PIM2 (NM_006875) Human Untagged Clone

CAT#: SC115825

PIM2 (untagged)-Human pim-2 oncogene (PIM2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIM2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006875 edited
  ATGTTGACCAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCA
  GGAGGCAAGGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAG
  GGGGGCTTTGGCACCGTCTTCGCAGGACACCGCCTCACAGATCGACTCCAGGTGGCCATC
  AAAGTGATTCCCCGGAATCGTGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGC
  CCACTCGAAGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATC
  CGCCTGCTTGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCTTTG
  CCCGCCCAGGATCTCTTTGACTATATCACAGAGAAGGGCCCACTGGGTGAAGGCCCAAGC
  CGCTGCTTCTTTGGCCAAGTAGTGGCAGCCATCCAGCACTGCCATTCCCGTGGAGTTGTC
  CATCGTGACATCAAGGATGAGAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTC
  ATTGATTTTGGTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACA
  AGGGTGTACAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACTCCCGGCCACT
  GTCTGGTCACTGGGCATCCTCCTCTATGACATGGTGTGTGGGGACATTCCCTTTGAGAGG
  GACCAGGAGATTCTGGAAGCTGAGCTCCACTTCCCAGCCCATGTCTCCCCAGACTGCTGT
  GCCCTAATCCGCCGGTGCCTGGCCCCCAAACCTTCTTCCCGACCCTCACTGGAAGAGATC
  CTGCTGGACCCCTGGATGCAAACACCAGCCGAGGATGTACCCCTCAACCCCTCCAAAGGA
  GGCCCTGCCCCTTTGGCCTGGTCCTTGCTACCCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006875 unedited
  GTCAAATTTTNGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGATGTTGAC
  CAAGCCTCTACAGGGGCCTCCCGCGCCCCCCGGGACCCCCACGCCGCCGCCAGGAGGCAA
  GGATCGGGAAGCGTTCGAGGCCGAGTATCGACTCGGCCCCCTCCTGGGTAAGGGGGGCTT
  TGGCACCGTCTTCGCAGGACACCGCCTCACAGATCGACTCCAGGTGGCCATCAAAGTGAT
  TCCCCGGAATCGTGTGCTGGGCTGGTCCCCCTTGTCAGACTCAGTCACATGCCCACTCGA
  AGTCGCACTGCTATGGAAAGTGGGTGCAGGTGGTGGGCACCCTGGCGTGATCCGCCTGCT
  TGACTGGTTTGAGACACAGGAGGGCTTCATGCTGGTCCTCGAGCGGCCTTTGCCCGCCCA
  GGATCTCTTTGACTATATCACAGAGAAGGGCCCACTGGGTGAAGGCCCAAGCCGCTGCTT
  CTTTGGCCAAGTAGTGGCAGCCATCCAGCACTGCCATTCCCGTGGAGTTGTCCATCGTGA
  CATCAAGGATGAGAACATCCTGATAGACCTACGCCGTGGCTGTGCCAAACTCATTGATTT
  TGGTTCTGGTGCCCTGCTTCATGATGAACCCTACACTGACTTTGATGGGACAAGGGTGTA
  CAGCCCCCCAGAGTGGATCTCTCGACACCAGTACCATGCACTCCCGGCCACTGTCTGGTC
  ACTGGGCATCCTCCTCTATGACATGGTGTGTGGGGACATTCCCTTTGAGAGGGACCAGGA
  GATTCTGGAAGCTGNAGCTCACTTTCCAGCCCATGTCTCCCCAGACTGCTGTGCCCTAAT
  CCGCCGGTGCCTGGCCCCCAACCTTCTTNCCGACCTCACTGGAAGAGACCTGCTGNACCC
  CTGGNATGCAACACCAGCCGAGGATGTACCCCTCAACCCTNCAAAGNNNAGCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006875 unedited
  NGGGGAATATGTGNACCGCGCCGCATTCNANGATCGNGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGATCTCTTACTTTTATTA
  AAAAGGGGGTTTCCCCCCCTGGGTTTTTAAAAAATCCCCGCCGGAAAAAAAAGGAGGGCC
  CCCTTGGAACCCCCCCCCCCACAAAACGGGGATATTTACCTTTTAATAATTGGGCCACCC
  CCGCTCAAGAGGAGGTTGCCAAAATCTGCCAAAATTGGGGGGGGCCCCCCCAGAAAACAA
  ACAGCAAGCCTTATTTCCCCTAACCCATCCCCCAAAAAACCCTCCCTCCCATCCCAATGT
  CTGGGGGGGTCCCGGGGGGGCCATCTTCCATTCCTTCCCAAATTATGGAAATAAGGGTCT
  TCTCCCCCAAATAAAAACCCTTGGGATAACAAAATAGGGGCCCCCCCCCCCAAAAAAAAA
  AAAAAAAAAAAAAAACCCTGGGGTAACAACAGGGCATTACCCCCCCCAAAAAAAAAAAAC
  CCGGGCTGGGGCCGGGAAACCCCTTGGCCCCATTTGGGACCCCTGGCTAGGGGAAAGGGG
  CCCCTTACTAAAAAAAAAACCTCCGGGGTTTTGGAAAGAAAACCCCCCTGCCCCAGTTAG
  AACCTTTGAACCAAGTTTGTGGGGGGGGAATAAGAGGTGCCCATGTGGGGGGACCCATCC
  TCCAAATAAAAATCCCGGGGCCCAAAATGGGAACCACTTTCCCAAAATAAACTTCCTTAA
  CAAAATGAGAAGCAAGAAGTTCCCCCTCCAGAAAAGGGACCCAGGTTCTGGGGAGAAAGC
  TCCCTTGTTGGATTGGGAAGGGGACTGGGCCAAACTTGGGTTTTGTCTTCTTACCCCTGG
  ATCCCAATCCCTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006875
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006875.2, NP_006866.2
• RefSeq Size: 2101 bp
• RefSeq ORF: 936 bp
• Locus ID: 11040
• UniProt ID: Q9P1W9
• Cytogenetics: Xp11.23
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia
• Gene Summary: This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.[provided by RefSeq, Nov 2009]