IGF2BP1 (NM_006546) Human Untagged Clone

CAT#: SC116030

IGF2BP1 (untagged)-Human insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGF2BP1
• Synonyms: CRD-BP; CRDBP; IMP-1; IMP1; VICKZ1; ZBP1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116030 sequence for NM_006546 edited (data generated by NextGen Sequencing)
  ATGAACAAGCTTTACATCGGCAACCTCAACGAGAGCGTGACCCCCGCGGACTTGGAGAAA
  GTGTTTGCGGAGCACAAGATCTCCTACAGCGGCCAGTTCTTGGTCAAATCCGGCTACGCC
  TTCGTGGACTGCCCGGACGAGCACTGGGCGATGAAGGCCATCGAAACTTTCTCCGGGAAA
  GTAGAATTACAAGGAAAACGCTTAGAGATTGAACATTCGGTGCCCAAAAAACAAAGGAGC
  CGGAAAATTCAAATCCGAAATATTCCACCCCAGCTCCGATGGGAAGTACTGGACAGCCTG
  CTGGCTCAGTATGGTACAGTAGAGAACTGTGAGCAAGTGAACACCGAGAGTGAGACGGCA
  GTGGTGAATGTCACCTATTCCAACCGGGAGCAGACCAGGCAAGCCATCATGAAGCTGAAT
  GGCCACCAGTTGGAGAACCATGCCCTGAAGGTCTCCTACATCCCCGATGAGCAGATAGCA
  CAGGGACCTGAGAATGGGCGCCGAGGGGGCTTTGGCTCTCGGGGTCAGCCCCGCCAGGGC
  TCACCTGTGGCAGCGGGGGCCCCAGCCAAGCAGCAGCAAGTGGACATCCCCCTTCGGCTC
  CTGGTGCCCACCCAGTATGTGGGTGCCATTATTGGCAAGGAGGGGGCCACCATCCGCAAC
  ATCACAAAACAGACCCAGTCCAAGATAGACGTGCATAGGAAGGAGAACGCAGGTGCAGCT
  GAAAAAGCCATCAGTGTGCACTCCACCCCTGAGGGCTGCTCCTCCGCTTGTAAGATGATC
  TTGGAGATTATGCATAAAGAGGCTAAGGACACCAAAACGGCTGACGAGGTTCCCCTGAAG
  ATCCTGGCCCATAATAACTTTGTAGGGCGTCTCATTGGCAAGGAAGGACGGAACCTGAAG
  AAGGTAGAGCAAGATACCGAGACAAAAATCACCATCTCCTCGTTGCAAGACCTTACCCTT
  TACAACCCTGAGAGGACCATCACTGTGAAGGGGGCCATCGAGAATTGTTGCAGGGCCGAG
  CAGGAAATAATGAAGAAAGTTCGGGAGGCCTATGAGAATGATGTGGCTGCCATGAGCCTG
  CAGTCTCACCTGATCCCTGGCCTGAACCTGGCTGCTGTAGGTCTTTTCCCAGCTTCATCC
  AGCGCAGTCCCGCCGCCTCCCAGCAGCGTTACTGGGGCTGCTCCCTATAGCTCCTTTATG
  CAGGCTCCCGAGCAGGAGATGGTGCAGGTGTTTATCCCCGCCCAGGCAGTGGGCGCCATC
  ATCGGCAAGAAGGGGCAGCACATCAAACAGCTCTCCCGGTTTGCCAGCGCCTCCATCAAG
  ATTGCACCACCCGAAACACCTGACTCCAAAGTTCGTATGGTTATCATCACTGGACCGCCA
  GAGGCCCAATTCAAGGCTCAGGGAAGAATCTATGGCAAACTCAAGGAGGAGAACTTCTTT
  GGTCCCAAGGAGGAAGTGAAGCTGGAGACCCACATACGTGTGCCAGCATCAGCAGCTGGC
  CGGGTCATTGGCAAAGGTGGAAAAACGGTGAACGAGTTGCAGAATTTGACGGCAGCTGAG
  GTGGTAGTACCAAGAGACCAGACCCCTGATGAGAACGACCAGGTCATCGTGAAAATCATC
  GGACATTTCTATGCCAGTCAGATGGCTCAACGGAAGATCCGAGACATCCTGGCCCAGGTT
  AAGCAGCAGCATCAGAAGGGACAGAGTAACCAGGCCCAGGCACGGAGGAAGTGA
  
  Clone variation with respect to NM_006546.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006546 unedited
  NGGGGTATTCCGCGCCCGTTGACGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
  TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG
  CCGCGAATTCGGCACGAGGGCTCCCGAGACCGCCACCATGAACAAGCTTTACATCGGCAA
  CCTCAACGAGAGCGTGACCCCCGCGGACTTGGAGAAAGTGTTTGCGGAGCACAAGATCTC
  CTACAGCGGCCAGTTCTTGGTCAAATCCGGCTACGCCTTCGTGGACTGCCCGGACGAGCA
  CTGGGCGATGAAGGCCATCGAAACTTTCTCCGGGAAAGTAGAATTACAAGGAAAACGCTT
  AGAGATTGAACATTCGGTGCCCAAAAAACAAAGGAGCCGGAAAATTCAAATCCGAAATAT
  TCCACCCCAGCTCCGATGGGAAGTACTGGACAGCCTGCTGGCTCAGTATGGTACAGTAGA
  GAACTGTGAGCAAGTGAACACCGAGAGTGAGACGGCAGTGGTGAATGTCACCTATTCCAA
  CCGGGAGCAGACCAGGCAAGCCATCATGAAGCTGAATGGCCACCAGTTGGAGAACCATGC
  CCTGAAGGTCTCCTACATCCCCGATGAGCAGATAGCACAGGGACCTGAGAATGGGCGCCG
  AGGGGGCTTTGGCTCTCGGGGTCAGCCCCGCCAGGGCTCACCTGTGGCAGCGGGGGCCCC
  AGCCAAGCAGCAGCAAGTGGACATCCCCCTTCGGCTCCTGGTGCCCACCCAGTATGTGGG
  TGCCATTATTGGCAAGGAGGGGGCCACCATCCGCAACATCACAAACAGACCCAGTCCAAG
  AAGACGTGCATAGTAAGAGCACGCAGGTGCAGCTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006546 unedited
  NCCGGAATTCTATGTACCGCGGCCGCAATCNANGATCGGATTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAAGTACCCTCCAATTTACTGAAT
  TTGCCCAAAGAAGGGACTTTAACTTTATCGGGGAGCCCAAGGGTGGGTTCCAAATCCCAC
  CCCCCAGGGAACAATGCCCTGGCTGGGAATGCCCCACAACCCCTGGCTTTTAGAACCAAC
  CCTTATGGGAACACAAGGGCTGGGGGGAAGGCACCCTGGCAACCTTTGGGGCCCCTCAAA
  TCCCCCCTGGAACCCAAACTTTTTTAAGGGGAGTTAACGGCCCCTTTCCAGGGCTCATAT
  CCCAACCTTAAAAAAAACTTTAAAAAAAAATAAAAACCCCCCGAAAACCAAGGGAAACAA
  GGACAGGGGTCAACCCCTACCCTGGTGAGCATTTAATACCCCCCATGTGCCCCCGGGCAT
  AAAGGGAAACCCACCCCGGAAAAGGACATTAATGTGGGGGGGAAACCCCCACCAAAGGGG
  GAAAATTTAATTGTGCCCATTTTAAAAAGGGGAACTGGCCTAAATTAACCCCCAATGCCC
  CCCCTTTTAAACGGGAATAATAAGGATTTTAACCCACTTTTTCCCCAATTTCCCCCCTTT
  TTCATTTCCCATTGGACCCATTGGGTAAGGAGTGATTGGAAATAAACTACCGAACCTGGC
  CGGTCCTTTCCCCTGGATGAAAACATGGAACAAAAAAAGGCTTTCCTCCAATTTTTTCTG
  GGGCCATTAAAAAAAAAAAATTTGGGAAAATAAAAAAAGCGGTAAGGGGGGGTTTTAAAC
  AATTCCCCCATTGGGGAAAAAAAATTGAAAAACTTTAGGTCCAAAAAGAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006546
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006546.3, NP_006537.3
• RefSeq Size: 8769 bp
• RefSeq ORF: 1734 bp
• Locus ID: 10642
• UniProt ID: Q9NZI8
• Cytogenetics: 17q21.32
• Domains: RRM, KH
• Gene Summary: This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.