RPP40 (NM_006638) Human Untagged Clone

CAT#: SC115963

RPP40 (untagged)-Human ribonuclease P/MRP 40kDa subunit (RPP40)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPP40
• Synonyms: bA428J1.3; RNASEP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115963 sequence for NM_006638 edited (data generated by NextGen Sequencing)
  ATGGCCACGTTGCGCCGGCTTCGGGAGGCGCCGCGGCACTTACTGGTTTGCGAGAAATCC
  AACTTCGGCAACCACAAGTCGCGCCACCGGCATCTTGTGCAGACGCACTACTATAACTAC
  AGGGTTTCATTTCTCATTCCTGAATGTGGGATACTATCGGAAGAACTGAAAAACCTGGTC
  ATGAACACTGGACCCTATTACTTTGTGAAGAATTTACCTCTTCATGAATTAATTACACCT
  GAATTCATCAGTACCTTTATAAAGAAAGGTTCTTGCTATGCACTAACATACAATACACAT
  ATTGATGAAGATAATACTGTTGCCCTGCTACCAAATGGGAAATTAATTTTGTCACTGGAT
  AAAGACACTTATGAAGAAACTGGACTTCAGGGTCATCCATCTCAGTTTTCTGGCAGAAAA
  ATTATGAAATTTATTGTTTCCATTGATTTGATGGAATTATCCTTAAACTTGGATTCTAAG
  AAGTATGAAAGAATATCTTGGTCTTTCAAAGAAAAGAAGCCATTGAAATTTGATTTTCTT
  TTGGCTTGGCATAAAACAGGTTCAGAAGAATCGACAATGATGTCATATTTTTCCAAGTAC
  CAAATTCAGGAGCATCAGCCAAAAGTAGCACTGAGCACGTTGAGAGATCTCCAGTGCCCA
  GTGCTGCAGAGCAGCGAGCTGGAGGGAACGCCAGAGGTGTCCTGCCGGGCTCTGGAGCTC
  TTCGACTGGCTCGGCGCCGTCTTCAGTAATGTCGACCTAAATAATGAGCCTAATAATTTC
  ATATCAACCTATTGCTGTCCTGAGCCAAGCACAGTGGTGGCAAAAGCTTATTTGTGTACA
  ATCACTGGCTTCATACTTCCAGAGAAGATCTGTCTCCTATTGGAACATCTCTGTCACTAC
  TTTGATGAACCGAAGTTAGCTCCATGGGTTACACTGTCCGTTCAAGGCTTTGCAGACAGC
  CCTGTTTCTTGGGAAAAAAATGAACATGGTTTTCGAAAAGGAGGAGAACATTTATATAAC
  TTTGTGATTTTTAATAATCAGGACTATTGGCTTCAGATGGCTGTTGGGGCAAATGATCAC
  TGTCCACCATAA
  
  Clone variation with respect to NM_006638.2
  10 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006638 unedited
  GTTCGGCATTATGTAATACGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACGAGGGG
  GGAGGACCAGGNAACCCAGNAGAGCATGGCCACGTTGCGCCGGCTTCGGGAGGCGCCGCG
  GCACTTACTGGTTTGCGAGAAATCCAACTTCGGCAACCACAAGTCGCGCCACCGGCATCT
  TGTGCAGACGCACTACTATAACTACAGGGTTTCATTTCTCATTTCTGAATGTGGGATACT
  ATCGGAAGAACTGAAAAACCTGGTCATGAACACTGGACCCTATTACTTTGTGAAGAATTT
  ACCCTCTTCATGAATTAATTACACCTGAATTCATCAGTACCTTTATAAAGAAAGGTTCTT
  GCTATGCACTAACATACAATACACATATTGATGAAGATAATACTGTTGCCCTGCTACCAA
  ATGGGAAATTAATTTTGTCACTGGATAAAGACACTTATGAAGAAACTGGACTTCAGGGTC
  ATCCATCTCAGTTTTCTGGCAGAAAAATTATGAAATTTATTGTTTCCATTGATTTGATGG
  AATTATCCTTAAACTTGGATTCTAAGAAGTATGAAAGAATATCTTGGTCTTTCAAAGAAA
  AGAAGCCATTGAAATTTGATTTTCTTTTGGCTTGGCATACAACAGGTTCAGAAGAATCGA
  CAATGATGTCATATTTTTCCAAGTACCAAATTCAGGAGCATCAGCCAAAAGTAGCACTGA
  GCACGTTGAGAGATCTCCAGTGCCCAGTGCTGCACAGCAGCGAGCTGCAGGCACGCCCAN
  AGTGTCCTGCCCGGCTCTGCAGCTCTCCGACTGGCTCGGCGCCGTTCTCACATATGTCGA
  CCTAAATATGAGCCCAATAATTTCATATCAACCTATTGCTGTTCTGAACCAACACAGTCG
  TGGCAAAAGCTTATTGGTGACAATCACTGCTTTATACCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006638 unedited
  GACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAAGTAAACACGAT
  TTTTAATTTTTATTTTTTATGGGGGACAGTGATCATTTGCCCCAACAGCCATCTGAAGCC
  AATAGTCCTGATTATTAAAAATCACAAAGTTATATAAATGTTCTCCTCCTTTTCGAAAAC
  CATGTTCATTTTTTTCCCAAGAAACAGGGCTGTCTGCAAAGCCTTGAACGGACAGTGTAA
  CCCATGGAGCTAACTTCGGTTCATCAAAGTAGTGACAGAGATGTTCCAATAGGAGACAGA
  TCTTCTCTGGAAGTATGAAGCCAGTGATTGTACACAAATAAGCTTTTGCCACCACTGTGC
  TTGGCTCAGGACAGCAATAGGTTGATATGAAATTATTAGGCTCATTATTTAGGTCGACAT
  TACTGAAGACGGCGCCGAGCCAGTCGAAGAGCTCCAGAGCCCGGCAGGACACCTCTGGCG
  TTCCCTCCAGCTCGCTGCTCTGCAGCACTGGGCACTGGAGATCTCTCAACGTGCTCAGTG
  CTACTTTTGGCTGATGCTCCTGAATTTGGTACTTGGAAAAATATGACATCATTGTCGATT
  CTTCTGAACCTGTGTTATGCCAAGCCAAAAGAAAATCAAATTTCAATGGCTTCTTTTCTT
  TGAAAGACCAAGATATTCTTTCATACCTTCTTAGATCCAAGTTTAAGGATAATTCCATCA
  AATCAATGGAAACATTAAATTTCATAATTTTTCTGCCGAAAACTGAAAGGATGACCCTGA
  ATCCACGTTTTTATAAGGGTCTTTATCAGTGACAAATTAATTTCCCATTGGAGCAGGCAA
  CCAGTTTATCTTCACCAAATGGGGATGGGATGGAAGGCTAACAAGAACCTTCTTTTAAGG
  TCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006638
• Insert Size: 1220 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006638.2, NP_006629.2
• RefSeq Size: 1174 bp
• RefSeq ORF: 1092 bp
• Locus ID: 10799
• UniProt ID: O75818
• Cytogenetics: 6p25.1
• Protein Families: Stem cell - Pluripotency
• Gene Summary: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:9630247, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).