Plzf (ZBTB16) (NM_006006) Human Untagged Clone

CAT#: SC116350

ZBTB16 (untagged)-Human zinc finger and BTB domain containing 16 (ZBTB16), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Plzf
• Synonyms: PLZF; ZNF145
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116350 sequence for NM_006006 edited (data generated by NextGen Sequencing)
  ATGGATCTGACAAAAATGGGCATGATCCAGCTGCAGAACCCTAGCCACCCCACGGGGCTA
  CTGTGCAAGGCCAACCAGATGCGGCTGGCCGGGACTTTGTGCGATGTGGTCATCATGGTG
  GACAGCCAGGAGTTCCACGCCCACCGGACGGTGCTGGCCTGCACCAGCAAGATGTTTGAG
  ATCCTCTTCCACCGCAATAGTCAACACTATACTTTGGACTTCCTCTCGCCAAAGACCTTC
  CAGCAGATTCTGGAGTATGCATATACAGCCACGCTGCAAGCCAAGGCGGAGGACCTGGAT
  GACCTGCTGTATGCGGCCGAGATCCTGGAGATCGAGTACCTGGAGGAACAGTGCCTGAAG
  ATGCTGGAGACCATCCAGGCCTCAGACGACAATGACACGGAGGCCACCATGGCCGATGGC
  GGGGCCGAGGAAGAAGAGGACCGCAAGGCTCGGTACCTCAAGAACATCTTCATCTCGAAG
  CATTCCAGCGAGGAGAGTGGGTATGCCAGTGTGGCTGGACAGAGCCTCCCTGGGCCCATG
  GTGGACCAGAGCCCTTCAGTCTCCACTTCATTTGGTCTTTCAGCCATGAGTCCCACCAAG
  GCTGCAGTGGACAGTTTGATGACCATAGGACAGTCTCTCCTGCAGGGAACTCTTCAGCCA
  CCTGCAGGGCCCGAGGAGCCAACTCTGGCTGGGGGTGGGCGGCACCCTGGGGTGGCTGAG
  GTGAAGACGGAGATGATGCAGGTGGATGAGGTGCCCAGCCAGGACAGCCCTGGGGCAGCC
  GAGTCCAGCATCTCAGGAGGGATGGGGGACAAGGTTGAGGAAAGAGGCAAAGAGGGGCCT
  GGGACCCCGACTCGAAGCAGCGTCATCACCAGTGCTAGGGAGCTACACTATGGGCGAGAG
  GAGAGTGCCGAGCAGGTGCCACCCCCAGCTGAGGCTGGCCAGGCCCCCACTGGCCGACCT
  GAGCACCCAGCACCCCCGCCTGAGAAGCATCTGGGCATCTACTCCGTGTTGCCCAACCAC
  AAGGCTGACGCTGTATTGAGCATGCCGTCTTCCGTGACCTCTGGCCTCCACGTGCAGCCT
  GCCCTGGCTGTCTCCATGGACTTCAGCACCTATGGGGGGCTGCTGCCCCAGGGCTTCATC
  CAGAGGGAGCTGTTCAGCAAGCTGGGGGAGCTGGCTGTGGGCATGAAGTCAGAGAGCCGG
  ACCATCGGAGAGCAGTGCAGCGTGTGTGGGGTCGAGCTTCCTGATAACGAGGCTGTGGAG
  CAGCACAGGAAGCTGCACAGTGGGATGAAGACGTACGGGTGCGAGCTCTGCGGGAAGCGG
  TTCCTGGATAGTTTGCGGCTGAGAATGCACTTACTGGCTCATTCAGCGGGTGCCAAAGCC
  TTTGTCTGTGATCAGTGCGGTGCACAGTTTTCGAAGGAGGATGCCCTGGAGACACACAGG
  CAGACCCATACTGGCACTGACATGGCCGTCTTCTGTCTGCTGTGTGGGAAGCGCTTCCAG
  GCGCAGAGCGCACTGCAGCAGCACATGGAGGTCCACGCGGGCGTGCGCAGCTACATCTGC
  AGTGAGTGCAACCGCACCTTCCCCAGCCACACGGCTCTCAAACGCCACCTGCGCTCACAT
  ACAGGCGACCACCCCTACGAGTGTGAGTTCTGTGGCAGCTGCTTCCGGGATGAGAGCACA
  CTCAAGAGCCACAAACGCATCCACACGGGTGAGAAACCCTACGAGTGCAATGGCTGTGGC
  AAGAAGTTCAGCCTCAAGCATCAGCTGGAGACGCACTATAGGGTGCACACAGGTGAGAAG
  CCCTTTGAGTGTAAGCTCTGCCACCAGCGCTCCCGGGACTACTCGGCCATGATCAAGCAC
  CTGAGAACGCACAACGGCGCCTCGCCCTACCAGTGCACCATCTGCACAGAGTACTGCCCC
  AGCCTCTCCTCCATGCAGAAGCACATGAAGGGCCACAAGCCCGAGGAGATCCCGCCCGAC
  TGGAGGATAGAGAAGACGTACCTCTACCTGTGCTATGTGTGA
  
  Clone variation with respect to NM_006006.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006006 unedited
  TTCCCCGCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAG
  AGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAT
  TCGGCACGAGGGGCGATGAGAGCGGGTACTGCGAACTGCCGGGCGATGCTGTCGCTGCCG
  CCGTGATACGGAGAGCAACAGTTCCCCAGCAACACCCCTCCCCGACACAGGCACACACCC
  CCCGACAGGCACGCACACCCACCCCACAGTGCCCGGCTCGGCTGCGCCTCCTCTATTGGC
  CCAGGAAGCCCACCCAGCCCCGCCACGCAGAGCCCAGAAGGAAAGAAAGCCTCATGCCTG
  AGCCGAGGGGAGCACCATGGATCTGACAAAAATGGGCATGATCCAGCTGCAGAACCCTAG
  CCACCCCACGGGGCTACTGTGCAAGGCCAACCAGATGCGGCTGGCCGGGACTTTGTGCGA
  TGTGGTCATCATGGTGGACAGCCAGGAGTTCCACGCCCACCGGACGGTGCTGGCCTGCAC
  CAGCAAGATGTTTGAGATCCTCTTCCACCGCAATAGTCAACACTATACTTTGGACTTCCT
  CTCGCCAAAGACCTTCCAGCAGATTCTGGAGTATGCATATACAGCCACGCTGCAAGCCAA
  GGCGGAGGACCTGGATGACCTGCTGTATGCGGCCGAGATCCTGGAGATCGAGTACCTGGN
  AGAACAGTGCCTGAAGATGCTGGAGACCATNNCAGCCTCAGACGATCATGACACGGGAGC
  CACCATGGCCGATGGCGGNGCCGAGAATGATAAGACCCGCAGGGCTCGTACCTCAGAAAC
  ATCTCATCTCGAACCATTCANCGAGGANAGTGGGTATGCCAGTGTGGCTGGACAGGAGCC
  TCCTGGGCCAATGGTAGGACCAGAGCCCTTAGTCTCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006006 unedited
  ATTTACTAGTCCTTCCTCTCTCACTCCACTCTTCTTTCCTGGCTCCCCGCTCGGCTCCAC
  CGCCGCGGGCGCTCCCTTCACACATAGCACAGGTAGAGGTACGTCTTCTCTATCCTCCAG
  TCGGGCGGGATCTCCTCGGGCTTGTGGCCCTTCATGTGCTTCTGCATGGAGGAGAGGCTG
  GGGCAGTACTCTGTGCAGATGGTGCACTGGTAGGGCGAGGCGCCGTTGTGCGTTCTCAGG
  TGCGGGAGCATGGCCGAGTAGTCCCGGGAGCGCTGGTGGCAGAGCTTACACTCAAAGGGC
  TTCTCACCTGTGTGCACCCTATAGTGCGTCTCCAGCTGATGCTTGAGGCTGAACTTCTTG
  CCACAGCCATTGCACTCGTAGGGTTTCTCACCCGTGTGGATGCGTTTGTGGCTCTTGAGT
  GTGCTCTCATCCCGGAAGCAGCTGCCACACAACTCACACTTCTAGGGGTGGTCGCCTGTA
  TGTGAGCGCACCTGGCGCCTGAGAGCCTTGCTGCTTGTGAACGGGCAGTTTCCCTCACAC
  CAAATGTTCCTCCCCACCACCCCCTGCGCTTCCTTCTCCCCCCCCCAACGCCCCCCGCCC
  CCCCGGCCTCTTTGTCTTCGTTCTCACCTCACACCCTCCCGCCCCTCTTACATATCCTCT
  CCTCTACTCCCCCCCCCACCCTCCCCCTCCACCAACCCGTATTCTCCACAATCTTTACCC
  ACATCCCCGACCGCCAAACTATACACTTAATGCCCCATCTTCACGCCCCCATCCCCTCCA
  CCCCCCCCCCCCCCCCCACTCCTCGTTCGCACGACTTAGCCTTTCTGTTTGTACCTATCA
  TACACTCCCCACCCTCCCTCCATACCCCCCCCCTTCCCTCCCCCCCCCAATCCCTCTCCC
  GACTGTAATTTGTCATCCTCTACTGGTGCCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006006
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006006.4, NP_005997.2
• RefSeq Size: 2417 bp
• RefSeq ORF: 2022 bp
• Locus ID: 7704
• UniProt ID: Q05516
• Cytogenetics: 11q23.2
• Domains: BTB, zf-C2H2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• Gene Summary: This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein.