Tubulin Polymerization Promoting Protein (TPPP) (NM_007030) Human Untagged Clone

CAT#: SC115750

TPPP (untagged)-Human tubulin polymerization promoting protein (TPPP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Tubulin Polymerization Promoting Protein
• Synonyms: p24; p25; p25alpha; TPPP/p25; TPPP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115750 sequence for NM_007030 edited (data generated by NextGen Sequencing)
  ATGGCTGACAAGGCCAAGCCTGCCAAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGG
  GACCCCTCGAAGGACCGGGCAGCCAAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAG
  GGGGCAGCCGCATCCCCTGAGCTCAGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTG
  CACGGGGACGCCAGGGCCACCGGGAGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGC
  AAGGACTGCCAGGTGATCGACGGCAGGAACGTGACCGTCACTGACGTGGACATCGTCTTC
  AGCAAGATCAAAGGGAAGTCTTGCCGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTG
  GAGGAGCTCGCCAAGAAGCGATTCAAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTG
  CACAGGCTCATCGAGGGCAAGGCGCCCATCATCTCAGGGGTGACGAAAGCCATCTCGTCG
  CCCACAGTGTCGAGGCTCACGGACACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTC
  GACCCCTCTGGCAAGGGCAAGGGCAAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGC
  TATGTGTCCGGCTACAAGCACGCAGGCACCTACGACCAGAAGGTGCAAGGGGGCAAGTAG
  
  Clone variation with respect to NM_007030.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007030 unedited
  CACGAGGGCCTCCGCTGCAGTCGCTGCGTCTCCAACAACATGGCTGACAAGGCCAAGCCT
  GCCAAAGCTGCCAACAGGACGCCCCCCAAGTCCCCGGGGGACCCCTCGAAGGACCGGGCA
  GCCAAGAGGCTGTCGCTGGAATCGGAGGGTGCTGGTGAGGGGGCAGCCGCATCCCCTGAG
  CTCAGTGCCCTGGAGGAGGCCTTCCGGCGCTTTGCCGTGCACGGGGACGCCAGGGCCACC
  GGGAGGGAGATGCACGGCAAGAACTGGTCGAAGCTGTGCAAGGACTGCCAGGTGATCGAC
  GGCAGGAACGTGACCGTCACTGACGTGGACATCGTCTTCAGCAAGATCAAAGGGAAGTCT
  TGCCGGACCATCACCTTTGAGCAGTTCCAGGAGGCGCTGGAGGAGCTCGCCAAGAAGCGA
  TTCAAAGACAAGAGCAGCGAGGAGGCCGTTCGCGAGGTGCACAGGCTCATCGAGGGCAAG
  GCGCCCATCATCTCANGGGTGACGAAAGCCATCTCGTCGCCCACAGTGTCGAGGCTCACG
  GACACCACCAAGTTCACGGGCTCCCACAAGGAGCGCTTCGACCCCTCTGGCAAGGGCAAG
  GGCAAGGCTGGCCGCGTGGATCTGGTGGACGAGTCAGGCTATGTGTCCGGCTACAAGCAC
  GCAGGCACCTACGACCAGAANGGTGCAGGGGGCAAGTAGCCCCCGCTCCATGCCTCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007030
• Insert Size: 6200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007030.1, NP_008961.1
• RefSeq Size: 5019 bp
• RefSeq ORF: 660 bp
• Locus ID: 11076
• UniProt ID: O94811
• Cytogenetics: 5p15.33
• Gene Summary: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.[UniProtKB/Swiss-Prot Function]