CITED2 (NM_006079) Human Untagged Clone

CAT#: SC116345

CITED2 (untagged)-Human Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 (CITED2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CITED2
• Synonyms: ASD8; MRG-1; MRG1; P35SRJ; VSD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006079 edited
  GCCTCGTGCCGAATTCGGCACGAGGCTGCTGAGCCGGTCCTGGACGCGACGAGCCCGCCC
  TCGGTCTTCGGAGCAGAATTCGCAAAAACGGAAGGACTGGAAATGGCAGACCATATGATG
  GCAATGAACCACGGGCGCTTCCCCGACGGCACCAATGGGCTGCACCATCACCCTGCCCAC
  CGCATGGGCATGGGGCAGTTCCCGAGCCCCCATCACCACCAGCAGCAGCAGCCCCAGCAC
  GCCTTCAACGCCCTAATGGGCGAGCACATACACTACGGCGCGGGCAACATGAATGCCACG
  AGCGGCATCAGGCATGCGATGGGGCCGGGGACTGTGAACGGAGGGCACCCCCCGAGCGCG
  CTGGCCCCCGCGGCCAGGTTTAACAACTCCCAGTTCATGGGTCCCCCGGTGGCCAGCCAG
  GGAGGCTCCCTGCCGGCCAGCATGCAGCTGCAGAAGCTCAACAACCAGTATTTCAACCAT
  CACCCCTACCCCCACAACCACTACATGCCGGATTTGCACCCTGCTGCAGGCCACCAGATG
  AACGGGACAAACCAGCACTTCCGAGATTGCAACCCCAAGCACAGCGGCGGCAGCAGCACC
  CCCGGCGGCTCGGGCGGCAGCAGCACCCCCGGCGGCTCTGGCAGCAGCTCGGGCGGCGGC
  GCGGGCAGCAGCAACAGCGGCGGCGGCAGCGGCAGCGGCAACATGCCCGCCTCCGTGGCC
  CACGTCCCCGCTGCAATGCTGCCGCCCAATGTCATAGACACTGATTTCATCGACGAGGAA
  GTTCTTATGTCCTTGGTGATAGAAATGGGTTTGGACCGCATCAAGGAGCTGCCCGAACTC
  TGGCTGGGGCAAAACGAGTTTGATTTTATGACGGACTTCGTGTGCAAACAGCAGCCCAGC
  AGAGTGAGCTGTTGACTCGATCGAAACCCCGGCGAAAGAAATCAAACCCCCAACTTCTTC
  GGCGTGAATTAAAAGAAACATTCCCTTAGACACAGTATCTCACTTTTCAGATCTTGAAAG
  GTTTGAGAACTTGGAAACAAAGTAAACTATAAACTTGTACAAATTGGTTTTAAAAAAAAT
  TGCTGCCACTTTTTTTTCCTGTTTTTGTTTCGTTTTTGTAGCCTTGACATTCACCCACCT
  CCCTTATGTAGTTGAAATATCTAGCTAACTTGGTCTTTTTCGTTGTTTGTTTTTACTCCT
  TTCCCTCACTTTCTCCAGTGCTCAACTGTTAGATATTAATCTTGGCAAACTGCTTAATCT
  TGTGGATTTTGTAGATGGTTTCAAATGACTGAACTGCATTCAGATTTACGAGTGAAAGGA
  AAAATTGCATTAGTTGGTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCA
  TCTCGCTTCATTTTTTTAACTATGCATTTGAGTACAGACTAATTTTTAAAATATGCTAAA
  CTGGAAGATTAAACAGATGTGGGCCAAACTGTTCTGGATCAGGAAAGTCATACTGTTCAC
  TTTCAAGTTGGCTGTCCCCCCCGCCGCCCCCCCCACCCCCATATGTACAGATGATAATAG
  GGTGTGGAATGTCGTCAGTGGCAAACATTTCACAGATTTTTATTTTGTTTCTGTCTTCAA
  CATTTTTGACACTGTGCTAATAGTTATATTCAGTACATGAAAAGATACTACTGTGTTGAA
  AGCTTTTTAGGAAATTTTGACAGTATTTTTGTACAAAACATTTTTTTGAAAAAATACTTG
  TTAATTTATTCTATTTTAATTTGCCAATGTCAATAAAAAGTTAAGAAATAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006079 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGCCTCGTGCCGAATTCGGCACGAGGCTGCTGA
  GCCGGTCCTGGACGCGACGAGCCCGCCCTCGGTCTTCGGAGCAGAATTCGCAAAAACGGA
  AGGACTGGAAATGGCAGACCATATGATGGCAATGAACCACGGGCGCTTCCCCGACGGCAC
  CAATGGGCTGCACCATCACCCTGCCCACCGCATGGGCATGGGGCAGTTCCCGAGCCCCCA
  TCACCACCAGCAGCAGCAGCCCCAGCACGCCTTCAACGCCCTAATGGGCGAGCACATACA
  CTACGGCGCGGGCAACATGAATGCCACGAGCGGCATCAGGCATGCGATGGGGCCGGGGAC
  TGTGAACGGAGGGCACCCCCCGAGCGCGCTGGCCCCCGCGGCCAGGTTTAACAACTCCCA
  GTTCATGGGTCCCCCGGTGGCCAGCCAGGGAGGCTCCCTGCCGGCCAGCATGCAGCTGCA
  GAAGCTCAACAACCAGTATTTCAACCATCACCCCTACCCCCACAACCACTACATGCCGGA
  TTTGCACCCTGCTGCAGGCCACCAGATGAACGGGACANACCAGCACTTCCGAGATTGCAA
  CCCCAAGCACAGCGGCGGCAGCAGCACCCCCGGCGGCTCGGGCGGCAGCAGCACCCCCGG
  CGGCTCTGGCAGCAGCTCGGGCGGCGGGCGCGGCAGCAGCACAGCGGNCGGGGCGGCAGC
  GGCAGCGGCAACATGGCCGCCTCCGTGGCCCACGTNCCCGCTGCATGCTGGCCGCCCAAT
  GTCATAGACCCTGATTTCATCGACCAAGGAAGTTCCTTATGTCCTTGGTGAAAAAAAAAG
  GGGTTTGGACCGCATCAAGGACCTGCCGGAACTCTGTCTGGGGCAAAACAGTTTGATTTA
  AGACGAACTTCGGGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006079 unedited
  ACTTTTTTTGACTTGGCAAATAAATAGNATAAATTAACAAGTATTTTTTCAAAAAAAGTT
  TTGTACAAAAATACTGTCAAAATTTCCTAAAAAGCTTTCAACACAGTAGTATCTTTTCAT
  GTACTGAATATAACTATTAGCACAGTGTCAAAAATGTTGAAGACAGAAACAAAATAAAAA
  TCTGTGAAATGTTTGCCACTGACGACATTCCACACCCTATTATCATCTGTACATATGGGG
  GTGGGGGGGGCGGCGGGGGGGACAGCCAACTTGAAAGTGAACAGTATGACTTTCCTGATC
  CAGAACAGTTTGGCCCACATCTGTTTAATCTTCCAGTTTAGCATATTTTAAAAATTAGTC
  TGTACTCAAATGCATAGTTAAAAAAATGAAGCGAGATGGCAGTTTGTGCAGTAATATCTG
  CCCTTCGAAGTTCATGCAACCAACTAATGCAATTTTTCCTTTCACTCGTAAATCTGAATG
  CAGTTCAGTCATTTGAAACCATCTACAAAATCCACAAGATTAAGCAGTTTGCCAAGATTA
  ATATCTAACAGTTGAGCACTGGAGAAAGTGAGGGAAAGGAGTAAAAACAAACAACGAAAA
  AGACCAAGTTAGCTAGATATTTCAACTACATAAAGGATGTGGGTGAATGTCAATGCTACA
  AAAACGAAACATATACAGGATAAAAAAGTGGCAGCAATNTTTTTTAATACCAATTTGTAC
  AAGTTTATAGTTTACTTTGTTTCCAAGTNCTCAAACCTTTCAAGATCTGAAAAGTGAGAT
  ACTGTGTCTAAGGGATGTTTCTTTTATCCACGCCGAAGAGTTGGGGTTTTGATTCTTTCG
  CAGGGTTTGATCGAGTCACAGCTACTCTGCTGGCTGCTGTTGACACGAATCCGTCTAAAT
  CAAACTGTTTGCCCACCAAAGTTCGGCACTCCTGATGCGTCAAACCATTTTATACCAGAC
  TAAACTCCTCCCATAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006079
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006079.3, NP_006070.2
• RefSeq Size: 1930 bp
• RefSeq ORF: 813 bp
• Locus ID: 10370
• UniProt ID: Q99967
• Cytogenetics: 6q24.1
• Domains: CITED
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of hypoxia-inducible factor 1-alpha to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1).