAAAGACAGGGCCTATTTGGCAAAGGAAATGGTTGCGGATAAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006009
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006009.2, NP_006000.2
• RefSeq Size: 1677 bp
• RefSeq ORF: 1356 bp
• Locus ID: 7846
• UniProt ID: Q71U36
• Cytogenetics: 12q13.12
• Domains: tubulin
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• Gene Summary: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) represents the shortest transcript and encodes isoform 1. Variants 1 and 2 encode the same protein (isoform 1) but use distinct start codons.