RPC62 (POLR3C) (NM_006468) Human Untagged Clone

CAT#: SC116049

POLR3C (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide C (62kD) (POLR3C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPC62
• Synonyms: C82; RPC3; RPC62
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006468, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTCAAGCAGAAATTAAGCTCTGTTCTTTGTTGCTGCAAGAGCATTTTGGAGAGATTGTAGAAAAAA
  TTGGAGTCCATCTGATAAGAACCGGCAGCCAGCCACTAAGAGTAATTGCCCATGACACAGGAACATCACT
  GGATCAGGTGAAGAAAGCCCTGTGTGTCCTCGTCCAACATAACCTGGTGAGTTATCAAGTGCACAAACGT
  GGTGTGGTGGAGTATGAAGCCCAGTGCAGCCGGGTATTGCGAATGCTTAGATATCCCCGGTACATCTATA
  CTACCAAAACTCTGTACAGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGTTGAACGGCAAACTGAC
  AATGTCAGCTGTTGTGAAGAAAGTGGCAGACCGGCTCACAGAGACCATGGAGGATGGCAAGACCATGGAC
  TATGCTGAAGTATCAAACACATTTGTGCGACTGGCAGACACACACTTTGTACAACGCTGCCCTTCGGTAC
  CTACCACTGAGAATTCAGACCCTGGGCCACCACCACCTGCCCCCACACTTGTCATTAATGAAAAGGACAT
  GTACCTGGTTCCTAAACTCAGCTTGATAGGGAAAGGTAAAAGGAGGAGATCATCTGATGAAGATGCTGCT
  GGGGAGCCCAAGGCCAAGAGACCAAAATATACTACAGATAACAAGGAGCCCATTCCAGATGATGGGATTT
  ATTGGCAGGCCAACCTTGACAGATTCCACCAACACTTCCGTGACCAAGCCATTGTGAGCGCAGTTGCTAA
  CAGGATGGACCAGACAAGCAGCGAGATTGTGCGAACCATGCTCCGAATGAGTGAGATTACCACTTCCTCT
  AGTGCTCCCTTCACCCAGCCATTGTCTTCCAATGAGATCTTCAGATCCCTACCTGTTGGCTATAACATCT
  CTAAGCAAGTTCTTGATCAGTATCTCACTCTGCTGGCAGATGATCCACTAGAGTTTGTTGGAAAGTCTGG
  CGACAGTGGTGGAGGAATGTATGTCATCAACCTCCATAAGGCATTAGCATCCCTAGCCACAGCCACTCTG
  GAGTCCGTCGTACAGGAGAGATTTGGGTCTCGCTGTGCTAGAATATTCCGTCTAGTTTTGCAGAAGAAAC
  ACATAGAGCAGAAGCAAGTGGAAGACTTTGCAATGATTCCTGCAAAGGAGGCAAAGGATATGCTATATAA
  GATGCTCTCAGAAAATTTCATGTCACTCCAGGAAATTCCCAAAACACCAGACCATGCCCCATCCAGGACC
  TTCTATTTATATACTGTGAACATCCTGTCAGCTGCCCGAATGTTGTTGCACAGGTGCTACAAGAGCATAG
  CCAACTTGATAGAAAGGAGGCAATTTGAAACCAAAGAGAATAAGCGTCTACTAGAAAAATCTCAGAGGGT
  AGAAGCCATCATTGCATCTATGCAGGCTACTGGTGCAGAGGAAGCACAGTTACAAGAAATAGAGGAGATG
  ATCACAGCTCCTGAACGTCAGCAGCTAGAGACCCTGAAACGTAATGTCAACAAGTTGGATGCCAGTGAGA
  TCCAGGTGGACGAAACCATCTTCCTGCTGGAGTCTTACATTGAGTGCACCATGAAGAGACAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006468 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCTTCGGTGGCGATC
  CGCGTCCTAGAAGGGCGGTGGGCTCCACCTCGGCCTANAAGGCCAGCGGGAGCCGTAGGA
  AGCCGTCGCGGGAAGCTCAGCCGAATTGGAGTTGGAGCCCCCGGATTGCGCTGACCCTGA
  GCTCTCAGACTCCCCAGTACAATGACTCAAGCAGAAATTAAGCTCTGTTCTTTGTTGCTG
  CAAGAGCATTTTGGAGAGATTGTAGAAAAAATTGGAGTCCATCTGATAAGAACCGGCAGC
  CAGCCACTAAGAGTAATTGCCCATGACACAGGAACATCACTGGATCAGGTGAAGAAAGCC
  CTGTGTGTCCTCGTCCAACATAACCTGGTGAGTTATCAAGTGCACAAACGTGGTGTGGTG
  GAGTATGAAGCCCAGTGCAGCCGGGTATTGCGAATGCTTAGATATCCCCGGTACATCTAT
  ACTACCAAAACTCTGTACAGTGACACTGGAGAGCTGATTGTTGAGGAGCTTCTGTTGAAC
  GGCAAACTGACAATGTCAGCTGTTGTGAAGAAAGTGGCAGACCGGCTCACAGAGACCATG
  GAGGATGGCAAGACCATGGACTATGCTGAAGTATCAAACACATTTGTGCGACTGGCAGAC
  ACACACTTTGTACAACGCTGCCCTTCGGTACCTACCACTGAGAATTCAGACCCTGGGCCA
  CCACCACCTGCCCCCACACTTGTCATTAAATGAAAGGACATGTACCTGGTTCCCTAACTC
  AGCTTGATAGGGGAAAGGTANAGGAGGAGATCATCTGATGAAGATGCTGCTGGGGAGCCC
  AAGGCCAGAGACANAATATCTACAGATACCAGGAGCCCATTCAGAGATGGGATTATTGGC
  AGGCAACCTGACAGATTCACCACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006468 unedited
  GCACGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGC
  ATCCAGGCACCTCCTTTATTTTGCTTTCCATCCCCCCAAATCTTCTGAGGAAAATGCTTC
  TTCTGGATCACTGTCTCTTCATGGGGCACTCAATGTAAAACTCCAGCAGGAAAATGGTTT
  CGTCCACCTGGATCTCACTGGCATCCAACTTGTTGACATTACGTTTCAAGGTCTCTAACT
  GCTGACGTTCAAGAGCTGGGATCATCTCCTCTATTTCTTGAAACTGGGCTTCCTCTGCAC
  CAGTAGCCTGCATAAATGCAATGATGGCTTCTACCCTCTGAAATTTTTCTAGTAAACGCT
  TATTCTCTTTGGTTTCAAATTGCCTCCTTTCTATCAAGTTGGCTATGCTCTTGTAGCACC
  TGTGCAACAACATTCGGGCAGCTGACAGGATGTTCACAGTATATAAATAAAAGGTCCTGG
  ATGGGGCATGGTCTGGTGTTTTGGGAATTTCCTGGAGTGACATGAAATTTTCTGAGAGCA
  TCTTATATAGCATATCCTTTGCCTCCTTTGCAGGAATCATTGCAAAGTCTTCCACTTGCT
  TCTGCTCTATGAGTTTCTTCTGCAAAACTAGACGGAATATTCTAGCACAGCGAGACCCAA
  ATCTCTCCTGTACGACGGACTCCANAGTGGCTGTGGCTAGGGATGCTAATGCCTTATGAG
  AGNTGATGACATACATTCCTCCACCACTGTCGCCAGACTTTCCAACAAACTCTAGTGGAT
  CATCTGCCAGCAGAGTGAGATACTGATCAAGAACTTGCTTAAAGATGTTATAGCCAACAG
  GTAGGGATCTGAAGATCTCATTGGAAGACATGGCTGGGTGAGAGGACACTANAGAAAATG
  GATCTACTCATTCGACAGGGTCGACATCTCCTGCTGNCTGGCCATCTGTTACACTGGCTA
  CAATGCTGTCACGAATTGTGTGGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006468
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006468.5, NP_006459.3
• RefSeq Size: 1889 bp
• RefSeq ORF: 1605 bp
• Locus ID: 10623
• UniProt ID: Q9BUI4
• Cytogenetics: 1q21.1
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1).