AGPAT1 (NM_006411) Human Untagged Clone

CAT#: SC116101

AGPAT1 (untagged)-Human 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha) (AGPAT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AGPAT1
• Synonyms: 1-AGPAT1; G15; LPAAT-alpha; LPAATA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006411, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATTTGTGGCCAGGGGCATGGATGCTGCTGCTGCTGCTCTTCCTGCTGCTGCTCTTCCTGCTGCCCA
  CCCTGTGGTTCTGCAGCCCCAGTGCCAAGTACTTCTTCAAGATGGCCTTCTACAATGGCTGGATCCTCTT
  CCTGGCTGTGCTCGCCATCCCTGTGTGTGCCGTGCGAGGACGCAACGTCGAGAACATGAAGATCTTGCGT
  CTAATGCTGCTCCACATCAAATACCTGTACGGGATCCGAGTGGAGGTGCGAGGGGCTCACCACTTCCCTC
  CCTCGCAGCCCTATGTTGTTGTCTCCAACCACCAGAGCTCTCTCGATCTGCTTGGGATGATGGAGGTACT
  GCCAGGCCGCTGTGTGCCCATTGCCAAGCGCGAGCTACTGTGGGCTGGCTCTGCCGGGCTGGCCTGCTGG
  CTGGCAGGAGTCATCTTCATCGACCGGAAGCGCACGGGGGATGCCATCAGTGTCATGTCTGAGGTCGCCC
  AGACCCTGCTCACCCAGGACGTGAGGGTCTGGGTGTTTCCTGAGGGAACGAGAAACCACAATGGCTCCAT
  GCTGCCCTTCAAACGTGGCGCCTTCCATCTTGCAGTGCAGGCCCAGGTTCCCATTGTCCCCATAGTCATG
  TCCTCCTACCAAGACTTCTACTGCAAGAAGGAGCGTCGCTTCACCTCGGGACAATGTCAGGTGCGGGTGC
  TGCCCCCAGTGCCCACGGAAGGGCTGACACCAGATGACGTCCCAGCTCTGGCTGACAGAGTCCGGCACTC
  CATGCTCACTGTTTTCCGGGAAATCTCCACTGATGGCCGGGGTGGTGGTGACTATCTGAAGAAGCCTGGG
  GGCGGTGGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006411 unedited
  GGAATATGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGAGACAG
  AAGGGAGGGAGGGAGGGTGCTGGGGGGACAGCCCCCCACCATTCCTACCGCTATGGGCCC
  AACCTCCCACTCCCACCTCCCCTCCATCGGCCGGGGCTAGGACACCCCCAAATCCCGTCG
  CCCCCTTGGCACCGACACCCCGACAGAGACAGAGACACAGCCATCCGCCACCACCGCTGC
  CGCAGCCTGGCTGGGGAGGGGGCCAGCCCCCCAGGCCCCCTACCCCTCTGAGGTGGCCAG
  AATGGATTTGTGGCCAGGGGCATGGATGCTGCTGCTGCTGCTCTTCCTGCTGCTGCTCTT
  CCTGCTGCCCACCCTGTGGTTCTGCAGCCCCAGTGCCAAGTACTTCTTCAAGATGGCCTT
  CTACAATGGCTGGATCCTCTTCCTGGCTGTGCTCGCCATCCCTGTGTGTGCCGTGCGAGG
  ACGCAACGTCGAGAACATGAAGATCTTGCGTCTAATGCTGCTCCACATCAAATACCTGTA
  CGGGATCCGAGTGGAGGTGCGAGGGGCTCACCACTTCCCTCCCTCGCAGCCCTATGTTGT
  TGTCTCCAACCACCAGAGCTCTCTCGATCTGCTTGGGATGATGGAGGAACTGCCAGGCCG
  CTGTGTGCCCATTGGCAAGCGCGAGCTACTGTGGGCTTGCTCTGCCGGGCTGGCCTGCCT
  GCCTGGCAAGAGTCAATCTCATCGACCCGGAGCGCACGGGGGATGCCATCAGGGTCATGT
  CTGGGTGGTCTCCTGAGGGAACCAGAAACCACAATGGCTTCATGCTGCCCTTTCAACGTC
  GCGCCCTTCATCTTGCAATGCAGGCCCAGNTTCCCATGGACCCAAAAAAGGGCCTCCTAC
  AAGAATTCCCTGCAGAAGGAGGCGGGTTTCACCCGGAAAGGCAGGACCGCAGCTGCCCCC
  AAGCCACCGGAGGCTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006411 unedited
  GTTACTTCTTTTATTTCATTTTTTTGCTGTTATCCAGNAAATTAATAAAAACCAACCACG
  CAAAACTGGGTCCCACCCTCTCCTTTTGCTCCCAGCCTACCTCCCCAGTTGTGGGAACAG
  GTCTGGAGTGAGAGGCAGGGAGTGGCTAATGCCACCAGGAAGAAATGAAAACTGGCTCAG
  AGAGGGGGAAGCCTCAACAGAAAAAGAAATAAATTAAAAGCCCTCCTATCCCCTCCAGCC
  AGGGTTCGTTCCTTTCCCCAACTCCCCAGGGGGCAGAAGTGAGTGCAGCACCTGATGTCT
  GCTTCTTCCCCTTGTGTCTGGTGAGATGGTGCAGCAGGGCTGCAGGGGGCTGGGTGGGGT
  CATGTCCACTGAAGAACTGTACTATGGGGACAGAAAACCAGAAATGTGGAGACTGAACTG
  GTATCCCAGAGAGTGCACGACCCTGGGCATCTGGGCAAGGGCAGGCATGAGACCTCTGAA
  TTAGAAGGGTCCAGCCCCCACTGACAGGAGGCTACACTGGGAGGGAAGGTGAAGGTGCTG
  AGGAAAGCTCCCAGGATGAGCCTGGGAGTGCTTCAGGTATCAGCTTCCAGCCAGAGGGCG
  AGAAGTCCTCCTCACAAATGGATGAGTCCATTGAATCCATGGACTTTGGAGTGGGGGGGA
  TTTGTTCCAAAGAATGGATGAGTCCACTGGCCAATGTGGGGTANAGGGGTAGAGAAGACC
  CATAGGAAGAGACTCCACTGGGGATGGAATGTTCCCCTCCCTTGTGTANGCTGAGTCACT
  GGAGATGAGGGGGAGGCAACTGTCCCACAGACAAGACAGTAGGAGGTGGGGGTCAAGAGT
  GGAGACTGCACCCGAGCAGAGTCCATGGATGGGGCCATTAGGGGCAGGAGTGGCCTTTTT
  CCCATTCACTTCAGAGTTGAGATTCCAAGAGAGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006411
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006411.2, NP_006402.1
• RefSeq Size: 2242 bp
• RefSeq ORF: 852 bp
• Locus ID: 10554
• UniProt ID: Q99943
• Cytogenetics: 6p21.32
• Domains: Acyltransferase
• Protein Families: Transmembrane
• Protein Pathways: Ether lipid metabolism, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.