HBS1L (NM_006620) Human Untagged Clone

SKU SC115985

HBS1L (untagged)-Human HBS1-like (S. cerevisiae) (HBS1L), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: HBS1L
• Synonyms: EF-1a; eRF3c; ERFS; HBS1; HSPC276
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115985 sequence for NM_006620 edited (data generated by NextGen Sequencing); ATGGCCCGGCATCGGAATGTTCGAGGCTATAACTACGATGAAGATTTTGAAGATGATGAT; CTCTACGGCCAGTCTGTAGAGGATGATTATTGTATTTCGCCGTCAACAGCTGCTCAGTTT; ATTTATTCACGGCGTGACAAACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGAT; CTGAAAGAATCTTCCAATTCTGTTTCAAACCATCAGCTCAGTGGATTTGATCAAGCTCGT; CTTTATTCATGCCTTGATCACATGAGAGAGGTACTTGGAGATGCTGTGCCAGATGAAATA; TTAATTGAAGCAGTTCTGAAGAACAAGTTTGATGTGCAGAAGGCTTTGTCAGGGGTTCTG; GAACAAGATAGAGTGCAGAGTTTGAAGGACAAGAATGAGGCAACAGTATCTACAGGAAAG; ATAGCAAAAGGAAAACCAGTAGATTCCCAGACATCGCGAAGTGAATCTGAAATTGTGCCA; AAAGTTGCTAAAATGACTGTATCTGGAAAGAAGCAAACTATGGGATTTGAAGTGCCTGGA; GTATCTTCTGAAGAAAATGGTCATAGTTTCCACACACCTCAAAAAGGACCGCCCATTGAA; GATGCCATTGCTTCTTCCGATGTTCTTGAGACTGCTTCTAAATCTGCTAATCCACCCCAC; ACGATTCAAGCATCAGAAGAGCAGAGTTCAACCCCAGCACCGGTGAAAAAGTCTGGCAAG; CTGAGGCAGCAAATAGATGTGAAGGCGGAACTGGAGAAGCGGCAAGGAGGGAAGCAGCTA; CTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCATATG; CTTTATCTTCTGGGTAATATAAACAAAAGAACTATGCATAAGTATGAACAGGAGTCTAAA; AAGGCTGGCAAAGCTTCGTTTGCATATGCATGGGTCTTGGATGAAACTGGCGAAGAAAGG; GAAAGGGGAGTAACCATGGATGTTGGTATGACAAAGTTTGAAACCACAACCAAAGTTATT; ACATTAATGGATGCTCCAGGCCATAAGGACTTCATTCCAAATATGATTACAGGAGCAGCC; CAGGCGGATGTAGCTGTTTTAGTTGTAGATGCCAGCAGGGGAGAGTTTGAAGCTGGATTT; GAGACTGGAGGACAAACACGAGAGCATGGACTCTTGGTCCGTTCTCTGGGAGTGACGCAG; CTTGCAGTTGCAGTTAATAAAATGGATCAGGTTAATTGGCAACAAGAAAGGTTTCAAGAG; ATTACTGGAAAACTTGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGGT; TTTATTCCTACAAGTGGTCTCAGTGGTGAAAATCTAATCACAAGATCTCAGTCAAGTGAA; CTCACAAAATGGTATAAAGGACTATGTTTATTAGAACAAATTGATTCCTTTAAGCCTCCC; CAGCGATCTATTGACAAACCTTTTAGATTATGTGTGTCCGATGTTTTCAAAGATCAAGGA; TCTGGATTTTGCATAACTGGTAAAATAGAAGCTGGTTATATCCAAACTGGTGACCGACTA; CTGGCAATGCCTCCTAATGAAACTTGTACCGTGAAAGGAATCACTCTGCATGATGAACCT; GTCGACTGGGCGGCAGCAGGCGATCATGTTAGTCTTACTTTGGTTGGGATGGATATCATC; AAAATCAATGTTGGCTGCATATTTTGTGGCCCCAAAGTACCCATTAAAGCTTGCACTCGT; TTCAGAGCCCGAATCCTCATCTTTAATATTGAAATTCCTATCACTAAAGGATTTCCTGTG; CTGTTACACTACCAAACTGTCAGTGAACCCGCCGTTATTAAACGATTGATTAGTGTCTTA; AACAAAAGCACGGGTGAAGTCACAAAGAAAAAGCCTAAGTTTTTGACTAAAGGCCAGAAT; GCATTGGTAGAGCTACAGACACAAAGACCAATAGCTCTTGAGCTATATAAAGACTTTAAA; GAGCTGGGGAGGTTCATGCTACGTTACGGTGGTTCTACAATAGCTGCTGGTGTTGTCACT; GAGATAAAAGAATGA; ; Clone variation with respect to NM_006620.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006620 unedited; TATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCGCCTTATCAGTATCTAAGCG; GAGTGTTTTGGAAGGAGTTAAGGGGCTGTGGCAAACGCCCTCTCCGCCGTCATGGCCCGG; CATCGGAATGTTCGAGGCTATAACTACGATGAAGATTTTGAAGATGATGATCTCTACGGC; CAGTCTGTAGAGGATGATTATTGTATTTCGCCGTCAACAGCTGCTCAGTTTATTTATTCA; CGGCGTGACAAACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGATCTGAAAGAA; TCTTCCAATTCTGTTTCAAACCATCAGCTCAGTGGATTTGATCAAGCTCGTCTTTATTCA; TGCCTTGATCACATGAGAGAGGTACTTGGAGATGCTGTGCCAGATGAAATATTAATTGAA; GCAGTTCTGAAGAACAAGTTTGATGTGCAGAAGGCTTTNGTCAGGGGTTCTGGAACAAGA; TAGAGTGCAGAGTTTGAAGGACAAGAATGAGGCAACAGTATCTACAGGAAAGATAGCAAA; AGGAAAACCAGTAGATTCCCAGACATCGCGAAGTGAATCTGAAATTGTGCCAAAAGTTGC; TAAATGACTGTATCTGGAAAGAAGCAACTATGGGATTTTGAAGTGCCTGGAGTATCTTCT; GAAGAAATGGTCATAGTTTCCACACCCTCANAAAGACCGCCATTNGAGATGCCATGCTCT; TTCCGATGTTCTGGAGACTGCTCCTAATCTGCTAATCCACCCACACGATCAGCATAGAGA; AANCGAGTTCACCCACCACGNGGAAAAAGTCTGCAGCTGAGACAAAAAAATTTTAAGGCG; ACTGGAAACGCAGGAGGGACACACCCCCCTAGGGCTTTGGCCTTGGAGGGGGAAAAACTC; TGGGGGCCTGCTTTTTTTTGGGAATAAAAAACTCCTATTTAAAGGGTTAAAGTGCGATTT; TTTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006620 unedited; AGCTTTGGACCGGCGGCCGCAATCTAGGATCGAGNNNTTTTTTTTTTTTTTTTTTTATTA; TGCTCAAACTAAGGCATTTTATTAGCTGGCTTTACAACTTAAATAATATCTTGGCTTTCA; AAGGAACAGCTTCCACTAATTCCAAATTAAACTTTCACAAGTTTACTTGTTTGGGGAGGG; ACATTCTTATGGTCACCACAAAATACTTTTATTATAACCTTCCCCAAATCTTTTCTTAGC; ATTAACTGGAAAAAAAAAAAAAAAAAAGCTTAGGTCAAATATCAACTGCCTGAAAAACCC; AATTAAGTTACTTTTCCTTAAAACATGTGCAGTATAATTGAATCAAAAGAGAAAACTGCA; AATACATTGTGCTTTGGCCAGAAGTAGAGTTCATTTCATGATGATTCAGTATCTTCAGAT; ACTATTTTTGACACTTGCCATAAATCTTAGCAAAGTAAATCCATTTATTAACGTTTCAAA; GGCAAAGTTGTTTTTAACATTAAACTTTCTTTGCCAGTTGGCAACTTAAAATTTTTGCAG; AGGTGATTATTAATACTTCTTTGCAGCTAATTTTAGCTTTAATTTTTCTCTCTCATCTAA; AAACATATCAATTGCACATTGTTCCTTTGACTTAATAACTGCATTCTTGAAACAGAGGTT; AGCTATTTCACTGTATCCAGAAACGTGGTAGAAATTCTGACCCATCATTCTTTTATCTCA; GTGACAACACCAGCAGCTATTGTAGAACCACCGTACGTAGCATGAACCTCCCCAGCTCTT; TAAGTCTTTATATAGCTCAAGACTATTGGTCTTTGTGTCTGTAGCTCTACCATGCATTCT; GGCCTTAGTCAAAAACTTAAGCTTTTNCTTTGTGACTTCACCCGTGCTTTGTTTAAGACA; CTATCATCGTTTATAACCGCCGGTCACTGACAGTTGGGAGTGAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_006620
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006620.2, NP_006611.1
• RefSeq Size: 7163 bp
• RefSeq ORF: 2055 bp
• Locus ID: 10767
• UniProt ID: Q9Y450
• Cytogenetics: 6q23.3
• Domains: GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3
• Summary: This gene encodes a member of the GTP-binding elongation factor family. It is expressed in multiple tissues with the highest expression in heart and skeletal muscle. The intergenic region of this gene and the MYB gene has been identified to be a quantitative trait locus (QTL) controlling fetal hemoglobin level, and this region influnces erythrocyte, platelet, and monocyte counts as well as erythrocyte volume and hemoglobin content. DNA polymorphisms at this region associate with fetal hemoglobin levels and pain crises in sickle cell disease. A single nucleotide polymorphism in exon 1 of this gene is significantly associated with severity in beta-thalassemia/Hemoglobin E. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, May 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).