RIT1 (NM_006912) Human Untagged Clone

CAT#: SC115778

RIT1 (untagged)-Human Ras-like without CAAX 1 (RIT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIT1
• Synonyms: NS8; RIBB; RIT; ROC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006912 edited
  ATGGATTCTGGAACTCGCCCAGTTGGTAGCTGCTGTAGCAGCCCCGCTGGGCTCTCACGG
  GAGTACAAACTAGTGATGCTGGGTGCTGGTGGTGTAGGGAAGAGTGCCATGACCATGCAG
  TTCATCAGCCACCGATTCCCAGAAGATCATGATCCCACCATTGAAGATGCTTATAAGATC
  AGGATCCGTATTGATGATGAGCCTGCCAATCTGGACATTTTGGATACAGCTGGACAGGCA
  GAGTTTACAGCCATGCGGGACCAGTATATGAGGGCAGGAGAAGGGTTTATCATCTGTTAC
  TCTATCACGGATCGTCGAAGTTTCCATGAAGTTCGTGAGTTTAAACAGCTTATTTATCGA
  GTCCGACGTACTGACGATACACCTGTGGTTCTTGTGGGAAACAAGTCAGACCTCAAACAG
  CTAAGACAGGTCACCAAGGAAGAAGGATTGGCCTTGGCCCGAGAATTCAGCTGTCCCTTT
  TTTGAGACATCTGCTGCATACCGCTACTATATTGATGATGTTTTCCATGCCCTTGTACGG
  GAGATACGTAGGAAAGAAAAGGAGGCAGTACTGGCCATGGAGAAAAAATCTAAGCCCAAA
  AACAGTGTATGGAAGAGGCTAAAATCACCATTCCGGAAGAAGAAAGATTCAGTAACTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006912 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAAGACGAAGTG
  CGTGACCCGACCGGCTGTGGTGTTCCAGTCCCCACTGACCAGTAGGAGCAGCAGGGCGTC
  GGCTTGTGAGGTGGCTTTTCCTCGGGGCAACCCAGGAAGGCCCCAAGAGGACAATGGATT
  CTGGAACTCGCCCAGTTGGTAGCTGCTGTAGCAGCCCCGCTGGGCTCTCACGGGAGTACA
  AACTAGTGATGCTGGGTGCTGGTGGTGTAGGGAAGAGTGCCATGACCATGCAGTTCATCA
  GCCACCGATTCCCAGAAGATCATGATCCCACCATTGAAGATGCTTATAAGATCAGGATCC
  GTATTGATGATGAGCCTGCCAATCTGGACATTTTGGATACAGCTGGACAGGCAGAGTTTA
  CAGCCATGCGGGACCAGTATATGAGGGCAGGAGAAGGGTTTATCATCTGTTACTCTATCA
  CGGATCGTCGAAGTTTCCATGAAGTTCGTGAGTTTAAACAGCTTATTTATCGAGTCCGAC
  GTACTGACGATACACCTGTGGTTCTTGTGGGAAACAAGTCAGACCTCAAACAGCTAAGAC
  AGGTCACCAAGGAAGAAGGATTGGCCTTGGCCCGAGAATTCAGCTGTCCCTTTTTTGAGA
  CATCTGCTGCATACCGCTACTATATTGATGATGTTTTCCATGCCCTTGTACGGGAGATAC
  GTANGAAAGAAAAGGAGCAGTACTGGCCATGGAGAANAAATCTAAGCCAAAACAGTGTAT
  GGAAGAAGCTAANATCACCATTCCGGAAGAAGAAAGATTCAGTAACTTGAAGAGAGATGT
  GAAGTGNTTATCTGTGAACTGCAGTGCTGTATCAAAGCAGTCCAGTAACCTGCAGTACTG
  AGTATGGTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006912
• Insert Size: 2640 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006912.3, NP_008843.1
• RefSeq Size: 1104 bp
• RefSeq ORF: 660 bp
• Locus ID: 6016
• UniProt ID: Q92963
• Cytogenetics: 1q22
• Domains: ras, RAN, RAS, RHO, RAB
• Gene Summary: This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR and has multiple coding region differences. These difference cause translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.