MAP126 (SPAG5) (NM_006461) Human Untagged Clone

CAT#: SC116082

SPAG5 (untagged)-Human sperm associated antigen 5 (SPAG5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP126
• Synonyms: DEEPEST; hMAP126; MAP126
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116082 sequence for NM_006461 edited (data generated by NextGen Sequencing)
  ATGTGGCGAGTGAAAAAACTGAGCCTCAGCCTGTCGCCTTCGCCCCAGACGGGAAAACCA
  TCTATGAGAACTCCTCTCCGTGAACTTACCCTGCAGCCCGGTGCCCTCACCAACTCTGGA
  AAAAGATCCCCCGCTTGCTCCTCGCTGACCCCATCACTGTGCAAGCTGGGGCTGCAGGAA
  GGCAGCAACAACTCATCTCCAGTGGATTTTGTAAATAACAAGAGGACAGACTTATCTTCA
  GAACATTTCAGTCATTCCTCAAAGTGGCTAGAAACTTGTCAGCATGAATCAGATGAGCAG
  CCTCTAGATCCAATTCCCCAAATTAGCTCTACTCCTAAAACGTCTGAGGAAGCAGTAGAC
  CCACTGGGCAATTATATGGTTAAAACCATCGTCCTTGTACCATCTCCACTGGGGCAGCAA
  CAAGACATGATATTTGAGGCCCGTTTAGATACCATGGCAGAGACAAACAGCATATCTTTA
  AATGGACCTTTGAGAACAGACGATCTGGTGAGAGAGGAGGTGGCACCCTGCATGGGAGAC
  AGGTTTTCAGAAGTTGCTGCTGTATCTGAGAAACCTATCTTTCAGGAATCTCCGTCCCAT
  CTCTTAGAGGAGTCTCCACCAAATCCCTGTTCTGAACAACTACATTGCTCCAAGGAAAGC
  CTGAGCAGTAGAACTGAGGCTGTGCGTGAGGACTTAGTACCTTCTGAAAGTAACGCCTTC
  TTGCCTTCCTCTGTTCTCTGGCTTTCCCCTTCAACTGCCTTGGCAGCAGATTTCCGTGTC
  AATCATGTGGACCCAGAGGAGGAAATTGTAGAGCATGGAGCTATGGAGGAAAGAGAAATG
  AGGTTTCCCACACATCCTAAGGAGTCTGAAACAGAAGATCAAGCACTTGTCTCAAGTGTG
  GAAGATATTCTGTCCACATGCCTGACACCAAATCTAGTAGAAATGGAATCCCAAGAAGCT
  CCAGGCCCAGCAGTAGAAGATGTTGGTAGGATTCTTGGCTCTGATACAGAGTCTTGGATG
  TCCCCACTGGCCTGGCTGGAAAAAGGTGTAAATACCTCCGTCATGCTGGAAAATCTCCGC
  CAAAGCTTATCCCTTCCCTCGATGCTTCGGGATGCTGCAATTGGCACTACCCCTTTCTCT
  ACTTGCTCGGTGGGGACTTGGTTTACTCCTTCAGCACCACAGGAAAAGAGTACAAACACA
  TCCCAGACAGGCCTGGTTGGCACCAAGCACAGTACTTCTGAGACAGAGCAGCTCCTGTGT
  GGCCGGCCTCCAGATCTGACTGCCTTGTCTCGACATGACTTGGAAGATAACCTGCTGAGC
  TCTCTTGTCATTCTGGAGGTTCTCTCCCGCCAGCTTCGGGACTGGAAGAGCCAGCTGGCT
  GTCCCTCACCCAGAAACCCAGGACAGTAGCACACAGACTGACACATCTCACAGTGGGATA
  ACTAATAAACTTCAGCATCTTAAGGAGAGCCATGAGATGGGACAGGCCCTACAGCAGGCC
  AGAAATGTCATGCAATCATGGGTGCTTATCTCTAAAGAGCTGATATCCTTGCTTCACCTA
  TCCCTGTTGCATTTAGAAGAAGATAAGACTACTGTGAGTCAGGAGTCTCGGCGTGCAGAA
  ACATTGGTCTGTTGCTGTTTTGATTTGCTGAAGAAATTGAGGGCAAAGCTCCAGAGCCTC
  AAAGCAGAAAGGGAGGAGGCAAGGCACAGAGAGGAAATGGCTCTCAGAGGCAAGGATGCG
  GCAGAGATAGTGTTGGAGGCTTTCTGTGCACACGCCAGCCAGCGCATCAGCCAGCTGGAA
  CAGGACCTAGCATCCATGCGGGAATTCAGAGGCCTTCTGAAGGATGCCCAGACCCAACTG
  GTAGGGCTTCATGCCAAGCAAGAAGAGCTGGTTCAGCAGACAGTGAGTCTTACTTCTACC
  TTGCAACAAGACTGGAGGTCCATGCAACTGGATTATACAACATGGACAGCTTTGCTGAGT
  CGGTCCCGACAACTCACAGAGAAACTCACAGTCAAGAGCCAGCAAGCCCTGCAGGAACGT
  GATGTGGCAATTGAGGAAAAGCAGGAGGTTTCTAGGGTGCTGGAACAAGTCTCTGCCCAG
  TTAGAGGAGTGCAAAGGCCAAACAGAACAACTGGAGTTGGAAAACAGTCGTCTAGCAACA
  GATCTCCGGGCTCAGTTGCAGATTCTGGCCAACATGGACAGCCAGCTAAAAGAGCTACAG
  AGTCAGCATACCCATTGTGCCCAGGACCTGGCTATGAAGGATGAGTTACTCTGCCAGCTT
  ACCCAGAGCAATGAGGAGCAGGCTGCTCAATGGCAAAAGGAAGAGATGGCACTAAAACAC
  ATGCAGGCAGAACTGCAGCAGCAACAAGCTGTCCTGGCCAAAGAGGTGCGGGACCTGAAA
  GAGACCTTGGAGTTTGCAGACCAGGAGAATCAGGTTGCTCACCTGGAGCTGGGTCAGGTT
  GAGTGTCAATTGAAAACCACACTGGAAGTGCTCCGGGAGCGCAGCTTGCAGTGTGAGAAC
  CTCAAGGACACTGTAGAGAACCTAACGGCTAAACTGGCCAGCACCATAGCAGATAACCAG
  GAGCAAGATCTGGAGAAAACACGGCAGTACTCTCAAAAGCTAGGGCTGCTGACTGAGCAA
  CTACAGAGCCTGACTCTCTTTCTACAGACAAAACTAAAGGAGAAGACTGAACAAGAGACC
  CTTCTGCTGAGTACAGCCTGTCCTCCCACCCAGGAACACCCTCTGCCTAATGACAGGACC
  TTCCTGGGAAGCATCTTGACAGCAGTGGCAGATGAAGAGCCAGAATCAACTCCTGTGCCC
  TTGCTTGGAAGTGACAAGAGTGCTTTCACCCGAGTAGCATCAATGGTTTCCCTTCAGCCC
  GCAGAGACCCCAGGCATGGAGGAGAGCCTGGCAGAAATGAGTATTATGACTACTGAGCTT
  CAGAGTCTTTGTTCCCTGCTACAAGAGTCTAAAGAAGAAGCCATCAGGACTCTGCAGCGA
  AAAATTTGTGAGCTGCAAGCTAGGCTGCAGGCCCAGGAAGAACAGCATCAGGAAGTCCAG
  AAGGCAAAAGAAGCAGACATAGAGAAGCTGAACCAGGCCTTGTGCTTGCGCTACAAGAAT
  GAAAAGGAGCTCCAGGAAGTGATACAGCAGCAGAATGAGAAGATCCTAGAACAGATAGAC
  AAGAGTGGCGAGCTCATAAGCCTTAGAGAGGAGGTGACCCACCTTACCCGCTCACTTCGG
  CGTGCGGAGACAGAGACCAAAGTGCTCCAGGAGGCCCTGGCAGGCCAGCTGGACTCCAAC
  TGCCAGCCTATGGCCACCAATTGGATCCAGGAGAAAGTGTGGCTCTCTCAGGAGGTGGAC
  AAACTGAGAGTGATGTTCCTGGAGATGAAAAATGAGAAGGAAAAACTCATGATCAAGTTC
  CAGAGCCATAGAAATATCCTAGAGGAGAACCTTCGGCGCTCTGACAAGGAGTTAGAAAAA
  CTAGATGACATTGTTCAGCATATTTATAAGACCCTGCTCTCTATTCCAGAGGTGGTGAGG
  GGATGCAAAGAACTACAGGGATTGCTGGAATTTCTGAGCTAA
  
  Clone variation with respect to NM_006461.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006461 unedited
  GTCACGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTGAACATGGC
  GTCCTCGACTTGGTCTGAGACGTGATAGGCCTGCCTTCTGGTTGAAGATGTGGCGAGTGA
  AAAAACTGAGCCTCAGCCTGTCGCCTTCGCCCCAGACGGGAAAACCATCTATGAGAACTC
  CTCTCCGTGAACTTACCCTGCAGCCCGGTGCCCTCACCAACTCTGGAAAAAGATCCCCCG
  CTTGCTCCTCGCTGACCCCATCACTGTGCAAGCTGGGGCTGCAGGAAGGCAGCAACAACT
  CATCTCCAGTGGATTTTGTAAATAACAAGAGGACAGACTTATCTTCAGAACATTTCAGTC
  ATTCCTCAAAGTGGCTAGAAACTTGTCAGCATGAATCAGATGAGCAGCCTCTAGATCCAA
  TTCCCCAAATTAGCTCTACTCCTAAAACGTCTGAGGAAGCAGTAGACCCACTGGGCAATT
  ATATGGTTAAAACCATCGTCCTTGTACCATCTCCACTGGGGCAGCAACAAGACATGATAT
  TTGAGGCCCGTTTAGATACCATGGCAGAGACAAACAGCATATCTTTAAATGGACCTTTGA
  GAACAGACGATCTGGTGAGAGAGGAGGTGGCACCCTGCATGGGAGACAGGTTTTCAGAAG
  TTGCTGCTGTATCTGAGAAACCTATCTTTCAGGAATCTNCGTCCCATCTCTTAGAGGAGT
  CTCCACCAAATCCCTGTTCTGAACAACTACATTGCTCCCAGGAAAGCCTGAGCAGTAGAA
  CTGGAGCTGTGCGTGAGGACTTAGTACCTTCTGAAAAGTACGCCTTCTTGCCTTCCTCTG
  TNCTCTGGCTNTCCCCTTCACTGCCTTGGCAGCAAATTCCGTGTCAATCATGTGACCCAG
  NNAGAGAAATTGTAGACATGGAGCTATGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006461 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTAAATACACTTTATTTAAATAG
  CATTTATCTCAGTTGGCTCTATGCCAGTTGGTCTTGGTATTGGGGTAAGGGGGTATTGCA
  GGTAAAAAGAGGTGAAGCAGATTCTGGCTTTCAGTTTCTTAGCTCAGAAATTCCAGCAAT
  CCCTGTAGTTCTTTGCATCCCCTCACCACCTCTGGAATAGAGAGCAGGGTCTTATAAATA
  TGCTGAACAATGTCATCTAGTTTTTCTAACTCCTTGTCAGAGCGCCGAAGGTTCTCCTCT
  AGGATATTTCTATGGCTCTGGAACTTGATCATGAGTTTTTCCTTCTCATTTTTCATCTCC
  AGGAACATCACTCTCAGTTTGTCCACCTCCTGAGAGAGCCACACTTTCTCCTGGATCCAA
  TTGGTGGCCATAGGCTGGCAGTTGGAGTCCAGCTGGCCTGCCAGGGCCTCCTGGAGCACT
  TTGGTCTCTGTCTCCGCACCCCGAAGCGAGCGGGTAAGGTGGGCCACCTTCTCTCTAAGG
  CTTATGAGCTCGCCACTCTTGCCTATCTGTTCTAGGATCTTCTCATTCTGCTGCTGTATC
  ACTTNCTGGAGCTCCTTTTCATTCTTGCACCGCAAGCCCAAGGGCCTGGCTCAGCTACCT
  TTATGCCTTGTTCCTTTTGCCTTCTGGACCTCCTGAAGCTGGTCCCTCCTGGGTCTGAAC
  CCAGCTTGCAGNTCACAAAATTTTCGCTGCAAATCCCTGAGGCTCTCTCTTCAAACCTTC
  GCCGAGGGAACCCAGAACCCGAACTTCCNGCTCTCACATCCATTTTGCACGCTCTCCCCT
  TGCCCGGGGCTTCCGGGGGCGAGGGAAACCTTTGTGGTTTTCCGGGTAACCCCTCCTCTC
  CTTTTCCCATGGCCCGGAGTTATTCTTGCCCTCTCCGCCCTGCTCACAATCCCCTCAATA
  CCCTTGCCTTCGACAGGGATATCGCGGCGGCCGCTCCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006461
• Insert Size: 4130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006461.3, NP_006452.3
• RefSeq Size: 3824 bp
• RefSeq ORF: 3582 bp
• Locus ID: 10615
• UniProt ID: Q96R06
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]