DDX18 (NM_006773) Human Untagged Clone

CAT#: SC115885

DDX18 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX18
• Synonyms: Has1; MrDb
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115885 sequence for NM_006773 edited (data generated by NextGen Sequencing)
  ATGTCACACCTGCCGATGAAACTCCTGCGTAAGAAGATCGAGAAGCGGAACCTCAAATTG
  CGGCAGCGGAACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATCGGAAACTCAAAAT
  GGAGATGTATCTGAAGAAACAATGGGAAGTAGAAAGGTTAAAAAATCAAAACAAAAGCCC
  ATGAATGTGGGCTTATCAGAAACTCAAAATGGAGGCATGTCTCAAGAAGCAGTGGGAAAT
  ATAAAAGTTACAAAGTCTCCCCAGAAATCCACTGTATTAACCAATGGAGAAGCAGCAATG
  CAGTCTTCCAATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAAAATGGTGAATGAT
  GCTGAGCCTGATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATCTGAAGAAGAAAGT
  GCCGAGACTACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAATGATGAAGATGAG
  AGTGAGGTGCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGATACTTCGTTTGCT
  TCTCTATGTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAAGAAATGGGTTTTACA
  AACATGACTGAAATTCAGCATAAAAGTATCAGACCACTTCTGGAAGGCAGGGATCTTCTA
  GCAGCTGCAAAAACAGGCAGTGGTAAAACCCTGGCTTTTCTCATCCCTGCAGTTGAACTC
  ATTGTTAAGTTAAGGTTCATGCCCAGGAATGGAACAGGAGTCCTTATTCTCTCACCTACT
  AGAGAACTAGCCATGCAAACCTTTGGTGTTCTTAAGGAGCTGATGACTCACCACGTGCAT
  ACCTATGGCTTGATAATGGGTGGCAGTAACAGATCTGCTGAAGCACAGAAACTTGGTAAT
  GGGATCAACATCATTGTGGCCACACCAGGCCGTCTGCTGGACCATATGCAGAATACCCCA
  GGATTTATGTATAAAAACCTGCAGTGTCTGGTTATTGATGAAGCTGATCGTATCTTGGAT
  GTGGGGTTTGAAGAGGAATTAAAGCAAATTATTAAACTTTTGCCAACACGTAGACAGACT
  ATGCTCTTTTCTGCCACCCAAACTCGAAAAGTTGAAGACCTGGCAAGGATTTCTCTGAAA
  AAGGAGCCATTGTATGTTGGCGTTGATGATGATAAAGCGAATGCAACAGTGGATGGTCTT
  GAACAGGGATATGTTGTTTGTCCTTCTGAAAAGAGATTCCTTCTGCTCTTTACATTCCTT
  AAGAAGAACCGAAAGAAGAAGCTTATGGTCTTCTTTTCATCTTGTATGTCTGTGAAATAC
  CACTATGAGTTGCTGAACTACATTGATTTGCCCGTCTTGGCCATTCATGGAAAGCAAAAG
  CAAAATAAGCGTACAACCACATTCTTCCAGTTCTGCAATGCAGATTCGGGAACACTATTG
  TGTACGGATGTGGCAGCGAGAGGACTAGACATTCCTGAAGTCGACTGGATTGTTCAGTAT
  GACCCTCCGGATGACCCTAAGGAATATATTCATCGTGTGGGTAGAACAGCCAGAGGCCTA
  AATGGGAGAGGGCATGCCTTGCTCATTTTGCGCCCAGAAGAATTGGGTTTTCTTCGTTAC
  TTGAAACAATCCAAGGTTCCATTAAGTGAATTTGACTTTTCCTGGTCTAAAATTTCTGAC
  ATTCAGTCTCAGCTTGAGAAATTGATTGAAAAGAATTACTTTCTTCATAAGTCAGCCCAG
  GAAGCATATAAGTCATACATACGAGCCTATGATTCCCATTCTCTGAAACAGATCTTTAAT
  GTTAATAACCTAAATTTGCCTCAGGTTGCTCTGTCATTTGGTTTCAAGGTGCCTCCCTTC
  GTTGATCTGAACGTCAACAGTAATGAAGGCAAGCAGAAAAAGCGAGGAGGTGGTGGTGGA
  TTTGGCTACCAGAAAACCAAGAAAGTTGAGAAATCCAAAATCTTTAAACACATTAGCAAG
  AAATCATCTGACAGCAGGCAGTTCTCTCACTGA
  
  Clone variation with respect to NM_006773.3
  1617 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006773 unedited
  GTTACCGTACANAAATTTGTTAATACGAACTCACTATAGGGNCGGCCCGCGTAATTCGGC
  ACGAGGGTTACTGTGTTGGCGCACTTATTCTAGGCACTTTGTTGGGCAGAATGTCACACC
  TGCCGATGAAACTCCTGCGTAAGAAGCTCGAGAAGCGGAACCTCAAATTGCGGCAGCGGA
  ACCTAAAGTTTCAGGGGGCCTCAAATCTGACCCTATCGGAAACTCAAAATGGAGATGTAT
  CTGAAGAAAAATGGGAAGTAGAAAGGTTAAAAAATCAAAACAAAAGCCCATGAATGTGGG
  CTTATCAGAAACTCAAAATGGAGGCATGTCTTAAGAAGCAGTGGGAAATATTAAAAGTTA
  CAAAGTCTCCCCAGAAATCCACTTGTATTAACCAATGGAGAAGCAGCAATGCAGTCTTCC
  AATTCAGAATCAAAAAAGAAAAAGAAGAAAAAGAGAAAAATGGTGAATGATGCTGAGCCT
  GATACGAAAAAAGCAAAAACTGAAAACAAAGGGAAATCTGAAGAAGAAAGTGCCGAGACT
  ACTAAAGAAACAGAAAATAATGTGGAGAAGCCAGATAATGATGAAGATGAGAGTTGAGGT
  GCCCAGTCTGCCCCTGGGACTGACAGGAGCTTTTGAGGATACTNTCGTTTGCTTCTCTAT
  GTAATCTTGTCAATGAAAACACTCTGAAGGCAATAAAAGAAATGGGTTTTACAAACATGA
  CTGANATTCAGCATAAAAGTATCAGACCACTTTCTGNANNGCANGGATCTTCTAGCAGCT
  GCAAAAACANGCAGTGGTTAAACCCTGGCTTTTCTCATCCCTGCAGTTGAACTCATTGTT
  AAGTTAANGTTTCATGCCCAGATGGAACAGGAGTCCTTATTCTCTCACTACTAGAGACTA
  GCCTGCAACCTTTTTGTGTTCTTAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006773 unedited
  NGGGGGGAGGTAACGCTATGGAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTT
  TTTTTTTTTCAACATTGAAGTAATTATTTAATGACACAGGGAAAATAACATGTTATGAAA
  ACAAGCTGGTTACAAGTAGTAGGTAGATGACTTAATTTTTGATAAAAAAATTAAAAAGCA
  TAAACATGCATATAAAAATTAGATTATGTACAAAATACCAACAGTATTTACTTCTGCTCA
  GTAATTAAATATTCTTCCCTTTGTTTTTGTCTTTTAAAAAACATTATTTCTGAAAAAAAA
  AATCAGAAAAACATGATCGTGGAGAGAATTATTAAACACCAGGAATTAGGAACCCAAGAA
  GCTAAGCCAGGAGCTCAGCAGTCAAGGTTGGAAGTCCAGGACAGGTCCGACCAGGGTGAT
  GGTGGCCAAGCCCAGAAATGGAAGATTGTTCTTTTCTTTTCCTACTGGAATGTTCTTTAC
  CAGTTTTCTCTATATTCGATGTTAGCACACTTATTTAATGGGATCCTATGATTAACTGGC
  ACAACAGCAAAATGATGCTAGCAACTTTGGCTTTGAACTGCTACAAAATGTACTATCTTG
  TAGTTATATACTGTACAAAACACACTAGAAATCAAGTGCTCCTACAACGAAAAACTCATC
  TGACTAAATCACTGATTTTGCTGGTTTTTTTTCTAACTTTGTTCATTTTCAATCTTCACA
  GCAAATGTATTTTTTGTTTCAAGGCCTGGAAGAGGTATTTTTTTCTCAAAAAGGAGCTAA
  CATTAATGTGGTGCGGGGCACCGTCTGAAAGCTTGGCTTTAACTCCAAGTTAATTTGGCC
  TTGCCTTTAAGGTCTCTAAAAAGGGCAAAGCTA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006773
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006773.3, NP_006764.3
• RefSeq Size: 3790 bp
• RefSeq ORF: 2013 bp
• Locus ID: 8886
• UniProt ID: Q9NVP1
• Cytogenetics: 2q14.1
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008]